Inhibitory effect of galanolactone isolated from Zingiber officinale roscoe extract on adipogenesis in 3T3-L1 cells

Original Article Bioactive Materials


Zingiber officinale Roscoe commonly known as ginger, has been used in traditional medicine. Inhibtion effect of galanolactone isolated from Z. officinale Roscoe on adipogenesis in 3T3-L1 cells was evaluated. Effect of galanolactone on 3T3-L1 adipocyte differentiation was measured by Oil Red O staining, and cytotoxicity effect of galanolactone was analyzed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay. The expression of various genes involved in adipogenic action of galanolactone was determined by real-time PCR and Western blot. Peroxisome proliferator-activated receptor γ (PPARγ) luciferase transactivation assay was used to evaluate the PPARγ transcriptional activity of galanolactone in HEK 293T cells. Galanolactone inhibited lipid accumulation and expression of adipocyte fatty acid-binding protein (aP2) and resistin in a dose-dependent manner in 3T3-L1 cells. Treatment with 50 and 100 μM of galanolactone significantly decreased the troglitazone-induced PPARγ transcripitional activity in HEK 293T cells, and suppressed expressions of PPARγ and CCAAT-enhancer-binding protein α (C/EBPα) at mRNA and protein levels in 3T3-L1 cells. These findings suggest that galanolactone isolated from Z. officinale Roscoe exerts anti-obesity effect through downregulation of adipogenic transcription factors and adipogenic marker genes.


