Bioleaching of heavy metals from spent batteries using Aspergillus nomius JAMK1

  • A. Chatterjee
  • R. Das
  • J. AbrahamEmail author
Original Paper


The present study focuses on recovery of heavy metals from e-waste fungus isolated from heavy metal rich industrial sewage soil. Molecular identification revealed the organism to be Aspergillus nomius and was designated as A. nomius JAMK1. The isolate was subjected for heavy metals uptake following two mechanisms: bioaccumulation and biosorption. Atomic absorption spectroscopy (AAS) was primarily used to analyse the heavy metal ions uptake and further confirmed by scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDAX). Kinetics study was performed using pseudo-first-order, pseudo-second-order, Elovich, Weber and Morris and Boyd’s kinetic model. Central composite design, a subunit of response surface methodology, was utilized to obtain design of experiments for the experimental values in bioaccumulation. Adsorption isotherm assessment, conducted for the biosorption activity of strain JAMK1, showed that the process followed Freundlich mode of isotherm. The adsorbed heavy metals were comprehensively desorbed from the metal-loaded sorbent using HCl and Ca(OH)2. The activity of A. nomius JAMK1 in bioleaching of metals from spent batteries was studied and analysed using AAS, X-ray diffraction, SEM and EDAX. The investigation proved A. nomius JAMK1 to be a potential strain for heavy metals uptake from aqueous solution as well as electronic waste.


Bioaccumulation Biosorption Desorbed Electronic waste Kinetics study 



The authors would like to express their gratitude to the management of VIT, Vellore.

Compliance with ethical standards

Conflict of interest

The authors have no conflict of interest to declare.


