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Applied Biological Chemistry

, Volume 60, Issue 2, pp 197–204 | Cite as

Anti-diabetic functional food with wasted litchi seed and standard of quality control

  • Se-Ah Choi
  • Ji Eun Lee
  • Min Ji Kyung
  • Ju Hee Youn
  • Jeong Bin Oh
  • Wan Kyunn Whang
Article

Abstract

The prevalence of diabetes has become a huge health burden in parts of quality of life and economic cost for overcoming this chronic disease. We followed a bioactivity-guided isolation using α-glucosidase inhibitory assay, four major compounds were isolated, and their structures were elucidated using nuclear magnetic resonance, mass spectrometry, and ultra-performance liquid chromatography quadrupole time of flight mass spectrometry in litchi seed. As a result, the IC50 of α-glucosidase inhibitory assay of the crude extract, sugar-removed layer, pavetannin B2, procyanidin A2, and acarbose was 0.691 μg/mL, 3.686 μg/mL, 0.04 μM, 0.08 μM, and 55.845 μg/mL, respectively. With those compounds, we examined the protein tyrosine phosphatase 1B inhibitory activity. And the IC50 of pavetannin B2, procyanidin A2, and ursolic acid was 450.295, 338.257, and 19.686 μM, respectively. Contents analysis method for bioactive compounds, which can be used in manufacturing for extract preparations, was established. The findings of this study, litchi seed can be a cost-effective medicinal food in terms of recyclable resources in the litchi food industry and as a natural alternative medicine against type-2 diabetes.

Keywords

α-Glucosidase inhibitory assay Anti-diabetic agent Bioactivity-guided isolation Litchi seed Protein tyrosine phosphatase 1B inhibitory assay Quality control standard 

Notes

Acknowledgments

This research was supported by the Chung-Ang University Graduate Research Scholarship in 2015.

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Copyright information

© The Korean Society for Applied Biological Chemistry 2017

Authors and Affiliations

  • Se-Ah Choi
    • 1
  • Ji Eun Lee
    • 1
  • Min Ji Kyung
    • 1
  • Ju Hee Youn
    • 1
  • Jeong Bin Oh
    • 1
  • Wan Kyunn Whang
    • 1
  1. 1.Pharmaceutical Botany Laboratory, College of PharmacyChung-Ang UniversityHeukseok-dong, Dongjak-gu, SeoulRepublic of Korea

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