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High-efficient biodegradation of refractory dye by a new bacterial flora DDMY1 under different conditions

  • X. H. XieEmail author
  • X. L. Zheng
  • C. Z. Yu
  • Q. Y. Zhang
  • Y. Q. Wang
  • J. H. Cong
  • N. Liu
  • Z. J. He
  • B. Yang
  • J. S. Liu
Original Paper
  • 1 Downloads

Abstract

Industrial wastewater with refractory dyes is still considered as one kind of serious pollution. Biological treatments have shown great potential for the biodegradation of dyes. A new natural bacterial flora DDMY1 with efficient decolorization performance of azo dye reactive black 5 (RB5) was obtained from activated sludge by concentration gradient domestication. Morphological analysis, growth test, decolorization assay of different parameters, kinetics study and high-throughput sequencing were performed in this study. Morphological analysis showed the features of flora DDMY1 and growth curves emerged the temperature range suitable for the growth of flora DDMY1. Results of decolorization assays indicated that xylose and yeast extract could enhance the decolorization effect and flora DDMY1 could decolorize RB5 not only in wide ranges of pH values (4.0–9.0) and initial dye concentration (100–1000 mg L−1), but also in the optimum temperature (40 °C) and NaCl concentration (1.5%, w/v). Kinetics study of RB5 decolorization at 30–40 °C conformed to the first-order kinetic reaction equation. High-throughput sequencing analysis revealed the community structure of flora DDMY1 and the dominant genus unclassified_o_Pseudomonadales, which had synergistic effect on decolorization with unclassified_f_Alcaligenaceae, Pseudomonas and other genera. Findings of this study revealed a sort of natural bacterial flora that could accommodate different wastewater conditions and remove high-concentration refractory dyes efficiently. These results also indicated the possibility of treating dyeing wastewater on a large scale with different conditions.

Keywords

Biodegradation Decolorization High-throughput sequencing Natural bacterial flora DDMY1 Reactive black 5 

Notes

Acknowledgements

The authors acknowledge the financial support by the Fundamental Research Funds for the Central Universities (2232018G-11, 2232019D3-22), the National Key Research and Development Program of China (Grant No. 2016YFC0400501), the Graduate Student Innovation Fund of Donghua University (CUSF-DH-D-2019078), Anhui Provincial Natural Science Foundation (1808085QE176), the scientific research program of Anhui Provincial Education Department (KJ2018A0444) and the Suzhou University Startup Foundation for Doctor (2016jb04), the “Chenguang Program” supported by Shanghai Education Development Foundation and Shanghai Municipal Education Commission (No. 16CG40). This work was partially supported by Shanghai Leading Academic Discipline Project (B604).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13762_2019_2582_MOESM1_ESM.docx (83 kb)
Supplementary material 1 (DOCX 83 kb)

