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Prospects of Stenotrophomonas pavanii DB1 in diesel utilization and reduction of its phytotoxicity on Vigna radiata

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Abstract

Diesel, for its carcinogenic and teratogenic properties, is considered as an environmental hazard. The present study concentrates on diesel utilization by Stenotrophomonas pavanii DB1 under various physicochemical parameters and mass spectral analysis of the metabolites produced. Soil remediation potential of the culture for reducing diesel toxicity against Vigna radiata seed germination was also evaluated. Supplementation of the broth with ammonium sulfate, molasses and tween 80 enhanced diesel utilization (up to 66.3%). A steady decrease in diesel utilization (69.46–51.23%) was recorded with the increase in salinity from 1 to 5000 mM. Similarly, the utilization efficiency was low (up to 11.5%) when cadmium, lead and mercury salts were present but was not affected by zinc and copper salts. Liquid chromatography–mass spectrometry revealed extensive utilization (95–99%) of both the short- (C10–C20) and long-chain (C18–C30) n-alkanes. Significantly, the culture exhibited uniform utilization of various recalcitrant n-alkanes, to generate metabolites like aldehyde, ketone, fatty acids, carboxylic and dicarboxylic acids. Stenotrophomonas pavanii DB1 mediated 95% and 86% of diesel utilization in soil with and without nutrient amendment, respectively. Germination percentage of V. radiata seeds improved with the bacterial inoculation of the diesel-containing soil. Stenotrophomonas pavanii DB1 culture did not establish antagonistic effect over the resident soil bacteria. Based on the spectral analysis and soil remediation studies, it could be inferred that S. pavanii DB1 is a potential culture for bioremediation of sites polluted with diesel.

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References

  1. Abou-Elela SI, Kamel MM, Fawzy ME (2010) Biological treatment of saline wastewater using a salt-tolerant microorganism. Desalination 250:1–5. https://doi.org/10.1016/j.desal.2009.03.022

  2. Agarry S, Latinwo GK (2015) Biodegradation of diesel oil in soil and its enhancement by application of bioventing and amendment with brewery waste effluents as biostimulation–bioaugmentation agents. J Ecol Eng 16:82–91. https://doi.org/10.12911/22998993/1861

  3. Al-Ghazawi Z, Saadoun I, Al-Shakah A (2005) Selection of bacteria and plant seeds for potential use in the remediation of diesel contaminated soils. J Basic Microbiol 45:251–256. https://doi.org/10.1002/jobm.200410503

  4. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410. https://doi.org/10.1016/S0022-2836(05)80360-2

  5. Antoniou E, Fodelianakis S, Korkakaki E, Kalogerakis N (2015) Biosurfactant production from marine hydrocarbon-degrading consortia and pure bacterial strains using crude oil as carbon source. Front Microbiol 6:274. https://doi.org/10.3389/fmicb.2015.00274

  6. Atagana HI, Haynes RJ, Wallis FM (2003) Optimization of soil physical and chemical conditions for the bioremediation of creosote-contaminated soil. Biodegradation 14:297–307. https://doi.org/10.1023/A:1024730722751

  7. Atlas RM, Cerniglia CE (1995) Bioremediation of petroleum pollutants—diversity and environmental aspects of hydrocarbon biodegradation. Bioscience 45:332–338. https://doi.org/10.2307/1312494

  8. Azubuike CC, Chikere CB, Okpokwasili GC (2016) Bioremediation techniques-classification based on site of application: principles, advantages, limitations and prospects. World J Microbiol Biotechnol 32:180. https://doi.org/10.1007/s11274-016-2137-x

  9. Baldrian P (2008) Wood-inhabiting ligninolytic basidiomycetes in soils: ecology and constraints for applicability in bioremediation. Fungal Ecol 1:4–12. https://doi.org/10.1016/j.funeco.2008.02.001

  10. Balseiro-Romero M, Gkorezis P, Kidd PS, Van Hamme J, Weyens N, Monterroso C, Vangronsveld J (2017) Characterization and degradation potential of diesel-degrading bacterial strains for application in bioremediation. Int J Phytoremediat 19:955–963. https://doi.org/10.1080/15226514.2017.1337065

  11. Bamforth SM, Singleton I (2005) Bioremediation of polycyclic aromatic hydrocarbons: current knowledge and future directions. J Chem Technol Biotechnol 80:723–736. https://doi.org/10.1002/jctb.1276

  12. Barathi S, Vasudevan N (2001) Utilization of petroleum hydrocarbons by Pseudomonas fluorescens isolated from a petroleum-contaminated soil. Environ Int 26:413–416. https://doi.org/10.1016/S0160-4120(01)00021-6

  13. Bartolucci S, Contursi P, Fiorentino G, Limauro D, Pedone E (2013) Responding to toxic compounds: a genomic and functional overview of Archaea. Front Biosci 18:165–189. https://doi.org/10.2741/4094

