Wicking Property of Graft Material Enhanced Bone Regeneration in the Ovariectomized Rat Model
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Recruitment and homing cells into graft materials from host tissue is crucial for bone regeneration.
Highly porous, multi-level structural, hydroxyapatite bone void filler (HA-BVF) have been investigated to restore critical size bone defects. The aim was to investigate a feasibility of bone regeneration of synthetic HA-BVF compared to commercial xenograft (Bio-Oss). HA-BVF of 0.7 mm in average diameter was prepared via template coating method. Groups of animals (n = 6) were divided into two with normal (Sham) or induced osteoporotic conditions (Ovx). Subsequently, subdivided into three treated with HA-BVF as an experiment or Bio-Oss as a positive control or no treatment as a negative control (defect). The new bone formation was analyzed by micro-CT and histology.
At 4 weeks post-surgery, new bone formation was initiated from all groups. At 8 weeks post-surgery, new bone formation in the HA-BVF groups was greater than Bio-Oss groups. Extraordinarily greater bone regeneration within the Ovx-HA group than Sham–Bio-Oss or Ovx–Bio-Oss group (p < 0.05).
This study suggests that the immediate wicking property of HA-BVF from host tissue activates a natural healing cascade without the addition of exogeneous factors or progenitor cells. HA-BVF may be an effective alternative for repairing bone defects under both normal and osteoporotic bone conditions.
KeywordsBone regeneration Hydroxyapatite Osteoporosis Bone void filler
This study was partially supported by Chonnam National University, 2016 and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by Ministry of Education (2017R1D1A1B03034829).
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
Animal experimental procedures were approved by the Institutional Animal Care and Use Committee of Chonnam National University (CNU IACUC-YB-R-2014-37) and the animals were cared for in accordance with the Guidelines for Animal Experiments of Chonnam National University.
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