Bacterial community change through drinking water treatment processes

  • X. Liao
  • C. Chen
  • Z. Wang
  • C.-H. Chang
  • X. Zhang
  • S. Xie
Original Paper

Abstract

The microbiological quality of drinking water has aroused increasing attention due to potential public health risks. Knowledge of the bacterial ecology in the effluents of drinking water treatment units will be of practical importance. However, the bacterial community in the effluents of drinking water filters remains poorly understood. The changes of the density of viable heterotrophic bacteria and bacterial populations through a pilot-scale drinking water treatment process were investigated using heterotrophic plate counts and clone library analysis, respectively. The pilot-scale treatment process was composed of preozonation, rapid mixing, flocculation, sedimentation, sand filtration postozonation, and biological activated carbon (BAC) filtration. The results indicated that heterotrophic plate counts decreased dramatically through the drinking water treatment processes. Clone library analysis indicated the significant change of bacterial community structure through the water treatment processes. Betaproteobacteria was dominant in raw water, the sand filter effluent and the BAC filter effluent. This work could provide some new insights on drinking water microbial ecology.

Keywords

Biological activated carbon Drinking water treatment system Sand filter Microbial community 

Notes

Acknowledgments

This work was financially supported by the Major Science and Technology Program for Water Pollution Control and Treatment in China (No. 2009ZX07423-003).