  14. Bhattacharya S, Das A, Prashanthi K, Palaniswamy M, Angayarkanni J (2014) Mycoremediation of benzo[a]pyrene by Pleurotus ostreatus in the presence of heavy metals and mediators. 3 Biotech 4:205–211. https://doi.org/10.1007/s13205-013-0148-y

  15. Bhattacharya S, Das A, Samadder S, Rajan SS (2016) Biosynthesis and characterization of a thermostable, alkali-tolerant chitinase from Bacillus pumilus JUBCH08 displaying antagonism against phytopathogenic Fusarium oxysporum. 3 Biotech 6:87. https://doi.org/10.1007/s13205-016-0406-x

  16. Cheng KY, Lai KM, Wong JW (2008) Effects of pig manure compost and non-ionic- surfactant Tween 80 on phenanthrene and pyrene removal from soil vegetated with Agropyron elongatum. Chemosphere 73:791–797. https://doi.org/10.1016/j.chemosphere.2008.06.005

  17. Crabtree HG (1928) The carbohydrate metabolism of certain pathological overgrowths. Biochem J 22:1289–1298. https://doi.org/10.1042/bj0221289

  18. Das N, Chandran P (2011) Microbial degradation of petroleum hydrocarbon contaminants: an overview. Biotechnol Res Int 2011:941810. https://doi.org/10.4061/2011/941810

  19. Daugulis AJ, McCracken RM (2003) Microbial degradation of high and low molecular weight polyaromatic hydrocarbons in a two-phase partitioning bioreactor by two strains of Sphingomonas sp. Biotechnol Lett 25:1441–1444. https://doi.org/10.1023/A:1025007729355

  20. Del’Arco JP, de Franca FP (2001) Influence of oil contamination levels on hydrocarbon biodegradation in sandy sediment. Environ Pollut 110:515–519. https://doi.org/10.1016/S0269-7491(00)00128-7

  21. Fathepure BZ (2014) Recent studies in microbial degradation of petroleum hydrocarbons in hypersaline environments. Front Microbiol 5:1–16. https://doi.org/10.3389/fmicb.2014.00173

  22. Gestel KV, Mergaert J, Swings J, Coosemans J, Ryckeboer J (2003) Bioremediation of diesel oil-contaminated soil by composting with biowaste. Environ Pollut 125:361–368. https://doi.org/10.1016/S0269-7491(03)00109-X

  23. Ghazali FM, Rahman RNZA, Salleh AB, Basri M (2004) Biodegradation of hydrocarbons in soil by microbial consortium. Int Biodeterior Biodegrad 54:61–67. https://doi.org/10.1016/j.ibiod.2004.02.002

  24. Kannan A, Upreti RK (2008) Influence of distillery effluent on germination and growth of mung bean (Vigna radiata) seeds. J Hazard Mater 153:609–615. https://doi.org/10.1016/j.jhazmat.2007.09.004

  25. Koeleman M, vd Laak WJ, Ietswaart H (1999) Dispersion of PAH and heavy metals along motorways in the Netherlands: an overview. Sci Total Environ 235:347–349. https://doi.org/10.1016/S0048-9697(99)00253-3

  26. Kostka JE, Prakash O, Overholt WA, Green SJ, Freyer G, Canion A, Delgardio J, Norton N, Hazen TC, Huettel M (2011) Hydrocarbon-degrading bacteria and the bacterial community response in Gulf of Mexico beach sands impacted by the Deepwater Horizon oil spill. Appl Environ Microbiol 77:7962–7974. https://doi.org/10.1128/AEM.05402-11

  27. Langbehn A, Steinhart H (1994) Determination of organic acids and ketones in contaminated soils. J High Resolut Chromatogr 17:293–298. https://doi.org/10.1002/jhrc.1240170502

  28. Leahy JG, Colwell RR (1990) Microbial degradation of hydrocarbons in the environment. Microbiol Rev 54:305–315

  29. Lin Y, Lay JJ, Shieh WK (2016) Diesel degradation in soil catalyzed by the addition of a bioagent. Int J Environ Sci Technol 13:551–560. https://doi.org/10.1007/s13762-015-0889-8

  30. Mccarthy K, Walker L, Vigoren L, Bartel J (2004) Remediation of spilled petroleum hydrocarbons by in situ landfarming at an Arctic Site. Cold Reg Sci Technol 40:31–39. https://doi.org/10.1016/j.coldregions.2004.05.001

  31. Mnif S, Sayadi S, Chamkha M (2014) Biodegradative potential and characterization of a novel aromatic-degrading bacterium isolated from a geothermal oil field under saline and thermophilic conditions. Int Biodeterior Biodegrad 86:258–264. https://doi.org/10.1016/j.ibiod.2013.09.015

  32. Movahedin H, Shokoohi R, Parvaresh A, Hajia M, Jafari AJ (2005) Evaluating the effect of glucose on phenol removal efficiency and changing the dominant microorganisms in a serial combined biological system. Pak J Biol Sci 8:1491–1494. https://scialert.net/abstract/?doi=pjbs.2005.1491.1494

