Isolation of auxin- and 1-aminocyclopropane-1-carboxylic acid deaminase-producing bacterium and its effect on pepper growth under saline stress

  • Jong-Hui Lim
  • Chang-Hwan An
  • Yo-Hwan Kim
  • Byung-Kwon Jung
  • Sang-Dal Kim
Original Article Environmental Sciences
First Online:
Received:
Accepted:

Abstract

Plant tissues produce ethylene under the environmental stresses such as drought, salinity, and heavy metals. Ethylene concentration can be reduced by 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase produced by plant growth-promoting rhizobacterium (PGPR), which cleaves the ethylene precursor ACC. The present study focused on alleviation of environmental stress by selected PGPR, which could suppress fungal plant disease. These PGPRs were capable of utilizing ACC as sole source of nitrogen and also produced auxin. Seed germination of red pepper was reduced with increasing salt concentration, and approximately 98.2% of seeds germinated in the absence of salt, whereas only 36.2% seeds germinated in the presence of 175 mM NaCl. Seed germination was also decreased by 62.1 and 19.9% in the presence of 120mM NaCl and 120mM NaCl +ACC deaminase-producing PGPR Pseudomonas fluorescens 2112, respectively, compared to uninoculated control. The effect of salinity stress with different salt concentration on pepper plants and their alleviation with PGPR was evaluated. Non-inoculated pepper plants died after 5 week when grown in the presence of high salt (120 mM NaCl), whereas 80% of pepper plants inoculated with P. fluorescens 2112 survived under the high salt stress. Salt stress also decreased the fresh and dry weights of pepper grown, compared to the negative control, whereas pepper plants inoculated with P. fluorescens 2112 retained the biomass similar to control plants. These results indicate that ACC deaminase and auxin producing P. fluorescens 2112 is a multi-functional PGPR that can promote the growth and development of pepper plants by alleviating the high-salt stress.

