Phytoremediation of soil contaminated by heavy oil with plants colonized by mycorrhizal fungi

  • H.-C. Kuo
  • D.-F. Juang
  • L. Yang
  • W.-C. Kuo
  • Y.-M. Wu
Original Paper


The purpose of this study was to investigate the effect of phytoremediation on soils contaminated with heavy crude oil using plants infected by mycorrhizal fungi. Five plant species, Vetiveria zizanioides, Bidens pilosa, Chloris barbata, Eleusine indica, and Imperata cylindrica, infected with the species of mycorrhizal fungi Glomus mosseae, were selected for this study. The degradation of total petroleum hydrocarbons in soils and several physiological parameters of plants such as shoot length and biomass were analyzed. Out of the 5 plant species tested, only V. zizanioides, B. pilosa, and E. indica could take up the G. mosseae. Out of these three, V. zizanioides showed the greatest growth (biomass) in soils with 100,000 mg kg−1 total petroleum hydrocarbons. In addition, B. pilosa infected with G. mosseae was found to be able to increase degradation by 9 % under an initial total petroleum hydrocarbons concentration of 30,000 mg kg−1 in soils after 64 days. We conclude that plants infected with mycorrhizal fungi can enhance the phytoremediation efficiency of soils contaminated with high concentrations of heavy oil.


Crude oil Mycorrhiza Rhizoremediation Total petroleum hydrocarbons 



The project was conducted at Department of Marine Environment and Engineering National Sun Yat-sen University. The authors are grateful for the use of facilities, and the authors would like to thank ROC National Science Council (NSC96-2221-E-110-023-MY3) for providing funding for the research.