  33. Nilesh PK, Hardik P (2013) Isolation and screening of hydrocarbon degrading bacteria from soil near Kadi (Gujarat) region. Int J Res BioSciences 2:10–16

  34. Olajire AA, Essien JP (2014) Aerobic degradation of petroleum components by microbial consortia. J Pet Environ Biotechnol 5:195. https://doi.org/10.4172/2157-7463.1000195

  35. Pacwa-Płociniczak M, Płaza GA, Poliwoda A, Piotrowska-Seget Z (2014) Characterization of hydrocarbon-degrading and biosurfactant-producing Pseudomonas sp. P-1 strain as a potential tool for bioremediation of petroleum-contaminated soil. Environ Sci Pollut Res Int 21:9385–9395. https://doi.org/10.1007/s11356-014-2872-1

  36. Palanisamy N, Ramya J, Kumar S, Vasanthi NS, Chandran P, Khan S (2014) Diesel biodegradation capacities of indigenous bacterial species isolated from diesel contaminated soil. J Environ Health Sci Eng 12:142. https://doi.org/10.1186/s40201-014-0142-2

  37. Rahman KS, Rahman TJ, Kourkoutas Y, Petsas I, Marchant R, Banat IM (2003) Enhanced bioremediation of n-alkane in petroleum sludge using bacterial consortium amended with rhamnolipid and micronutrients. Bioresour Technol 90:159–168. https://doi.org/10.1016/S0960-8524(03)00114-7

  38. Riis V, Babel W, Pucci OH (2002) Influence of heavy metals on the microbial degradation of diesel fuel. Chemosphere 49:559–568. https://doi.org/10.1016/S0045-6535(02)00386-7

  39. Rojo F (2009) Degradation of alkanes by bacteria. Environ Microbiol 11:2477–2490. https://doi.org/10.1111/j.1462-2920.2009.01948.x

  40. Shukor MY, Hassan NA, Jusoh AZ, Perumal N, Shamaan NA, MacCormack WP, Syed MA (2009) Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica. J Environ Biol 30:1–6

  41. Sutton NB, Langenhoff AA, Lasso DH, van der Zaan B, van Gaans P, Maphosa F, Smidt H, Grotenhuis T, Rijnaarts HH (2014) Recovery of microbial diversity and activity during bioremediation following chemical oxidation of diesel contaminated soils. Appl Microbiol Biotechnol 98:2751–2764. https://doi.org/10.1007/s00253-013-5256-4

  42. Valentín L, Feijoo G, Moreira MT, Lema JM (2006) Biodegradation of polycyclic aromatic hydrocarbons in forest and salt marsh soils by white-rot fungi. Int Biodeterior Biodegrad 58:15–21. https://doi.org/10.1016/j.ibiod.2006.04.002

  43. van Straalen NM (2002) Assessment of soil contamination: a functional perspective. Biodegradation 13:41–52. https://doi.org/10.1023/A:1016398018140

  44. Velho-Pereira S, Kamat NM (2011) Antimicrobial screening of actinobacteria using a modified cross-streak method. Indian J Pharm Sci 73:223–228. https://doi.org/10.4103/0250-474X.91566

  45. Vinothini C, Sudhakar S, Ravikumar R (2015) Biodegradation of petroleum and crude oil by Pseudomonas putida and Bacillus cereus. Int J Curr Microbiol App Sci 4:318–329

  46. Wentzel A, Ellingsen TE, Kotlar HK, Zotchev SB, Throne-Holst M (2007) Bacterial metabolism of long chain n-alkanes. Appl Microbiol Biotechnol 76:1209–1221. https://doi.org/10.1007/s00253-007-1119-1

  47. Ziagova M, Kyriakou G, Liakopoulou KM (2009) Co-metabolism of 2, 4-dichlorophenol and 4-Cl-m-cresol in the presence of glucose as an easily assimilated carbon source by Staphylococcus xylosus. J Hazard Mater 163:383–390. https://doi.org/10.1016/j.jhazmat.2008.06.102

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Acknowledgments

We extend our sincere gratitude to the management of JAIN (Deemed-to-be University) for providing the research facilities.

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Correspondence to S. Bhattacharya.

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The authors declare that they have no conflict of interest.

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Editorial responsibility: M. Abbaspour.

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Bhattacharya, S., Das, A., Srividya, S. et al. Prospects of Stenotrophomonas pavanii DB1 in diesel utilization and reduction of its phytotoxicity on Vigna radiata. Int. J. Environ. Sci. Technol. 17, 445–454 (2020). https://doi.org/10.1007/s13762-019-02302-w

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Keywords

  • n-alkane
  • Diesel utilization
  • Seed germination
  • Stenotrophomonas pavanii DB1
  • Vigna radiata