  1. Abdel-Aty AM, Ammar NS, Ghafar HH, Ali RK (2013) Biosorption of cadmium and lead from aqueous solution by fresh water alga Anabaena sphaerica biomass. J Adv Res 4(4):367–374. Google Scholar
  2. Acharya J, Sahu JN, Mohanty CR, Meikap BC (2009) Removal of lead(II) from wastewater by activated carbon developed from tamarind wood by zinc chloride activation. Chem Eng J 149(1–3):249–262Google Scholar
  3. Ahmad I, Zafar S, Ahmad F (2005) Heavy metal biosorption potential of Aspergillus and Rhizopus sp. isolated from wastewater treated soil. J Appl Sci Environ Manage 9(1):123–126Google Scholar
  4. Ajenifuja E, Ajao JA, Ajayi EO (2017) Equilibrium adsorption isotherm studies of Cu(II) and Co(II) in high concentration aqueous solutions on Ag–TiO2-modified kaolinite ceramic adsorbents. Appl Water Sci 7(5):2279–2286. Google Scholar
  5. Al-Garni SM (2005) Biosorption of lead by Gram-ve capsulated and non-capsulated bacteria. Water SA 31(3):345–350Google Scholar
  6. Anand P, Isar J, Saran S, Saxena RK (2006) Bioaccumulation of copper by Trichoderma viride. Bioresour Technol 97(8):1018–1025. Google Scholar
  7. Arshadi M, Amiri MJ, Mousavi S (2014) Kinetic, equilibrium and thermodynamic investigations of Ni(II), Cd(II), Cu(II) and Co(II) adsorption on barley straw ash. Water Resour Ind 6:1–17. Google Scholar
  8. Baldrian P (2003) Interactions of heavy metals with white-rot fungi. Enzyme Microb Technol 32(1):78–91. Google Scholar
  9. Barakat MA (2011) New trends in removing heavy metals from industrial wastewater. Arab J Chem 4(4):361–377. Google Scholar
  10. Barkakati P, Begum A, Das ML, Rao PG (2010) Adsorptive separation of Ginsenoside from aqueous solution by polymeric resins: equilibrium, kinetic and thermodynamic studies. Chem Eng J 161(1–2):34–45Google Scholar
  11. Bhuvaneshwari S, Sruthi D, Sivasubramanian V, Kanthimathy K (2012) Regeneration of chitosan after heavy metal sorption. J Sci Ind Res India 71:266–269Google Scholar
  12. Borthakur A, Sinha K (2013) Generation of electronic waste in India: current scenario, dilemmas and stakeholders. Afr J Environ Sci Technol 7(9):899–910. Google Scholar
  13. Çeribasi IH, Yetis U (2001) Biosorption of Ni(II) and Pb(II) by Phanerochaete chrysosporium from a binary metal system–kinetics. Water SA 27(1):15–20. Google Scholar
  14. Chang SH, Teng TT, Ismail N (2011) Optimization of Cu(II) extraction from aqueous solutions by soybean-oil-based organic solvent using response surface methodology. Water Air Soil Pollut 217(1–4):567–576. Google Scholar
  15. Chatterjee A, Abraham J (2017) Efficient management of E-wastes. Int J Environ Sci Technol 14(1):211–222. Google Scholar
  16. Ciesielczyk F, Bartczak P, Jesionowski T (2016) Removal of cadmium(II) and lead(II) ions from model aqueous solutions using sol–gel-derived inorganic oxide adsorbent. Adsorption 22(4–6):445–458. Google Scholar
  17. Das S (2012) Biosorption of chromium and nickel by dried biomass of Cyanobacterium Oscillatoria laete-virens. Int J Environ Sci 3(1):341–352. Google Scholar
  18. Dezam AP, Vasconcellos VM, Lacava PT, Farinas CS (2017) Microbial production of organic acids by endophytic fungi. Biocatal Agric Biotechnol 11:282–287. Google Scholar
  19. Dursun AY, Uslu G, Cuci Y, Aksu Z (2003) Bioaccumulation of copper(II), lead(II) and chromium(VI) by growing Aspergillus niger. Process Biochem 38(12):1647–1651. Google Scholar
  20. Dwivedi S, Mishra A, Saini D (2012) Removal of heavy metals in liquid media through fungi isolated from wastewater. Int J Sci Res 1(3):181–185Google Scholar
  21. Flouty R, Estephane G (2012) Bioaccumulation and biosorption of copper and lead by a unicellular algae Chlamydomonas reinhardtii in single and binary metal systems: a comparative study. J Environ Manage 111:106–114. Google Scholar
  22. Gajendiran A, Abraham J (2017) Biomineralisation of fipronil and its major metabolite, fipronil sulfone, by Aspergillus glaucus strain AJAG1 with enzymes studies and bioformulation. 3 Biotech 7(3):212. Google Scholar