References

  1. APHA, Awwa, WEF (1995) Standard methods for the examination of water and wastewater, 19th edn. American Public Health Association, Washington, DCGoogle Scholar
  2. Berry D, Xi CW, Raskin L (2006) Microbial ecology of drinking water distribution systems. Curr Opin Biotechnol 17(3):297–302CrossRefGoogle Scholar
  3. Burlingame GA, Suffet IH, Pipes WO (1986) Predominant bacterial genera in granular activated carbon water treatment systems. Can J Microbiol 32(3):226–230CrossRefGoogle Scholar
  4. China Environmental Protection Agency (2002) Methods for water and wastewater determination. China Environmental Science Press, BeijingGoogle Scholar
  5. Domingo JWS, Meckes MC, Simpson JM, Sloss B, Reasoner DJ (2003) Molecular characterization of bacteria inhabiting a water distribution system simulator. Water Sci Technol 47(5):149–154Google Scholar
  6. Eichler S, Christen R, Holtje C, Westphal P, Botel J, Brettar I, Mehling A, Hofle MG (2006) Composition and dynamics of bacterial communities of a drinking water supply system as assessed by RNA- and DNA-based 16S rRNA gene fingerprinting. Appl Environ Microbiol 72(3):1858–1872CrossRefGoogle Scholar
  7. Feng S, Zhang XJ, Wang QF, Wan R, Chen C, Xie SG (2012) Heterogeneity of ammonia-oxidizing community structures in a pilot-scale drinking water biofilter. Int Biodeterior Biodegrad 70:148–152CrossRefGoogle Scholar
  8. Feng S, Chen C, Wang QF, Zhang XJ, Yang ZY, Xie SG (2013a) Characterization of microbial communities in a granular activated carbon-sand dual media filter for drinking water treatment. Int J Environ Sci Technol 10(5):917–922CrossRefGoogle Scholar
  9. Feng S, Chen C, Wang QF, Yang ZY, Zhang XJ, Xie SG (2013b) Microbial community in a full-scale drinking water biosand filter. J Environ Biol 34:321–324Google Scholar
  10. Huang Y, Zou L, Zhang SY, Xie SG (2011) Comparison of bacterioplankton communities in three heavily polluted streams in China. Biomed Environ Sci 24(2):140–145Google Scholar
  11. Kasuga I, Shimazaki D, Kunikane S (2007) Influence of backwashing on the microbial community in a biofilm developed on biological activated carbon used in a drinking water treatment plant. Water Sci Technol 55(8–9):173–180CrossRefGoogle Scholar
  12. Kormas KA, Neofitou C, Pachiadaki M, Koufostathi E (2010) Changes of the bacterial assemblages throughout an urban drinking water distribution system. Environ Monit Assess 165(1–4):27–38CrossRefGoogle Scholar
  13. Li X, Upadhyaya G, Yuen W, Brown J, Morgenroth E, Raskin L (2010) Changes in the structure and function of microbial communities in drinking water treatment bioreactors upon addition of phosphorus. Appl Environ Microbiol 76(22):7473–7481CrossRefGoogle Scholar
  14. Liao XB, Chen C, Chang CH, Wang Z, Zhang XJ, Xie SG (2012) Heterogeneity of microbial community structures inside the up-flow biological activated carbon (BAC) filters for the treatment of drinking water. Biotechnol Bioprocess Eng 17(4):881–886CrossRefGoogle Scholar
  15. Liao XB, Chen C, Wang Z, Wan R, Chang CH, Zhang XJ, Xie SG (2013) Changes of biomass and bacterial communities in biological activated carbon filters for drinking water treatment. Process Biochem 48(2):312–316CrossRefGoogle Scholar
  16. Lu PP, Chen C, Wang QF, Wang Z, Zhang XJ, Xie SG (2013) Phylogenetic diversity of microbial communities in real drinking water distribution systems. Biotechnol Bioprocess Eng 18(1):119–124CrossRefGoogle Scholar
  17. Niemi RM, Heiskanen I, Heine R, Rapala J (2009) Previously uncultured β-Proteobacteria dominate in biologically active granular activated carbon (BAC) filters. Water Res 43(20):5075–5086CrossRefGoogle Scholar
  18. Norton CD, LeChevallier MW (2000) A pilot study of bacteriological population changes through potable water treatment and distribution. Appl Environ Microbiol 66(1):268–276CrossRefGoogle Scholar
  19. Pavlov D, de Wet CME, Grabow WOK, Ehlers MM (2004) Potentially pathogenic features of heterotrophic plate count bacteria isolated from treated and untreated drinking water. Int J Food Microbiol 92(3):275–287CrossRefGoogle Scholar
  20. Pinto AJ, Xi CW, Raskin L (2012) Bacterial community structure in the drinking water microbiome is governed by filtration processes. Environ Sci Technol 46(16):8851–8859CrossRefGoogle Scholar
  21. Poitelon JB, Joyeux M, Welte B, Duguet JP, Prestel E, DuBow MS (2010) Variations of bacterial 16S rDNA phylotypes prior to and after chlorination for drinking water production from two surface water treatment plants. J Ind Microbiol Biotechnol 37(2):117–128CrossRefGoogle Scholar
  22. Revetta RP, Pemberton A, Lamendella R, Iker B, Domingo JWS (2010) Identification of bacterial populations in drinking water using 16S rRNA-based sequence analyses. Water Res 44(5):1353–1360CrossRefGoogle Scholar
  23. Sartory DP, Gu HY, Chen CM (2008) Comparison of a novel MPN method against the yeast extract agar (YEA) pour plate method for the enumeration of heterotrophic bacteria from drinking water. Water Res 42(13):3489–3497CrossRefGoogle Scholar
  24. Schloss PD, Handelsman J (2005) Introducing DOTUR, a computer program for defining operational taxonomic units and estimating species richness. Appl Environ Microbiol 71(3):1501–1506CrossRefGoogle Scholar
  25. Soonglerdsongpha S, Kasuga I, Kurisu F, Furumai H (2011) Comparison of assimilable organic carbon removal and bacterial community structures in biological activated carbon process for advanced drinking water treatment plants. Sustain Environ Res 21(1):59–64Google Scholar
  26. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4, molecular evolutionary genetics analysis, MEGA software version 4.0. Mol Biol Evol 24(8):1596–1599CrossRefGoogle Scholar
  27. Villarreal JV, Schwartz T, Obst U (2010) Culture-independent techniques applied to food industry water surveillance: a case study. Int J Food Microbiol 141(SI 1):S147–S155CrossRefGoogle Scholar
  28. Wang Q, Garrity GM, Tiedje JM, Cole JR (2007) Naïve Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol 73(16):5261–5267CrossRefGoogle Scholar
  29. Wang Y, Wan R, Zhang SY, Xie SG (2012) Anthracene biodegradation under nitrate-reducing condition and associated microbial community changes. Biotechnol Bioprocess Eng 17(2):371–376CrossRefGoogle Scholar
  30. Williams MM, Domingo JWS, Meckes MC, Kelty CA, Rochon HS (2004) Phylogenetic diversity of drinking water bacteria in a distribution system simulator. J Appl Microbiol 96(5):954–964CrossRefGoogle Scholar
  31. Yapsakli K, Cecen F (2010) Effect of type of granular activated carbon on DOC biodegradation in biological activated carbon filters. Process Biochem 45(3):355–362CrossRefGoogle Scholar
  32. Zhang SY, Wang QF, Xie SG (2012) Molecular characterization of phenanthrene-degrading methanogenic communities in leachate-contaminated aquifer sediment. Int J Environ Sci Technol 9(4):705–712CrossRefGoogle Scholar
  33. Zwart G, Crump BC, Agterveld MK, Hagen F, Han SK (2002) Typical freshwater bacteria: an analysis of available 16S rRNA gene sequences from plankton of lakes and rivers. Aquat Microb Ecol 28(2):141–155CrossRefGoogle Scholar

Copyright information

© Islamic Azad University (IAU) 2014

Authors and Affiliations

  • X. Liao
    • 1
  • C. Chen
    • 1
  • Z. Wang
    • 2
  • C.-H. Chang
    • 2
  • X. Zhang
    • 1
  • S. Xie
    • 2
  1. 1.School of EnvironmentTsinghua UniversityBeijingChina
  2. 2.College of Environmental Sciences and Engineering, The Key Laboratory of Water and Sediment Sciences (Ministry of Education)Peking UniversityBeijingChina

Personalised recommendations