Bioconversion of toxic micropollutant triclosan to 2,4-dichlorophenol using a wastewater isolate Pseudomonas aeruginosa KS2002

  • R. Kumari
  • S. Ghosh SachanEmail author
Original Paper


Triclosan, a commonly available pesticide, has emerged as a ubiquitous pollutant posing a major threat to the environment. Here we have isolated a wastewater microorganism, Pseudomonas aeruginosa KS2002, capable of converting triclosan to 2,4-dichlorophenol within 96 h of incubation. The confirmation of the end product was done using Fourier transform infrared spectroscopy and mass spectroscopy. Different minimal media were investigated to establish a suitable media supporting maximum triclosan degradation. Spectral analysis showed that this bacterial isolate degraded 99.89% ± 0.3 of 2 g/L of triclosan spiked in an M9 minimal salt medium. This isolate utilized fructose and glycerol as a co-substrate to enhance degradation process. The cell-free extract of Pseudomonas aeruginosa KS2002 showed the activity of catechol 2,3-dioxygenase enzyme (specific enzyme activity = 0.161 U/mg). In the presence of 3-fluorocatechol, a meta-cleavage enzyme inhibitor, triclosan degradation was ceased suggesting a meta-cleavage pathway for triclosan degradation. Keeping in view the observations recorded, we proposed a pathway for partial triclosan degradation using this isolate.


Catechol 2,3-dioxygenase 3-fluorocatechol Mass spectroscopy Meta-cleavage 



The authors are extremely thankful to the Council of Scientific and Industrial Research (Scheme No. 24(0340)/16/EMR-II) for providing financial assistance for the research work. We would also like to acknowledge Department of Bio-Engineering and Central Instrumentation Facility (CIF) at Birla Institute of Technology, Mesra, for providing us with the infrastructure to conduct our research work.

Compliance with ethical standards

Conflict of Interests

The authors declare that they have no conflict of interest.


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Copyright information

© Islamic Azad University (IAU) 2018

Authors and Affiliations

  1. 1.Department of Bio-EngineeringBirla Institute of Technology, MesraRanchiIndia

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