Copper effect in petroleum hydrocarbons biodegradation by microorganisms associated to Juncus maritimus: role of autochthonous bioaugmentation
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The bioremediation potential of microorganisms from a saltmarsh plant rhizosphere and application of bioaugmentation in estuarine sediment co-contaminated were investigated. Rhizosediment (sediment in contact with plant roots) of Juncus maritimus was contaminated with copper and/or petroleum, inoculated with different autochthonous microbial consortia (resistant to copper and/or with petroleum degraders) and put in vessels to which plants were transplanted. Vessels were irrigated through a system that simulated estuarine tides. After 5 months, vessels were dismantled and copper and petroleum content in rhizosediments were determined. Copper’s presence reduced the potential of the microorganisms associated to J. maritimus rhizosphere for bioremediation of petroleum hydrocarbons in co-contaminated sediment. Indeed, hydrocarbons removal decreased from 39 to 25% when copper was present. In addition, bioaugmentation was not effective to overcome metal negative effects on petroleum hydrocarbons degradation, and the same removal rate was being observed (ca. 25%). Different methodologies for the formulation of consortia must be tested in this situation of co-contamination. Obtained results should be taken in consideration when planning the recovery of moderately impacted estuaries, aiming an effective protection and management of these areas, in the case of co-contamination.
KeywordsBioremediation Co-contaminated soil Estuarine environment Saltmarsh plants
To Rayra Santiago, Tânia Oliveira, Tatiana Necrasov, Catarina Magalhães for their help in the experiments assembling and dismantling of the vessels. This research was partially supported by the Strategic Funding UID/Multi/04423/2013 through national funds provided by FCT—Foundation for Science and Technology and European Regional Development Fund (ERDF), in the framework of the programme PT2020 and by the structured Program of R&D&I INNOVMAR—Innovation and Sustainability in the Management and Exploitation of Marine Resources, reference NORTE-01-0145-FEDER-000035, namely within the Research Line ECOSERVICES (Assessing the environmental quality, vulnerability and risks for the sustainable management of the NW coast natural resources and ecosystem services in a changing world) within the R&D Institution CIIMAR (Interdisciplinary Centre of Marine and Environmental Research), supported by the Northern Regional Operational Programme (NORTE2020), through the European Regional Development Fund (ERDF).
- Akpoveta OV, Egharevba F, Medjor GW (2011) A pilot study on the biodegradation of hydrocarbon and its kinetics on kerosene simulated soil. Intern J Environ Sci 2:54–67Google Scholar
- Almeida CMR, Couto N, Ribeiro H, Mucha AP, Bordalo A, Basto MC, Vasconcelos MTSD (2015) Salt marsh plants’ potential for the remediation of hydrocarbons-contaminated environments. In: Ansari AA, Gill SS, Gill R, Lanza GR, Newman L (eds) Phytoremediation: management of environmental contaminants, vol 1. Springer, Berlin, pp 323–331. doi: 10.1007/978-3-319-10395-2_23 Google Scholar
- Colombo M, Cavalca L, Bernasconi S, Andreoni V (2011) Bioremediation of polyaromatic hydrocarbon contaminated soils by microflora and bioaugmentation with Sphingobium chlorophenolicum strain C3R: a feasibility study in solid- and slurry-phase microcosms. Int Biodeter Biodegr 65:191–197CrossRefGoogle Scholar
- Couto MN, Borges JR, Guedes P, Almeida R, Monteiro E, Almeida CMR, Basto MCP, Vasconcelos MTSD (2014) An improved method for determination of petroleum hydrocarbons from soil using a simple ultrasonic extraction and Fourier transform infrared spectrophotometry. Pet Sci Technol 32:426–432CrossRefGoogle Scholar
- Lorah MM, Majcher EH, Jones EJ, Voytek MA (2008) Microbial consortia development and microcosm and column experiments for enhanced bioremediation of chlorinated volatile organic compounds, west branch canal creek wetland area, Aberdeen Proving Ground, Maryland, U.S. Geological Survey Scientific Investigations Report 2007-5165. http://md.water.usgs.gov/publications/sir.html
- Rani R, Juwarkar A (2013) Interactions between plant growth promoting microbes and plants: implications for microbe-assisted phytoremediation of metal-contaminated soil. In: Leung DWM (ed) Recent advances towards improved phytoremediation of heavy metal pollution. Bentham Science Publishers, Sharjah, pp 3–39CrossRefGoogle Scholar
- Said WA, Lewis DL (1991) Quantitative assessment of the effects of metals on microbial degradation of organic chemicals. Appl Environ Microb 57:1498–1503Google Scholar
- Sprocati AR, Alisi C, Tasso F, Marconi P, Sciullo A, Pinto V, Chiavarini S, Ubaldi C, Cremisini C (2012) Effectiveness of a microbial formula, as a biaugmentation agent, tailored for bioremediation of diesel oil and heavy metal co-contaminated soil. Process Biochem 47:1649–1655CrossRefGoogle Scholar
- Wang K, Zhu Z, Huang H, Li T, He Z, Yang X, Alva A (2012) Interactive effects of Cd and PAHs on contaminants removal from co-contaminated soil planted with hyperaccumulator plant Sedum alfredii. J Soils Sedim 12:554–564Google Scholar
- Weyens N, Truyens S, Saenen E, Boulet J, Dupae J, Taghavi S, van der Lelie D, Carleer R, Vangronsveld J (2011) Endophytes and their potential to deal with co-contamination of organic contaminants (Toluene) and toxic metals (Nickel) during phytoremediation. Int J Phytoremediat 13:244–255CrossRefGoogle Scholar