References

  1. Amato KR, Yeoman CJ, Kent A, Righini N, Carbonero F, Estrada A, Gaskins HR, Stumpf RM, Yildirim S, Torralba M, Gillis M, Wilson BA, Nelson KE, White BA, Leigh SR (2013) Habitat degradation impacts black howler monkey (Alouatta pigra) gastrointestinal microbiomes. ISME J 7:1344–1353CrossRefGoogle Scholar
  2. Balapure KH, Jain K, Chattaraj S, Bhatt NS, Madamwar D (2014) Co-metabolic degradation of diazo dye-Reactive blue 160 by enriched mixed cultures BDN. J Hazard Mater 279:85–95CrossRefGoogle Scholar
  3. Bilinska L, Blus K, Gmurek M, Ledakowicz S (2019) Coupling of electrocoagulation and ozone treatment for textile wastewater reuse. Chem Eng J 358:992–1001CrossRefGoogle Scholar
  4. Cao D-J, Wang J-J, Zhang Q, Wen Y-Z, Dong B, Liu R-J, Yang X, Geng G (2019a) Biodegradation of triphenylmethane dye crystal violet by Cedecea davisae. Spectrochim Acta A 210:9–13CrossRefGoogle Scholar
  5. Cao J, Sanganyado E, Liu W, Zhang W, Liu Y (2019b) Decolorization and detoxification of Direct Blue 2B by indigenous bacterial consortium. J Environ Manag 242:229–237CrossRefGoogle Scholar
  6. Chacko JT, Subramaniam K (2011) Enzymatic degradation of azo dyes—a review. Int J Environ Sci 1:1250–1260Google Scholar
  7. Cheng S, Li N, Jiang L, Li Y, Xu B, Zhou W (2019) Biodegradation of metal complex Naphthol Green B and formation of iron-sulfur nanoparticles by marine bacterium Pseudoalteromonas sp. CF10-13. Bioresour Technol 273:49–55CrossRefGoogle Scholar
  8. Dafale N, Wate S, Meshram S, Nandy T (2008) Kinetic study approach of remazol black-B use for the development of two-stage anoxic-oxic reactor for decolorization/biodegradation of azo dyes by activated bacterial consortium. J Hazard Mater 159:319–328CrossRefGoogle Scholar
  9. Donadelli JA, Carlos L, Arques A, Garcia Einschlag FS (2018) Kinetic and mechanistic analysis of azo dyes decolorization by ZVI-assisted Fenton systems: pH-dependent shift in the contributions of reductive and oxidative transformation pathways. Appl Catal B Environ 231:51–61CrossRefGoogle Scholar
  10. dos Santos AB, Cervantes FJ, van Lier JB (2007) Review paper on current technologies for decolourisation of textile wastewaters: perspectives for anaerobic biotechnology. Bioresour Technol 98:2369–2385CrossRefGoogle Scholar
  11. Elwakeel KZ (2009) Removal of Reactive Black 5 from aqueous solutions using magnetic chitosan resins. J Hazard Mater 167:383–392CrossRefGoogle Scholar
  12. Elwakeel KZ, Rekaby M (2011) Efficient removal of Reactive Black 5 from aqueous media using glycidyl methacrylate resin modified with tetraethelenepentamine. J Hazard Mater 188:10–18CrossRefGoogle Scholar
  13. Elwakeel KZ, Abd El-Ghaffar MA, El-Kousy SM, El-Shorbagy HG (2013) Enhanced remediation of Reactive Black 5 from aqueous media using new chitosan ion exchangers. J Disper Sci Technol 34:1008–1019CrossRefGoogle Scholar
  14. Elwakeel KZ, El-Kousy S, El-Shorbagy HG, El-Ghaffar MAA (2016) Comparison between the removal of Reactive Black 5 from aqueous solutions by 3-amino-1,2,4 triazole,5-thiol and melamine grafted chitosan prepared through four different routes. J Environ Chem Eng 4:733–745CrossRefGoogle Scholar
  15. Eskandari F, Shahnavaz B, Mashreghi M (2019) Optimization of complete RB-5 azo dye decolorization using novel cold-adapted and mesophilic bacterial consortia. J Environ Manag 241:91–98CrossRefGoogle Scholar