  23. Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol Ecol 2(2):113–118. Google Scholar
  24. Gupta P, Kumar P, Singh N (2015) Adsorption of copper metal by living Aspergillus niger L. biomass. Int J Environ Sci 5(6):1122–1133. Google Scholar
  25. Hasan SH, Srivastava P, Talat M (2009) Biosorption of Pb(II) from water using biomass of Aeromonas hydrophila: central composite design for optimization of process variables. J Hazard Mater 168(2–3):1155–1162. Google Scholar
  26. Horeh NB, Mousavi SM, Shojaosadati SA (2016) Bioleaching of valuable metals from spent lithium-ion mobile phone batteries using Aspergillus niger. J Power Sources 320:257–266Google Scholar
  27. Hussain A, Hamayun M, Rahman H, Iqbal A, Shah M, Irshad M, Qasim M, Islam B (2018) Bioremediation of hexavalent chromium by endophytic fungi; safe and improved production of Lactuca sativa L. Chemosphere 211:653–663. Google Scholar
  28. Husaini A, Fisol FA, Yun LC, Hussain MH, Roslan HA (2011) Lignocellulolytic enzymes produced by tropical white rot fungi during biopulping of Acacia mangium wood chips. J Biochem Technol 3:245–250Google Scholar
  29. Iram S, Abrar S (2015) Biosorption of copper and lead by heavy metal resistant fungal isolates. Int J Sci Res Publ 5(1):1–5Google Scholar
  30. Jianlong W, Xinmin Z, Decai D, Ding Z (2001) Bioadsorption of lead(II) from aqueous solution by fungal biomass of Aspergillus niger. J Biotechnol 87(3):273–277. Google Scholar
  31. Joo JH, Hussein KA (2012) Heavy metal tolerance of fungi isolated from contaminated soil. KJSSF 45(4):565–571. Google Scholar
  32. Joshi NC (2017) Heavy metals, conventional methods for heavy metal removal, biosorption and the development of low cost adsorbent. Eur J Pharm Med Res 4:388–393Google Scholar
  33. Joshi PK, Swarup A, Maheshwari S, Kumar R, Singh N (2011) Bioremediation of heavy metals in liquid media through fungi isolated from contaminated sources. Indian J Microbiol 51(4):482–487. Google Scholar
  34. Kariuki Z, Kiptoo J, Onyancha D (2017) Biosorption studies of lead and copper using rogers mushroom biomass ‘Lepiota hystrix’. S Afr J Chem Eng 23:62–70. Google Scholar
  35. Khalid MA (2017) Bioaccumulation of chromium and nickel by fungal isolates from tannery effluent collection site from Kanpur, Uttar Pradesh, India. IJGP 11(03):S604–S609. Google Scholar
  36. Kim JO, Lee YW, Chung J (2013) The role of organic acids in the mobilization of heavy metals from soil. KSCE J Civ Eng 17(7):1596–1602. Google Scholar
  37. Kumar PS, Vincent C, Kirthika K, Kumar KS (2010) Kinetics and equilibrium studies of Pb2+ in removal from aqueous solutions by use of nano-silversol-coated activated carbon. Braz J Chem Eng 27(2):339–346. Google Scholar
  38. Li QZ, Chai LY, Jing ZH, Yang ZH, Wang QW (2009) Lead desorption from modified spent grain. T Nonferr Metal Soc 19(5):1371–1376. Google Scholar
  39. Lim SF, Lee AY (2015) Kinetic study on removal of heavy metal ions from aqueous solution by using soil. Environ Sci Pollut Res 22(13):10144–10158. Google Scholar
  40. Lo W, Ng LM, Chua H, Peter HF, Sin SN, Wong PK (2003) Biosorption and desorption of copper(II) ions by Bacillus sp. In: Davidson BH et al (eds) Biotechnology for fuels and chemicals. Humana Press, Totowa, NJ, pp 581–591Google Scholar
  41. Mishra A, Malik A (2012) Simultaneous bioaccumulation of multiple metals from electroplating effluent using Aspergillus lentulus. Water Res 46(16):4991–4998. Google Scholar
  42. More SS, PS R, Malini S (2011) Isolation, purification, and characterization of fungal laccase from Pleurotus sp. Enzyme Res 2011:2011. Google Scholar
  43. Niu Z, Zou Y, Xin B, Chen S, Liu C, Li Y (2014) Process controls for improving bioleaching performance of both Li and Co from spent lithium ion batteries at high pulp density and its thermodynamics and kinetics exploration. Chemosphere 104:92–98. Google Scholar
