Inhibition of Botrytis cinerea spore germination and mycelia growth by frequency-specific sound
The effect of sound waves on mycelial growth of Botrytis cinerea was investigated to explore whether frequency-specific sound could be used as a practical alternative to chemical fungicides to control plant diseases. The fungus was exposed to wave frequencies ranging from 1 to 5 kHz, and then observed using light and scanning electron microscopy to assess changes in several physiological and morphological aspects. Of the frequencies tested, 5 kHz sound wave significantly inhibited mycelial growth and spore germination. Furthermore, morphological changes, including low mycelial density, swollen mycelial tips, and irregular mycelial surfaces, were observed. Most internal hyphae were empty, and the ends of hyphae were significantly thinner or swollen. These observations suggest that 5 kHz sound waves create stressful growth conditions for the fungus, which leads to the inhibition of mycelia growth and spore germination. It is possible that sound wave treatment could represent an environmentally-friendly alternative to chemical fungicides. These results broaden our knowledge regarding the effective management of noxious nectrotrophic fungal pathogens by a nonchemical approach.
Keywordsbiocontrol botrytis cinerea frequency-specific sound inhibition mycelia growth spore germination
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- Braam J, Sistrunk ML, Sistrunk, DH Xu, Purugganan MM, Antosiewicz DM et al. (1977) Plant response to environmental stress: Regulation and function of the Arabidopsis TCH genes. Planta 203, S53–41.Google Scholar
- Braam J (1992) Regulated expression of the calmodulin-related TCH genes in cultures Arabidopsis cells: Induction by calcium and heat shock. PNAS 80, 3212–3216.Google Scholar
- Galston AW and Slayman CL (1979) The not-so-secret life of plants. Am Aci 67, 337–344.Google Scholar
- Hongbo S, Biao L, Bochu W, Kun T, and Yilong L (2008) A study on differentially expressed gene screening of Chrysanthemum plants under sound stress. Colloids and surfaces B: Biointerfaces 331, 329–333.Google Scholar
- Jarvis WR (1977) Botryotinia and Botrytis Species-Taxonomy, Physiology and Pathogenicity-a Guide to Literature. Canada Department of Agriculture, Ca.Google Scholar
- Jomdecha C and Prateepasen A (2011) Effects of pulse ultrasonic irradiation on the lag phase of Saccharomyces cerevisiae growth. Letters in Applied 52(1), 62–69.Google Scholar
- Mizoguchi T, Irie K, Hirayama T, Hayashida N, Yamaguchi-Shinozaki K, Matsumoto K et al. (1996) A gene encoding a mitogen-activated protein kinase is induced simultaneously with genes for a mitogen-activated protein kinase and an S6 ribosomal protein kinase by touch, cold, and water stress in Arabidopsis thaliana. PNAS 93, 765–769.CrossRefGoogle Scholar
- Prins TW, Tudzynski P, von Tiedemann A, Tudzynski B, Have A, Hansen ME et al. (2000) Infection strategies of Botrytis cinerea and related necrotrophic pathogens. In: Fungal Pathology, J. Kronstad (ed.), pp. 3–34, Kluwer Academic Publishers, The Netherlands.Google Scholar
- Sistrunk ML, Antosiewica DM, Purugganan MM, and Braam J (1994) The Arabidopsis TCH3 encodes a novel Ca2+ binding protein and shows environmentally induced and tissue-specific regulation. Plant Cell 6, 1553–1565.Google Scholar
- Takahashi H, Suge H, and Kato T (1992) Growth promotion by vibration at 50 Hz in rice and cucumber seedlings. Plant Cell Physiol 32, 729–732.Google Scholar