Tissue Engineering and Regenerative Medicine

, Volume 13, Issue 4, pp 375–387 | Cite as

Grafting collagen on poly (lactic acid) by a simple route to produce electrospun scaffolds, and their cell adhesion evaluation

  • Alida Ospina-Orejarena
  • Ricardo Vera-Graziano
  • Maria Monica Castillo-Ortega
  • Juan Paulo Hinestroza
  • Mabel Rodriguez-Gonzalez
  • Laura Palomares-Aguilera
  • Marissa Morales-Moctezuma
  • Alfredo Maciel-Cerda
Original Article Tissue Engineering


Increasing bioactivity and mechanical properties of polymers to produce more suitable scaffold for tissue engineering is a recurrent goal in the development of new biomedical materials. In this study, collagen-functionalized poly (lactic acid), PLA, was obtained by means of a simple grafting route, and electrospun scaffolds were produced to grow cells in vitro; their bioactivity was compared with scaffolds made of physical blends of PLA and collagen. Grafting was verified via nuclear magnetic resonance, attenuated total reflection-Fourier transform infrared and X-ray photoelectron spectroscopy. The cell adhesion performance of the scaffolds was studied using macrophages. Elastic modulus (74.7 megapascals) and tensile strength (3.0 megapascals) of the scaffold made from PLA grafted with collagen were substantially higher than the scaffolds made from physical blends of collagen and PLA: 32 and 2.16 megapascals, respectively, implying a more resistant material because of the chemical bond of the polypeptide to PLA. Besides, the fibers had more uniform diameter without defects. Scaffolds made from PLA grafted with collagen presented four-fold increase in cell adhesion than those of PLA blended with collagen. Furthermore, cell spreading within the scaffolds occurred only when collagen-functionalized poly (lactic acid) was used. These results open a new option for the easy tailoring of nanofiber-based scaffolds in three dimensions for tissue engineering.

Key Words

Poly (lactic acid) Collagen Grafting Electrospun scaffold Cell adhesion 


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Copyright information

© The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Alida Ospina-Orejarena
    • 1
  • Ricardo Vera-Graziano
    • 1
  • Maria Monica Castillo-Ortega
    • 2
  • Juan Paulo Hinestroza
    • 3
  • Mabel Rodriguez-Gonzalez
    • 4
  • Laura Palomares-Aguilera
    • 4
  • Marissa Morales-Moctezuma
    • 1
  • Alfredo Maciel-Cerda
    • 1
    • 5
  1. 1.Instituto de Investigaciones en MaterialesUniversidad Nacional Autónoma de MéxicoMéxico, Distrito Federal C.P.México
  2. 2.Laboratorio de Química Sintética de Polímeros, Departamento de Investigación en Polímeros y MaterialesUniversidad de SonoraHermosillo, SonoraMéxico
  3. 3.Department of Fiber Science and Apparel DesignCornell UniversityIthacaUSA
  4. 4.Instituto de BiotecnologíaUniversidad Nacional Autónoma de MéxicoCuernavaca, MorelosMéxico
  5. 5.Instituto de Investigaciones en MaterialesUniversidad Nacional Autónoma de MéxicoMéxico, Distrito Federal C.P.México

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