  1. Agamuthu P, Abioye OP, Aziz AA (2010) Phytoremediation of soil contaminated with used lubricating oil using Jatropha curcas. J Hazard Mater 179(1–3):891–894CrossRefGoogle Scholar
  2. Alkorta I, Garbisu C (2001) Phytoremediation of organic contaminants in soils. Bioresour Technol 79:273CrossRefGoogle Scholar
  3. Barrett G, Campbell CD, Fitter AH, Hodge A (2011) The arbuscular mycorrhizal fungus Glomus hoi can capture and transfer nitrogen from organic patches to its associated host plant at low temperature. Appl Soil Ecol 48(1):102–105CrossRefGoogle Scholar
  4. Bragg JR, Prince RC, Harner EJ, Atlas RM (1994) Effectiveness of bioremediation for the Exxon Valdez oil spill. Nature 368:413CrossRefGoogle Scholar
  5. Burken JG, Schnoor JL (1996) Phytoremediation: plant uptake of atrazine and role of exudates. J Environ Eng 1222:958CrossRefGoogle Scholar
  6. Ferro AM, Sims RC, Bugbee B (1994) Hycrest crested wheatgrass accelerates the degradation of pentachlorophenol in soil. J Environ Qual 23:272CrossRefGoogle Scholar
  7. Fingas M (2001) The basics of oil spill cleanup. Lewis Publishers, New YorkGoogle Scholar
  8. Gao Y, Zhu L (2004) Plant uptake, accumulation and translocation of phenanthrene and pyrene in soil. Chemosphere 55:1169CrossRefGoogle Scholar
  9. Gao Y, Li Q, Ling W, Zhu X (2011) Arbuscular mycorrhizal phytoremediation of soils contaminated with phenanthrene and pyrene. J Hazard Mater 185(2–3):703–709CrossRefGoogle Scholar
  10. Gerhardt KE, Huang XD, Glick BR, Greenberg BM (2009) Phytoremediation and rhizoremediation of organic soil contaminants: potential and challenges. Plant Sci 176:20CrossRefGoogle Scholar
  11. Glick BR (2003) Phytoremediation: synergistic use of plants and bacteria to clean up the environment. Biotechnol Adv 21:383CrossRefGoogle Scholar
  12. Hamdi H, Benzarti S, Aoyama I, Jedidi N (2012) Rehabilitation of degraded soils containing aged PAHs based on phytoremediation with alfalfa (Medicago sativa L.). Int Biodeterior Biodegrad 67:40–47CrossRefGoogle Scholar
  13. Hodson PV, Ibrahim I, Zambon S, Ewert A, Lee K (2002) Bioavailability to fish of sediment PAHs as an indicator of the success of onsite remediation treatment at an experiment oil spill. Bioremediat J 6:297CrossRefGoogle Scholar
  14. Huang XD, El-Alawi Y, Penrose DM, Glick BR, Greenberg BM (2004) A multi-process phytoremediation system for removal of polycyclic hydrocarbons form contaminated soils. Environ Pollut 130:465CrossRefGoogle Scholar
  15. Joner EJ, Leyval C, Colpaert JV (2006) Ectomycorrhizas impede phytoremediation of polycyclic aromatic hydrocarbons (PAHs) both within and beyond the rhizosphere. Environ Pollut 142:34CrossRefGoogle Scholar
  16. Ke L, Wang WQ, Wong TWY, Wong YS, Tam NFY (2003) Removal of pyrene from contaminated sediments by mangrove microcosms. Chemosphere 51:25CrossRefGoogle Scholar
  17. Lin Q, Mendelssohn IA (1998) The combined effects of phytoremediation and biostimulation in enhancing habitat restoration and oil degradation of petroleum contaminated wetlands. Ecol Eng 10:263CrossRefGoogle Scholar
  18. Liste HH, Prutz I (2006) Plant performance, dioxygenaseexpressing rhizosphere bacteria and bioremediation of weathered hydrocarbons in contaminated soil. Chemosphere 62:1411CrossRefGoogle Scholar
  19. Marchiol L, Assolari S, Sacco P, Zerb G (2004) Phytoremediation of heavy metals by canola (Brassica napus) and radish (Raphanus sativus) grown on multicontaminated soil. Environ Pollut 132:21CrossRefGoogle Scholar
  20. Melo MR, Flores NR, Murrieta SV, Tovar AR, Zúñiga AG, Hernández OF, Mendoza AP, Pérez NO, Dorantes AR (2011) Comparative plant growth promoting traits and distribution of rhizobacteria associated with heavy metals in contaminated soils. Int J Environ Sci Tech 8(4):807–816Google Scholar
  21. Moreira ITA, Oliveira OMC, Triguis JA, Santos AMP, Queiroz AFS, Martins CMS, Silva CS, Jesus RS (2011) Phytoremediation using Rizophora mangle L. in mangrove sediments contaminated by persistent total petroleum hydrocarbons (TPH’s). Microchem J 99(2):376–382CrossRefGoogle Scholar
  22. Nicolotti G, Egli S (1998) Soil contamination by crude oil: impact on the mycorrhizosphere acid on the revegetation potential of forest trees. Environ Pollut 99:37CrossRefGoogle Scholar
  23. Orłowska E, Godzik B, Turnau K (2012) Effect of different arbuscular mycorrhizal fungal isolates on growth and arsenic accumulation in Plantago lanceolata L. Environ Pollut 168:121–130CrossRefGoogle Scholar
  24. Parrish ZD, Banks MK, Schwab AP (2005) Assessment of contaminant lability during phytoremediation of polycyclic aromatic hydrocarbon impacted soil. Environ Pollut 137:187CrossRefGoogle Scholar
  25. Pichtel J, Liskanen P (2001) Degradation of diesel fuel in rhizosphere soil. Environ Eng Sci 18:145CrossRefGoogle Scholar
  26. Pradhan SP, Conrad JR, Paterek JR, Srivastava VJ (1998) Potential of phytoremediation for treat of PAHs in soil at MGP sites. J Soil Contam 7:467CrossRefGoogle Scholar
  27. Rahman KSM, Rahman TJ, Kourkoutas Y, Petsas I, Marchant R, Banat IM (2003) Enhanced bioremediation of n-alkane in petroleum sludge using bacteria consortium amended with rhamnolipid and micronutrients. Bioresour Technol 90:159CrossRefGoogle Scholar
  28. Sung K, Munster CL, Rhykerd R, Drew MC (2003) The use of vegetation to remediate soil freshly contaminated by recalcitrant contaminants. Water Res 37:2408CrossRefGoogle Scholar
  29. Taheri WI, Bever JD (2011) Adaptation of Liquidambar styraciflua to coal tailings is mediated by arbuscular mycorrhizal fungi. Appl Soil Ecol 48(2):251–255CrossRefGoogle Scholar
  30. Wang JY, Yang L, Tseng CC, Hsu HL (2008) Application of phytoremediation on soil contaminated by pyrene. Environ Eng Sci 25:6Google Scholar
  31. Wang MC, Chen YT, Chen SH, Chang Chien SW, Sunkara SV (2012) Phytoremediation of pyrene contaminated soils amended with compost and planted with ryegrass and alfalfa. Chemosphere 87(3):217–225CrossRefGoogle Scholar
  32. Willumsen PA, Arvin E (1999) Kinetic of degradation of surfactant-solubilized fluoranthene by a Sphingomonas paucimobilis. Environ Sci Technol 33:2571CrossRefGoogle Scholar
  33. Yang, L., Kao, J.M., Liou, J.K. (2007). Research of potassium permanganate oxidation method and phytoremediation of soil contaminated by heavy oil. CPC corporation Taiwan project report, Project No. eea9413001Google Scholar
  34. Yoshitomi KJ, Shann JR (2001) Corn (Zea mays L.) root exudates and their impact on 14C-pyrene mineralization. Soil Biol Biochem 33:1769CrossRefGoogle Scholar
  35. Zhang Z, Rengel Z, Chang H, Meney K, Pantelic L, Tomanovic R (2012) Phytoremediation potential of Juncus subsecundus in soils contaminated with cadmium and polynuclear aromatic hydrocarbons (PAHs). Geoderma 175–176:1–8CrossRefGoogle Scholar

Copyright information

© Islamic Azad University (IAU) 2013

Authors and Affiliations

  • H.-C. Kuo
    • 1
  • D.-F. Juang
    • 3
  • L. Yang
    • 1
  • W.-C. Kuo
    • 2
  • Y.-M. Wu
    • 1
  1. 1.Department of Marine Environment and EngineeringNational Sun Yat-sen UniversityKaohsiung 804Taiwan, ROC
  2. 2.Department of Environmental Science and EngineeringNational Pingtung University of Science and TechnologyNeipu, Pingtung 912Taiwan, ROC
  3. 3.Department of Health Business AdministrationMeiho UniversityNeipu, Pingtung 912Taiwan, ROC

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