  44. Oladipo OG, Awotoye OO, Olayinka A, Bezuidenhout CC, Maboeta MS (2018) Heavy metal tolerance traits of filamentous fungi isolated from gold and gemstone mining sites. Braz J Microbiol 49(1):29–37. Google Scholar
  45. Pandit V (2016) India’s e-waste growing at 30% annually. The Hindu BusinessLine. Accessed 29 Sep 2018
  46. Perkins DN, Drisse MN, Nxele T, Sly PD (2014) E-waste: a global hazard. Ann Glob Health 80(4):286–295. Google Scholar
  47. Rao PR, Bhargavi C (2013) Studies on biosorption of heavy metals using pretreated biomass of fungal species. Int J Chem Chem Eng 3(3):171–180Google Scholar
  48. Rashid M, Khalil S, Ayub N, Alam S, Latif F (2004) Organic acids production and phosphate solubilization by phosphate solubilizing microorganisms (PSM) under in vitro conditions. Pak J Biol Sci 7(2):187–196Google Scholar
  49. Renu B, Agarwal M, Singh K (2017) Methodologies for removal of heavy metal ions from wastewater: an overview. IER 18(2):124–142. Google Scholar
  50. Saeed A, Akhter MW, Iqbal M (2005) Removal and recovery of heavy metals from aqueous solution using papaya wood as a new biosorbent. Sep Purif Technol 45(1):25–31. Google Scholar
  51. Say R, Denizli A, Arıca MY (2001) Biosorption of cadmium(II), lead(II) and copper(II) with the filamentous fungus Phanerochaete chrysosporium. Bioresource Technol 76(1):67–70. Google Scholar
  52. Secretariat RS (2011) E-waste in India. India Research Unit (Larrdis), Rajya Sabha Secretariat, New Delhi. Accessed on 25 May 2018
  53. Shaikh Z, Qureshi P (2013) Screening and isolation of organic acid producers from samples of diverse habitats. Int J Curr Microbiol Appl Sci 2(9):39–44Google Scholar
  54. Sharma S, Tiwari S, Hasan A, Saxena V, Pandey LM (2018) Recent advances in conventional and contemporary methods for remediation of heavy metal-contaminated soils. 3 Biotech 8(4):216. Google Scholar
  55. Shekher R, Sehgal S, Kamthania M, Kumar A (2011) Laccase: microbial sources, production, purification, and potential biotechnological applications. Enzyme Res 2011:217861. Google Scholar
  56. Siddiquee S, Rovina K, Azad SA, Naher L, Suryani S, Chaikaew P (2015) Heavy metal contaminants removal from wastewater using the potential filamentous fungi biomass: a review. J Microb Biochem Technol 7(6):384–395. Google Scholar
  57. Siham AK (2007) Effect of lead and copper on the growth of heavy metal resistance fungi isolated from second industrial city in riyadh. Saudi Arab J Appl Sci 7:1019–1024. Google Scholar
  58. Silambarasan S, Abraham J (2013) Ecofriendly method for bioremediation of chlorpyrifos from agricultural soil by novel fungus Aspergillus terreus JAS1. Water Air Soil Pollut 224(1):1369–1379. Google Scholar
  59. Singh KK, Hasan SH, Talat M, Singh VK, Gangwar SK (2009) Removal of Cr (VI) from aqueous solutions using wheat bran. Chem Eng J 151(1–3):113–121. Google Scholar
  60. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24(8):1596–1599. Google Scholar
  61. Tran HN, Chao HP (2018) Adsorption and desorption of potentially toxic metals on modified biosorbents through new green grafting process. Environ Sci Pollut R. Google Scholar
  62. Wierzba S, Latała A (2010) Biosorption lead(II) and nikel(II) from an aqueous solution by bacterial biomass. Pol J Chem Technol 12(3):72–78. Google Scholar
  63. Witek-Krowiak A (2013) Application of beech sawdust for removal of heavy metals from water: biosorption and desorption studies. Eur J Wood Wood Prod 71(2):227–236. Google Scholar
  64. Zahoor M, Irshad M, Rahman H, Qasim M, Afridi SG, Qadir M, Hussain A (2017) Alleviation of heavy metal toxicity and phytostimulation of Brassica campestris L. by endophytic Mucor sp. MHR-7. Ecotoxicol Environ Saf 142:139–149. Google Scholar

Copyright information

© Islamic Azad University (IAU) 2019

Authors and Affiliations

  1. 1.Microbial Biotechnology Laboratory, School of Biosciences and TechnologyVIT UniversityVelloreIndia
  2. 2.Department of Mathematics, School of Advanced SciencesVIT UniversityVelloreIndia

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