Differential cell viability in Nitzschia palea on exposure to different organic and inorganic environmental effluents
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This research is focused to report the effects of various effluents on the cell viability and density of diatoms. Experiments have been performed on fresh water diatom Nitzschia palea with seven effluents, to check the cell viability and density using spectroscopic and microscopic techniques. On exposing the diatom cells to crude sewage water (40% and 60% dilution), there is approximately 10 times elevation in cell density without loss in the viability of cells, up to 25 days. On the other hand, individual exposure to β propranolol drugs resulted in a total loss of cell viability and cell destruction with only 10% of growth rate. Further, using sodium metasilicate as effluent, the diatom population dropped about 30%–60% on increasing concentration. Besides physiological stress, these effluents have also direct influence on the lipid production in diatoms. The objective of this research is to provide the monitoring report of different environmental effluents on water quality as well as their influence on lipid biosynthesis and also their impact over the diatom’s health which acts as a biological sensor for water ecosystem.
KeywordsDiatom Cell count Viability Lipid Environmental effluents
VV is thankful to DST Nanomission for sanctioning research project number GOI (SR/NM/NT/1090-2014) and INUP IIT Bombay project no Pid: P989423752/2016 to her and PS is thankful to UGC BSR project. MK is thankful to UGC for fellowship and SG to DST SERB for NPDF fellowships respectively. Authors thank state-of-the-art facility to use SEM at Dr Hari Singh Gour Central University, Sagar and FESEM and TEM facilities at INUP, IIT Bombay.
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