adipogenesis adipogenic marker genes galanolactone peroxisome proliferator-activated receptor γ 3T3-L1 cells Zingiber officinale 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Afzal M, Al HD, Menon M, Pesek M, and Dharmi M (2001) Ginger an ethnomedical, chemical and pharmacological review. Drug Metab Drug Interact 18, 159–190.CrossRefGoogle Scholar
  2. Bhandari U, Sharma JN, and Zafar R (1998) The protective action of ethanolic ginger extract in cholesterol-fed rabbits. J Ethnopharmacol 61, 167–171.CrossRefGoogle Scholar
  3. Burn RP, Kim JB, Hu E, Altiok S, and Spiegelman BM (1996) Adipocyte differentiation a transcriptional regulatory cascade. Curr Opin Cell Biol 8, 826–832.CrossRefGoogle Scholar
  4. Chen BH, Wu PY, Chen KM, Fu TF, Wang HM, and Chen CY (2009) Antiallergic Potential on RBL-2H3 Cells of Some Phenolic Constituents of Zingiber officinale. J Nat Prod 72, 950–953.CrossRefGoogle Scholar
  5. Chrubasik S, Pittler MH, and Roufogalis BD (2005) Zingiberis rhizoma a comprehensive review on the ginger effect and efficacy profiles. Phytomedicine 12, 684–701.CrossRefGoogle Scholar
  6. Fuhrman B, Rosenblat M, Hayek T, Coleman R, and Aviram M (2000) Ginger extract consumption reduces plasma cholesterol, inhibits LDL oxidation and attenuates development of atherosclerosis in atherosclerotic, apolipoprotein E-deficient mice. J Nutr 130, 1124–1131.Google Scholar
  7. Gregoire FM, Smas CM, and Sul HS (1998) Understanding adipocyte differentiation. Physiol Rev 78, 789–809.Google Scholar
  8. Han L, Gong X, Kawano S, Saito M, Kimura Y, and Okuda H (2005) Antiobesity actions of Zingiber officinale Roscoe. Yakugaku Zasshi 125, 213–217.CrossRefGoogle Scholar
  9. Hu E, Kim J, Sarraf P, and Spiegelman B (1996) Inhibition of adipogenesis through MAP Kinase-mediated phosphorylation of PPAR gamma. Science 274, 2100–2103.CrossRefGoogle Scholar
  10. Hwang JT, Lee MS, Kim HJ, Sung MJ, Kim HY, Kim MS, and Kwon DY (2009) Antiobesity effect of ginsenoside Rg3 involves the AMPK and PPAR-gamma signal pathways. Phytother Res 23, 262–266.CrossRefGoogle Scholar
  11. Jeon T, Hwang SG, Hirai S, Matsui T, Yano H, Kawada T, Lim BU, and Ki D (2004) Red yeast rice extracts suppress adipogenesis by down-regulating adipogenic transcription factors and gene expression in 3T3-L1 cells. Life Sci 75, 3195–3203.CrossRefGoogle Scholar
  12. Kikuzaki H, Tsai SM, and Nakatani N (1992) Gingerdiol related compounds from the rhizomes of Zingiber officinale. Phytochemistry 31, 1783–1786.CrossRefGoogle Scholar
  13. Kubota N, Terauchi Y, Miki H, Tamemoto H, Yamauchi T, Komeda K, Satoh S, Nakano R, Ishii C, Sugiyama T, Eto K, Tsubamoto Y, Okuno A, Murakami K, Sekihara H, Hasegawa G, Naito M, Toyoshima Y, Tanaka S, Shiota K, Kitamura T, Fujita T, Ezaki O, Aizawa S, Nagai R, Tobe K, Kimura S, and Kadowaki T (1999) PPAR gamma mediates high-fat dietinduced adipocyte hypertrophy and insulin resistance. Mol cell 4, 597–609.CrossRefGoogle Scholar
  14. Lehmann JM, Moore LB, Smith-Oliver TA, Wilkison WO, Willson TM, and Kliewer SA (1995) An antidiabetic thiazolidinedione is a high affinity ligand for peroxisome proliferator-activated receptor γ (PPARγ). J Biol Chem 270, 12953–12956.Google Scholar
  15. Lowell BB (1999) PPARγ: An essential regulator of adipogenesis and modulator of fat cell function. Cell 99, 239–242.CrossRefGoogle Scholar
  16. Mandrup S and Lane MD (1997) Regulating Adipogenesis. J Biol Chem 272, 5367–5370.CrossRefGoogle Scholar
  17. Morrison RF and Farmer SR (2000) Hormonal Signaling and Transcriptional Control of Adipocyte Differentiation. J Nutr 130, 3116–3121.Google Scholar
  18. Rajala MW, Obici S, Scherer PE, and Rossetti L (2003) Adipose-derived resistin and gut-derived resistin-like molecule-beta selectively impair insulin action on glucose production. J Clin Invest 111, 225–230.Google Scholar
  19. Rosen ED, Walkey CJ, Puigserver P, and Spiegelman BM (2000) Transcriptional regulation of adipogenesis. Genes & Dev 14, 1293–1307.Google Scholar
  20. Sang S, Hong J, Wu H, Liu J, Yang CS, Pan MH, Badmaev V, and Ho CT (2009) Increased growth inhibitory effects on human cancer cells and anti-inflammatory potency of shogaols from Zingiber officinale relative to gingerols. J Agric Food Chem 22, 10645–10650.CrossRefGoogle Scholar
  21. Steppan CM, Bailey ST, Bhat S, Brown EJ, Banerjee RR, Wright CM, Patel HR, Ahima RS, and Lazar MA (2001) The hormone resistin links obesity to diabetes. Nature 409, 307–312.CrossRefGoogle Scholar
  22. Tontonoz P, Hu E, Devine J, Beale EG, and Spiegelman BM (1995) PPARγ2 regulates adipose expression of the phosphoenolpyruvate carboxykinase gene. Mol Cell Biol 15, 351–357.Google Scholar
  23. Tontonoz P, Hu E, Graves RA, Budavari AI, and Spiegelman BM (1994) mPPAR72 tissue-specific regulator of an adipocyte enhancer. Genes & Dev 8, 1224–1234.CrossRefGoogle Scholar

Copyright information

© The Korean Society for Applied Biological Chemistry 2012

Authors and Affiliations

  1. 1.Natural Products Research InstituteGyeonggi Institute of Science & Technology PromotionGyeonggi-doRepublic of Korea
  2. 2.College of PharmacyDankook UniversityCheonan-siRepublic of Korea

Personalised recommendations