Grafting collagen on poly (lactic acid) by a simple route to produce electrospun scaffolds, and their cell adhesion evaluation
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Increasing bioactivity and mechanical properties of polymers to produce more suitable scaffold for tissue engineering is a recurrent goal in the development of new biomedical materials. In this study, collagen-functionalized poly (lactic acid), PLA, was obtained by means of a simple grafting route, and electrospun scaffolds were produced to grow cells in vitro; their bioactivity was compared with scaffolds made of physical blends of PLA and collagen. Grafting was verified via nuclear magnetic resonance, attenuated total reflection-Fourier transform infrared and X-ray photoelectron spectroscopy. The cell adhesion performance of the scaffolds was studied using macrophages. Elastic modulus (74.7 megapascals) and tensile strength (3.0 megapascals) of the scaffold made from PLA grafted with collagen were substantially higher than the scaffolds made from physical blends of collagen and PLA: 32 and 2.16 megapascals, respectively, implying a more resistant material because of the chemical bond of the polypeptide to PLA. Besides, the fibers had more uniform diameter without defects. Scaffolds made from PLA grafted with collagen presented four-fold increase in cell adhesion than those of PLA blended with collagen. Furthermore, cell spreading within the scaffolds occurred only when collagen-functionalized poly (lactic acid) was used. These results open a new option for the easy tailoring of nanofiber-based scaffolds in three dimensions for tissue engineering.
Key WordsPoly (lactic acid) Collagen Grafting Electrospun scaffold Cell adhesion
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