Keywords

1-aminocyclopropane-1-carboxylic acid deaminase plant growth promoting rhizobacteria Pseudomonas fluorescens saline stress 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bleecker AB and Kende H (2000) Ethylene: a gaseous signal molecule in plants. Annu Rev Cell Dev Biol 16, 1–18.CrossRefGoogle Scholar
  2. Cheng Z, Park E, and Glick BR (2007) 1-Aminocyclopropane-1-carboxylate deaminase from Pseudomonas putida UW4 facilitates the growth of canola in the presence of salt. Can J Microbiol 53, 912–918.CrossRefGoogle Scholar
  3. Dimkpa C, Weinand T, and Asch F (2009) Plant-rhizobacteria interactions alleviate abiotic stress conditions. Plant Cell Environ 32, 1682–1694.CrossRefGoogle Scholar
  4. Egamberdieva D, Kamilova F, Validov S, Gafurova L, Kucharova1 Z, and Lugtenberg B (2008) High incidence of plant growth-stimulating bacteria associated with the rhizosphere of wheat grown on salinated soil in Uzbekistan. Environ Microbiol 10, 1–9.Google Scholar
  5. Glick BR (1995) The enhancement of plant growth by free-living bacteria. Can J Microbiol 41, 109–117.CrossRefGoogle Scholar
  6. Glick BR (2004) Bacterial ACC deaminase and the alleviation of plant stress. Adv Appl Microbiol 56, 291–312.CrossRefGoogle Scholar
  7. Glick BR (2005) Modulation of plant ethylene levels by the bacterial enzyme ACC Deaminase. FEMS Microbiol Lett 251, 1–7.CrossRefGoogle Scholar
  8. Jacobson CB, Pasternak JJ, and Glick BR (1994) Partial purification and characterization of ACC deaminase from the plant growth-promoting rhizobacterium Pseudomonas putida GR12-2. Can J Microbiol 40, 1019–1025.CrossRefGoogle Scholar
  9. Jalili F, Khavazi K, Pazira E, Nejati A, Rahmani HA, Sadaghiani HR et al. (2009) Isolation and characterization of ACC deaminase-producing fluorescent pseudomonads, to alleviate salinity stress on canola (Brassica napus L.) growth. J Plant Physiol 166, 667–674.CrossRefGoogle Scholar
  10. Jang J, Kloepper JW, and Ryu CM (2009) Rhizosphere bacteria help plants tolerate abiotic stress. Trends Plant Sci 14, 1360–1385.Google Scholar
  11. Jung HK and Kim SD (2003) Purification and characteriztion of an antifungal antibiotic from Bacillus megaterium KL 39, a biocontrol agent of redpepper Phytophtora blight disease. Korean J Microbiol Biotechnol 31, 235–241.Google Scholar
  12. Jung HK, Kim JR, Kim BK, Yu TS, and Kim SD (2003) Selection and antagonistic mechanism of Bacillus thuringiensis BK4 against fusarium wilt disease of tomato. Korean J Microbiol Biotechnol 33, 194–199.Google Scholar
  13. Kim JR (2004) Isolation and characteristics research of an auxin and hydrolytic enzyme by plant growth promotion rhizobacteria (PGPR). MS Thesis, Yeungnam University, Gyeongsan, Korea.Google Scholar
  14. Kloepper JW, Gutierrez-Estrada A, and Mclnory JA (2007) Photoperiod regulates elicitation of growth promotion but not induced resistance by plant growth-promoting rhizobacteria. Can J Microbiol 53, 159–167.CrossRefGoogle Scholar
  15. Kloepper JW, Ryu CM, and Zhang S (2004) Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopathology 94, 1259–1266.CrossRefGoogle Scholar
  16. Lee ET and Kim SD (1999) Isolation and antifungal activity of the chitinase producing bacterium Serratia sp. 3095 as antagonistic bacterium against Fusarium sp. J Korean Soc Agric Chem Biotechnol 42, 181–187.Google Scholar
  17. Lee ET and Kim SD (2001) An antifungal substance, 2,4-diacetylphloroglucinol, produced from antagonistic bacterium Pseudomonas fluorescens 2112 against Phytophthora capsici. Korean J Microbiol Biotechnol 29, 37–42.Google Scholar
  18. Li J, Ovakim DH, Charles TC, and Glick BR (2000) An ACC Deaminase minus mutant of Enterobacter cloacae UW4 no longer promotes root elongation. Curr Microbiol 41, 101–105.CrossRefGoogle Scholar
  19. Lim JH and Kim SD (2009) Synergistic plant growth promotion by indigenous auxin-producing PGPR Bacillus subtilis AH18 and Bacillus licheniformis K11. J Korean Soc Appl Biol Chem 52, 231–538.Google Scholar
  20. Lowry OH, Rosebrough NJ, Farr AL, and Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193, 265–275.Google Scholar
  21. Mayak S, Tirosh T, and Glick BR (1999) Effect of wild-type and mutant plant growth promoting rhizobacteria on the rooting of mung bean cuttings. J Plant Growth Regul 18, 49–53.CrossRefGoogle Scholar
  22. Mayak S, Tirosh T, and Glick BR (2001) Stimulation of the growth of tomato, pepper and mung bean plants by the plant growth-promoting bacterium Enterobacter cloacae CAL3. Biol Agric Hort 19, 261–274.CrossRefGoogle Scholar
  23. Mayak S, Tirosh T, and Glick BR (2004) Plant growth-promoting bacteria confer resistance in tomato plants to salt stress. Plant Physiol Biochem 42, 565–572.CrossRefGoogle Scholar
  24. Nadeem SM, Zahir ZA, Naveed M, and Arshad M (2007) Preliminary investigations on inducing salt tolerance in maize through inoculation with rhizobacteria containing ACC deaminase activity. Can J Microbiol 53, 1141–1149.CrossRefGoogle Scholar
  25. O’Donnell PJ, Calvert C, Atzorn R, Wasternack C, Leyser HMO, and Bowles DJ (1996) Ethylene as a signal mediating the wound response of tomato plants. Science 274, 1914–1917.CrossRefGoogle Scholar
  26. Patten CL and Glick BR (2002) Regulation of indoleacetic acid production in Pseudomonas putida GR12-2 by tryptophan and the stationary-phase sigma factor RpoS. Can J Microbiol 48, 635–642.CrossRefGoogle Scholar
  27. Penrose DM and Glick BR (2003) Methods for isolating and characterizing ACC deaminase-containing plant growth promoting rhizobacteria. Physiol Plant 118, 10–15.CrossRefGoogle Scholar
  28. Saravanakumar D and Samiyappan R (2007) ACC deaminase from Pseudomonas fluorescens mediated saline resistance in groundnut (Arachis hypogaea) plants. J Appl Microbiol 102, 1283–1292.CrossRefGoogle Scholar
  29. Siddikee MA, Chauhan PS, Anandham R, Han GH, and Sa T (2010) Isolation, characterization, and use for plant growth promotion under salt stress, of ACC deaminase-producing halotolerant bacteria derived from coastal soil. J Microbiol Biotechnol 20, 1577–1584.CrossRefGoogle Scholar
  30. Siddikee MA, Glick BR, Chauhan PS, Yim WJ, and Sa T (2011) Enhancement of growth and salt tolerance of red pepper seedlings (Capsicum annuum L.) by regulating stress ethylene synthesis with halotolerant bacteria containing 1-aminocyclopropane-1-carboxylic acid deaminase activity. Plant Physiol Biochem 49, 427–434.CrossRefGoogle Scholar
  31. Yun GH, Lee ET, and Kim SD (2001) Identification and antifungal antagonism of Chryseomonas luteola 5042 against Phytophthora capsici. Korean J Appl Microbiol Biotechnol 29, 186–193.Google Scholar
  32. Zhu JK (2001) Plant salt tolerance. Trends Plant Sci 6, 66–71.CrossRefGoogle Scholar

Copyright information

© The Korean Society for Applied Biological Chemistry 2012

Authors and Affiliations

  • Jong-Hui Lim
    • 1
    • 2
  • Chang-Hwan An
    • 1
  • Yo-Hwan Kim
    • 1
  • Byung-Kwon Jung
    • 1
  • Sang-Dal Kim
    • 1
  1. 1.School of BiotechnologyYeungnam UniversityGyeongbukRepublic of Korea
  2. 2.School of Applied BioscienceKyungpook National UniversityDaeguRepublic of Korea

Personalised recommendations