  16. Farha AK, Tr T, Purushothaman A, Salam JA, Hatha AM (2018) Phylogenetic diversity and biotechnological potentials of marine bacteria from continental slope of eastern Arabian Sea. J Genet Eng Biotechnol 16:253–258CrossRefGoogle Scholar
  17. Franciscon E, Zille A, Fantinatti-Garboggini F, Silva IS, Cavaco-Paulo A, Durrant LR (2009) Microaerophilic-aerobic sequential decolourization/biodegradation of textile azo dyes by a facultative Klebsiella sp. strain VN-31. Process Biochem 44:446–452CrossRefGoogle Scholar
  18. Gola D, Malik A, Namburath M, Ahammad SZ (2018) Removal of industrial dyes and heavy metals by Beauveria bassiana: FTIR, SEM, TEM and AFM investigations with Pb(II). Environ Sci Pollut Res 25:20486–20496CrossRefGoogle Scholar
  19. Hashem RA, Samir R, Essam TM, Ali AE, Amin MA (2018) Optimization and enhancement of textile reactive Remazol black B decolorization and detoxification by environmentally isolated pH tolerant Pseudomonas aeruginosa KY284155. AMB Express 8:83CrossRefGoogle Scholar
  20. Holkar CR, Arora H, Halder D, Pinjari DV (2018) Biodegradation of reactive blue 19 with simultaneous electricity generation by the newly isolated electrogenic Klebsiella sp. C NCIM 5546 bacterium in a microbial fuel cell. Int Biodeterior Biodegrad 133:194–201CrossRefGoogle Scholar
  21. Hussain S, Maqbool Z, Ali S, Yasmeen T, Imran M, Mahmood F, Abbas F (2013) Biodecolorization of reactive black-5 by a metal and salt tolerant bacterial strain Pseudomonas sp. RA20 isolated from Paharang drain effluents in Pakistan. Ecotoxicol Environ Saf 98:331–338CrossRefGoogle Scholar
  22. Imran M, Arshad M, Negm F, Khalid A, Shaharoona B, Hussain S, Nadeem SM, Crowley DE (2016) Yeast extract promotes decolorization of azo dyes by stimulating azoreductase activity in Shewanella sp. strain IFN4. Ecotoxicol Environ Saf 124:42–49CrossRefGoogle Scholar
  23. Kalyani DC, Telke AA, Dhanve RS, Jadhav JP (2009) Ecofriendly biodegradation and detoxification of Reactive Red 2 textile dye by newly isolated Pseudomonas sp. SUK1. J Hazard Mater 163:735–742CrossRefGoogle Scholar
  24. Karim ME, Dhar K, Hossain MT (2017) Co-metabolic decolorization of a textile reactive dye by Aspergillus fumigatus. Int J Environ Sci Technol 14:177–186CrossRefGoogle Scholar
  25. Khan S, Malik A (2018) Toxicity evaluation of textile effluents and role of native soil bacterium in biodegradation of a textile dye. Environ Sci Pollut Res 25:4446–4458CrossRefGoogle Scholar
  26. Khan Z, Jain K, Soni A, Madamwar D (2014) Microaerophilic degradation of sulphonated azo dye—Reactive Red 195 by bacterial consortium AR1 through co-metabolism. Int Biodeterior Biodegrad 94:167–175CrossRefGoogle Scholar
  27. Khorram AG, Fallah N (2019) Comparison of sludge settling velocity and filtration time after electrocoagulation process in treating industrial textile dyeing wastewater: RSM optimization. Int J Environ Sci Technol 16:3437–3446CrossRefGoogle Scholar
  28. Li Q, Liao Z, Fang X, Wang D, Xie J, Sun X, Wang L, Li J (2019) Tannic acid-polyethyleneimine crosslinked loose nanofiltration membrane for dye/salt mixture separation. J Membr Sci 584:324–332CrossRefGoogle Scholar
  29. Lima Beluci NdC, Pisano Mateus GA, Miyashiro CS, Homem NC, Gomes RG, Fagundes-Klen MR, Bergamasco R, Salcedo Vieira AM (2019) Hybrid treatment of coagulation/flocculation process followed by ultrafiltration in TiO2-modified membranes to improve the removal of reactive black 5 dye. Sci Total Environ 664:222–229CrossRefGoogle Scholar
