Salinity-induced oxidative stress-mediated change in fatty acids composition of cyanobacterium Synechococcus sp. PCC7942

  • E. Verma
  • S. Singh
  • Niveshika
  • A. K. MishraEmail author
Original Paper


The present study was undertaken to examine the salinity stress-induced physiological and biochemical alterations in the cyanobacterium Synechococcus sp. PCC 7942. Cyanobacterial cultures supplemented with different concentrations of NaCl were evaluated for growth, carbohydrate, total lipid, ROS generation, and stress biomarkers to evaluate the ROS-mediated lipid production in Synechococcus 7942. Salt concentration of 500 mM induced a five- and threefold increase in the production of carbohydrates and lipids, respectively. The fatty acids composition in terms of total quantity and oleic acid content of the investigated species was also improved as the salinity level increased from 0 to 500 mM NaCl. The data showed maximum MUFA production at 10 mM NaCl with dominance of palmitoleic acid (88.3%) and oleic acid (0.31%), whereas PUFA was found to be maximally produced at 250 mM NaCl with dominance of linoleic acid. Salt stress enhanced the accumulation of carbohydrate and total lipids and antioxidative enzymes, and modulates the fatty acids and hydrocarbon composition of cyanobacterium. Production of fatty acid and hydrocarbon under saline conditions indicates that salinity can be used as a factor to modulate the biochemical pathways of cyanobacteria toward efficient biofuel production.


Biofuel FAMEs Lipids Hydrocarbons Salinity Synechococcus sp. PCC 7942 



Ascorbate peroxidase




Cyclic hydrocarbon


Fatty acid methyl esters


Gas chromatography/mass spectrometry




Monounsaturated fatty acid


Polyunsaturated fatty acid


Reactive oxygen species


Saturated fatty acid


Saturated hydrocarbon


Superoxide dismutase


Unsaturated hydrocarbon



We are thankful to the Head, Department of Botany, Banaras Hindu University, Varanasi, India, for providing laboratory facilities. We thank Prof. Karl Forchhammer, Department of Organismic Interactions (Microbiology), Interfaculty Institute of Microbiology and Infection, Auf der Morgenstelle, 2872076, University of Tübingen, Germany, for providing Synechococcus sp. PCC 7942 strain. Two of us (Ekta Verma and Niveshika) are thankful to the UGC, New Delhi, for financial support in the form of JRF.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13762_2018_1720_MOESM1_ESM.tif (643 kb)
Fig. S1. Growth of the cyanobacterium Synechococcus sp. in terms of dry weight (TIFF 642 kb)
13762_2018_1720_MOESM2_ESM.tif (625 kb)
Figure S2 DCF fluorescence-based G/R ratio of Synechococcus sp. obtained from fluorescence microscopic analysis under NaCl stress (TIFF 625 kb)
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Figure S3. GC/MS Chromatograms of control, 10, 50, 100, 250 and 500 mM NaCl-treated cyanobacterial cells (Fig. 1a, 1b, 1c, 1d, 1e, 1f, respectively) (EPS 3134 kb)
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Supplementary material 4 (EPS 3137 kb)
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Supplementary material 5 (EPS 3135 kb)
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Supplementary material 6 (EPS 3130 kb)
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Supplementary material 7 (EPS 3130 kb)
13762_2018_1720_MOESM8_ESM.eps (3.1 mb)
Supplementary material 8 (EPS 3133 kb)
13762_2018_1720_MOESM9_ESM.docx (663 kb)
Supplementary material 9 (DOCX 663 kb)
13762_2018_1720_MOESM10_ESM.docx (580 kb)
Supplementary material 10 (DOCX 580 kb)


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Copyright information

© Islamic Azad University (IAU) 2018

Authors and Affiliations

  1. 1.Laboratory of Microbial Genetics, Department of BotanyBanaras Hindu UniversityVaranasiIndia

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