Two-phase anaerobic co-digestion of used vegetable oils’ wastes and pig manure

  • D. Hidalgo
  • M. Gómez
  • J. M. Martín-Marroquín
  • A. Aguado
  • E. Sastre
Original Paper


The purpose of this work was to study the anaerobic co-digestion of all the by-products generated during the processing of used vegetable oils (UVO) with the objective of proposing a solution for the treatment of these wastes. More specifically, this study investigates the advantages of two-phase anaerobic digestion for treating a mixture (1/5 v/v) of UVO processing wastes (OW) and pig manure (PM) using two semi-continuous digesters operated at mesophilic temperature (37 ± 1 °C). The experiments were conducted at hydraulic retention time (HRT) of 0.5, 1, 2 and 4 days in the first stage (acidifier) and at HRT of 11.5, 15, 18 and 20 days in the second stage (methaniser). The results revealed that the HRT had a high influence on the soluble chemical oxygen demand (sCOD) and total dissolved solids (TDS) removal. The maximum total sCOD removal efficiency of 86.4 % and TDS removal efficiency of 81.9 % was achieved at 20 days of global HRT. The maximum biogas production of 0.65 m3 per kilogramme of volatile dissolved solids (VDS) was removed, corresponding to a methane production of 0.42 m3 CH4 kg−1. VDS removed (65 % CH4) was also achieved at 20 days of HRT. The two-phase digestion system showed good stability, which was mainly attributed to the strong buffering capacity with the two-phase system and the high alkalinity from PM when co-digested with OW. The results obtained from this study provide fundamental information for scaling up a high-performance two-phase anaerobic system in the future.


Co-digestion Hydraulic retention time Oily waste Pig manure Two-phase anaerobic reactor Used vegetable oil 



The authors gratefully acknowledge support of this work by the LIFE + Program under the responsibility of the Directorate General for the Environment of the European Commission through the agreement LIFE 09 ENV/E/000451-VALUVOIL project.


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Copyright information

© Islamic Azad University (IAU) 2014

Authors and Affiliations

  • D. Hidalgo
    • 1
    • 2
  • M. Gómez
    • 1
  • J. M. Martín-Marroquín
    • 1
    • 2
  • A. Aguado
    • 1
    • 2
  • E. Sastre
    • 1
  1. 1.CARTIF Centro TecnológicoValladolidSpain
  2. 2.ITAP InstituteUniversity of ValladolidValladolidSpain

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