Evaluation of Polycaprolactone-Associated Human Nasal Chondrocytes as a Therapeutic Agent for Cartilage Repair
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In this study, we manufactured a complex of human nasal septal cartilage (hNC) with polycaprolactone (PCL) for transplantation into cartilaginous skeletal defects and evaluated their characteristics.
Nasal septum tissue was obtained from five patients aged ≥ 20 years who were undergoing septoplasty. hNCs were isolated and subcultured for three passages in vitro. To formulate the cell–PCL complex, we used type I collagen as an adhesive between chondrocyte and PCL. Immunofluorescence staining, cell viability and growth in the hNC–PCL complex, and mycoplasma contamination were assessed.
hNCs in PCL showed viability ≥ 70% and remained at these levels for 9 h of incubation at 4 °C. Immunostaining of the hNC–PCL complex also showed high expression levels of chondrocyte-specific protein, COL2A1, SOX9, and aggrecan during 24 h of clinically applicable conditions.
The hNC–PCL complex may be a valuable therapeutic agent for implantation into injured cartilage tissue, and can be used clinically to repair cartilaginous skeletal defects. From a clinical perspective, it is important to set the short duration of the implantation process to achieve effective functional implantation.
KeywordsChondrocyte Collagen Human nasal septum Tissue engineering Cartilage
This research was supported by a grant (18172MFDS185) from Ministry of Food and Drug Safety of Korea 2019. This work was supported by the Korea Health Industry Development Institute funded by the Ministry of Health and Welfare (HI14C3228, HI14C0113), by the Basic Science Research Program through the National Research Foundation of Korea (NRF) grant funded by the Ministry of Education (2017R1D1A1B03027903, 2018R1D1A1B07045421), and by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science & ICT (2018M3A9E8020856). This work was also supported by the Institute of Clinical Medicine Research of Bucheon St. Mary’s Hospital, Research Fund, 2018. The sponsors had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Compliance with ethical standards
Conflict of interest
The authors indicate no potential conflict of interest.
All studies utilizing nasal septal chondrocytes were conducted following written approval (HC13TISI0038) from the Institutional Review Board of the Catholic Medical Center Clinical Research Coordinating Center, and after obtaining written informed consent from the donors.