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Applied Biological Chemistry

, Volume 59, Issue 4, pp 511–516 | Cite as

α-Pinene inhibits tumor invasion through downregulation of nuclear factor (NF)-κB-regulated matrix metalloproteinase-9 gene expression in MDA-MB-231 human breast cancer cells

  • Eunah Kang
  • Da Hyun Lee
  • You Jung Jung
  • Soon Young Shin
  • Dongsoo KohEmail author
  • Young Han LeeEmail author
Article

Abstract

2,6,6-Trimethylbicyclo[3.1.1]hept-2-ene (α-Pinene) is an organic compound of the terpene class found in the essential oil of many plants. In this study, the inhibitory effect of α-pinene on tumor invasion in highly metastatic MDA-MB-231 human breast cancer cells was evaluated. α-Pinene inhibited tumor necrosis factor (TNF)-α-induced invasiveness of MDA-MB-231 cells as revealed by three-dimensional spheroid invasion assay. Further analysis showed that α-pinene reduced TNFα-induced matrix metalloproteinase-9 gene promoter activation and mRNA expression in a dose-dependent manner. In addition, α-pinene treatment attenuated TNFα-induced nuclear factor κB (NF-κB) activation and NF-κB-dependent transcriptional activity. These results suggest that α-pinene has a significant effect on the inhibition of tumor invasion and may potentially be developed into an anti-metastatic drug.

Keywords

2,6,6-Trimethylbicyclo[3.1.1]hept-2-ene Breast cancer Matrix metalloproteinase-9 Nuclear factor kappa B Tumor invasion Tumor necrosis factor alpha 

Notes

Acknowledgments

This study was supported by the Konkuk University research support program.

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Copyright information

© The Korean Society for Applied Biological Chemistry 2016

Authors and Affiliations

  1. 1.Department of Biological Sciences, College of Biological Science and BiotechnologyKonkuk UniversitySeoulRepublic of Korea
  2. 2.Cancer and Metabolism InstituteKonkuk UniversitySeoulRepublic of Korea
  3. 3.Department of Applied ChemistryDongduk Women’s UniversitySeoulRepublic of Korea

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