  30. Liu N, Xie X, Yang B, Zhang Q, Yu C, Zheng X, Xu L, Li R, Liu J (2017a) Performance and microbial community structures of hydrolysis acidification process treating azo and anthraquinone dyes in different stages. Environ Sci Pollut Res 24:252–263CrossRefGoogle Scholar
  31. Liu Y-N, Zhang F, Li J, Li D-B, Liu D-F, Li WW, Yu H-Q (2017b) Exclusive extracellular bioreduction of methyl orange by azo reductase-Free Geobacter sulfurreducens. Environ Sci Technol 51:8616–8623CrossRefGoogle Scholar
  32. Lops C, Ancona A, Di Cesare K, Dumontel B, Garino N, Canavese G, Hérnandez S, Cauda V (2019) Sonophotocatalytic degradation mechanisms of Rhodamine B dye via radicals generation by micro- and nano-particles of ZnO. Appl Catal B Environ 243:629–640CrossRefGoogle Scholar
  33. Mahdizadeh F, Aber S, Dehghan G (2018) Removal of phenol from aqueous solution using the green Macroalga Chara sp. Clean-Soil Air Water 46:1800181CrossRefGoogle Scholar
  34. Mahmood F, Shahid M, Hussain S, Shahzad T, Tahir M, Ijaz M, Hussain A, Mahmood K, Imran M, Babar SAK (2017) Potential plant growth-promoting strain Bacillus sp. SR-2-1/1 decolorized azo dyes through NADH-ubiquinone: oxidoreductase activity. Bioresour Technol 235:176–184CrossRefGoogle Scholar
  35. Mao Y, Ai H, Chen Y, Zhang Z, Zeng P, Kang L, Li W, Gu W, He Q, Li H (2018) Phytoplankton response to polystyrene microplastics: perspective from an entire growth period. Chemosphere 208:59–68CrossRefGoogle Scholar
  36. Martorell MM, Pajot HF, Ahmed PM, de Figueroa LIC (2017) Biodecoloration of Reactive Black 5 by the methylotrophic yeast Candida boidinii MM 4035. J Environ Sci 53:78–87CrossRefGoogle Scholar
  37. Ng IS, Chen T, Lin R, Zhang X, Ni C, Sun D (2014) Decolorization of textile azo dye and Congo red by an isolated strain of the dissimilatory manganese-reducing bacterium Shewanella xiamenensis BC01. Appl Microbiol Biotechnol 98:2297–2308CrossRefGoogle Scholar
  38. Owabor CN, Ogbeide SE, Susu AA (2011) Growth kinetics of some subsurface microbial strains using naphthalene as a probe contaminant. Environ Technol 32:1453–1462CrossRefGoogle Scholar
  39. Paz A, Carballo J, Jose Perez M, Manuel Dominguez J (2017) Biological treatment of model dyes and textile wastewaters. Chemosphere 181:168–177CrossRefGoogle Scholar
  40. Qu W, Liu T, Wang D, Hong G, Zhao J (2018) Metagenomics-based discovery of malachite green-degradation gene families and enzymes from mangrove sediment. Front Microbiol 9:2187CrossRefGoogle Scholar
  41. Roucher A, Roussarie E, Gauvin RM, Rouhana J, Gounel S, Stines-Chaumeil C, Mano N, Backov R (2019) Bilirubin oxidase-based silica macrocellular robust catalyst for on line dyes degradation. Enzyme Microb Technol 120:77–83CrossRefGoogle Scholar
  42. Roy U, Sengupta S, Banerjee P, Das P, Bhowal A, Datta S (2018) Assessment on the decolourization of textile dye (Reactive Yellow) using Pseudomonas sp immobilized on fly ash: response surface methodology Chock for optimization and toxicity evaluation. J Environ Manag 223:185–195CrossRefGoogle Scholar
  43. Shah PD, Dave SR, Rao MS (2012) Enzymatic degradation of textile dye Reactive Orange 13 by newly isolated bacterial strain Alcaligenes faecalis PMS-1. Int Biodeterior Biodegrad 69:41–50CrossRefGoogle Scholar
  44. Shang N, Ding M, Dai M, Si H, Li S, Zhao G (2019) Biodegradation of malachite green by an endophytic bacterium Klebsiella aerogenes S27 involving a novel oxidoreductase. Appl Microbiol Biotechnol 103:2141–2153CrossRefGoogle Scholar
  45. Solis M, Solis A, Ines Perez H, Manjarrez N, Flores M (2012) Microbial decolouration of azo dyes: a review. Process Biochem 47:1723–1748CrossRefGoogle Scholar
