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Bioremediation of crude oil by Bacillus licheniformis in the presence of different concentration nanoparticles and produced biosurfactant

  • H. S. El-SheshtawyEmail author
  • W. Ahmed
Original Paper

Abstract

This research work focuses on testing the bacterial strain Bacillus licheniformis for the bioremediation capacity of the crude oil. A biosurfactant and two different nanoparticles with different concentrations (0.05, 0.1, 0.2 g/100 ml) were applied separately to enhance the biodegradation process. The optimum biodegradation of crude oil was demonstrated at 60% of microcosms containing biosurfactant and nanoparticles after 7 days. The bacterial strain is highly potential to consume the total paraffins (iso- and n-paraffins) in crude oil samples. Accordingly, the best biodegradation of total paraffins was observed in microcosms containing (0.2 g) of Fe2O3, Zn5(OH)8Cl2 (nps) and biosurfactant separately. Additionally, the consumption of specific member rings of polyaromatics depends on the type and the concentration of nanoparticles. Thus, this bacterial strain was considered as a good candidate to be applied in the bioremediation process of petroleum-contaminated sites using biosurfactant and specific concentration of (Fe2O3 and Zn5OH8Cl2) nanoparticles.

Keywords

Bacillus licheniformis Bioremediation Biosurfactants Nanoparticles Crude oil 

Notes

Acknowledgements

This work was sponsored by the Egyptian Petroleum Research Institute (EPRI), Cairo, Egypt. The author would like to extend thanks to all members of the Application of Biotechnology in the Field of Petroleum Industry Lab, Process, Design and Development Department (EPRI).

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Copyright information

© Islamic Azad University (IAU) 2017

Authors and Affiliations

  1. 1.Egyptian Petroleum Research Institute (EPRI)CairoEgypt

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