Cytotoxic property of ultraviolet-induced rice phytoalexins to human colon carcinoma HCT-116 cells

  • Jong-Hwa Park
  • Yuan-Yuan Fu
  • In Sik Chung
  • Tae-Ryong Hahn
  • Man-Ho Cho
Short Communication
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Accepted:

Abstract

Exposure to ultraviolet (UV) radiation increased the cytotoxic effect of rice leaf extract on human colon carcinoma HCT-116 cells, suggesting that the production of cytotoxic compounds in rice leaves are induced by UV treatment. To identify cytotoxic agents in UV-treated rice leaves, the phenolic compound peaks prominently increased by UV treatment were isolated using reversed phase high performance liquid chromatography (HPLC). The isolated compounds were identified as N-trans-cinnamoyltyramine, N-benzoyltryptamine, and sakuranetin using NMR and mass spectrometric techniques. N-Benzoyltryptamine and sakuranetin were previously reported as rice phytoalexins, and N-trans-cinnamoyltyramine was first isolated from rice. N-Benzoyltryptamine and sakuranetin were found to inhibit the growth of HCT-116 cells with IC50 values of 89.2±4.8 and 68.8±5.2 μg/mL, respectively, indicating that these phytoalexins are cytotoxic agents in the UV-treated rice leaves. These results suggest that rice phytoalexins are potent anti-cancer agents and UV-treated rice leaves are valuable source for therapeutic agents.

Keywords

anti-cancer property N-benzoyltryptamine N-trans-cinnamoyltyramine rice phytoalexin sakuranetin ultraviolet radiation 
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Copyright information

© The Korean Society for Applied Biological Chemistry 2013

Authors and Affiliations

  • Jong-Hwa Park
    • 1
  • Yuan-Yuan Fu
    • 1
  • In Sik Chung
    • 1
  • Tae-Ryong Hahn
    • 1
  • Man-Ho Cho
    • 1
  1. 1.Graduate School of Biotechnology and Plant Metabolism Research CenterKyung Hee UniversityYonginRepublic of Korea

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