  46. Tripathi A, Srivastava SK (2011) Ecofriendly treatment of azo dyes: biodecolorization using bacterial strains. Int J Biosci Biochem Bioinform 1:37–40Google Scholar
  47. Wang ZW, Liang JS, Liang Y (2013) Decolorization of Reactive Black 5 by a newly isolated bacterium Bacillus sp. YZU1. Int Biodeterior Biodegrad 76:41–48CrossRefGoogle Scholar
  48. Wang J-Z, Yue J-Y, Zhang C-H, Jia W, Li X, Sun Z (2016) Preparation of peptone from chicken bone residue by using natural pancreas as catalyst. J Chem Technol Biotechnol 91:2852–2861CrossRefGoogle Scholar
  49. Xie X, Liu N, Yang B, Yu C, Zhang Q, Zheng X, Xu L, Li R, Liu J (2016) Comparison of microbial community in hydrolysis acidification reactor depending on different structure dyes by Illumina MiSeq sequencing. Int Biodeterior Biodegrad 111:14–21CrossRefGoogle Scholar
  50. Xie X, Liu N, Ping J, Zhang Q, Zheng X, Liu J (2018) Illumina MiSeq sequencing reveals microbial community in HA process for dyeing wastewater treatment fed with different co-substrates. Chemosphere 201:578–585CrossRefGoogle Scholar
  51. Xie X, Zheng X, Yu C, Zhang Q, Wang Y, Cong J, Liu N, He Z, Yang B, Liu J (2019) Highly efficient biodegradation of reactive blue 19 under the activation of tea residue by a newly screened mixed bacterial flora DDMY2. RSC Adv 9:24791–24801CrossRefGoogle Scholar
  52. Yu C, Xie X, Zheng X, Xu L, Li R, Liu J (2016) Decolorization and repigmentation of reactive black 5 biodegradation and their mechanisms. Chem Ind Eng Prog 35:2987–2996Google Scholar
  53. Zhang Q, Xie X, Liu Y, Zheng X, Wang Y, Cong J, Yu C, Liu N, Liu J, Sand W (2019) Fructose as an additional co-metabolite promotes refractory dye degradation: performance and mechanism. Bioresour Technol 280:430–440CrossRefGoogle Scholar
  54. Zheng Q, Dai Y, Han X (2016) Decolorization of azo dye CI Reactive Black 5 by ozonation in aqueous solution: influencing factors, degradation products, reaction pathway and toxicity assessment. Water Sci Technol 73:1500–1510CrossRefGoogle Scholar
  55. Zheng X, Xie X, Yu C, Zhang Q, Wang Y, Cong J, Liu N, He Z, Yang B, Liu J (2019) Unveiling the activating mechanism of tea residue for boosting the biological decolorization performance of refractory dye. Chemosphere 233:110–119CrossRefGoogle Scholar
  56. Zhu Y, Cao H, Qiao S, Wang M, Gu Y, Luo H, Meng F, Liu X, Lai H (2015) Hierarchical micro/nanostructured titanium with balanced actions to bacterial and mammalian cells for dental implants. Int J Nanomed 10:6659–6674CrossRefGoogle Scholar

Copyright information

© Islamic Azad University (IAU) 2019

Authors and Affiliations

  • X. H. Xie
    • 1
    • 2
    • 3
    Email author
  • X. L. Zheng
    • 1
    • 2
  • C. Z. Yu
    • 1
    • 2
  • Q. Y. Zhang
    • 1
    • 2
  • Y. Q. Wang
    • 1
    • 2
  • J. H. Cong
    • 1
    • 2
  • N. Liu
    • 4
  • Z. J. He
    • 5
  • B. Yang
    • 1
    • 2
  • J. S. Liu
    • 1
    • 2
    • 3
  1. 1.College of Environmental Science and EngineeringDonghua UniversityShanghaiChina
  2. 2.State Environmental Protection Engineering Center for Pollution Treatment and Control in Textile IndustryDonghua UniversityShanghaiChina
  3. 3.Shanghai Institute of Pollution Control and Ecological SecurityShanghaiChina
  4. 4.School of Environment and Surveying EngineeringSuzhou UniversitySuzhouChina
  5. 5.School of Metallurgy and EnvironmentCentral South UniversityChangshaChina

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