Biogreen remediation of chromium-contaminated soil using Pseudomonas sp. (RPT) and neem (Azadirachta indica) oil cake

  • M. Govarthanan
  • T. Selvankumar
  • R. Mythili
  • P. Srinivasan
  • F. Ameen
  • S. A. AlYahya
  • S. Kamala-KannanEmail author
Original Paper


The prospective of indigenous Pseudomonas sp. (RPT) and neem oil cake for enhanced removal of chromium (Cr) from contaminated soil microcosm was explored in this study. The bacteria were isolated from Cr-contaminated soil and identified as Pseudomonas sp. based on partial 16S rDNA sequencing. The isolate RPT showed high Cr(VI) tolerance (1000 mg/l) and removal rate (64.4%) in batch experiments. Transmission electron microscopy observation showed that the isolate effectively precipitated the Cr both intra- and extracellularly. Microcosm studies revealed that neem oil cake amendment (7.5% w/v) enhanced Cr(VI) removal (82%) from contaminated soil. Furthermore, soil enzyme activities were increased in the biostimulated soil. The obtained results indicated that the application of neem oil cake along with indigenous Cr(VI)-resistant bacteria could inspire the bioremediation of Cr(VI)-contaminated soil field scale.


Chromium Neem oil cake Pseudomonas sp. Remediation Soil 



This paper was supported by research funds of Chonbuk National University in 2017. The authors (Fuad Ameen & Sami A. AlYahya) are very grateful to the Localization and Development Technology Platform for the Infectious Diseases Surveillance and the Detection Project at Kind Abdulaziz City for Science and Technology.

Compliance with ethical standards

Conflict of interest

The authors declare that they do not have any conflict of interest.


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Copyright information

© Islamic Azad University (IAU) 2018

Authors and Affiliations

  1. 1.Department of Environmental EngineeringUniversity of SeoulSeoulSouth Korea
  2. 2.PG and Research Department of BiotechnologyMahendra Arts and Science College (Autonomous)Kalippatti, NamakkalIndia
  3. 3.Department of Botany and Microbiology, College of ScienceKing Saud UniversityRiyadhSaudi Arabia
  4. 4.National Centre for BiotechnologyKing Abdulaziz City for Science and Technology (KACST)RiyadhSaudi Arabia
  5. 5.Division of Biotechnology, College of Environmental and Bioresource SciencesChonbuk National UniversityIksanSouth Korea

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