Interactions between algicidal bacteria and the cyanobacterium Microcystis aeruginosa: lytic characteristics and physiological responses in the cyanobacteria
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Application of algicidal bacteria is a promising and environmentally friendly way to control cyanobacterial blooms. Lytic effects of the algicidal bacteria on Microcystis aeruginosa have been observed, but the interactions between algicidal bacteria and the cyanobacteria are still elusive. An algicidal bacterium Bacillus sp. B50 isolated from Lake Donghu showed a highly lytic efficiency on M. aeruginosa NIES-843 through heat-resistant extracellular substances from strain B50. The cell density of strain B50 could be maintained at high levels during the lytic process in bacteria–Microcystis system with inoculation densities of 1.9 × 106 and 1.9 × 107 cfu/mL, resulting in the death of M. aeruginosa NIES-843. However, the population dynamics of strain B50 was a bell-shaped curve at low inoculation densities and no lytic effect could be observed. Results of physiological responses suggested that the lytic efficiency may be mediated through inhibition of metabolism and production of reactive oxygen species.
KeywordsBacillus sp. Gene expressions Lytic characteristics Microcystis aeruginosa Oxidative stress Photosynthesis inhibition
The research was supported by National Natural Science Foundation of China (No. 21107024), Hunan Provincial Natural Science Foundation of China (10JJ6045), and the Foundation of Furong Scholar project of Hunan Province. Professor Joe Lepo (University of West Florida) provided language assistance and useful suggestions in an early draft of this manuscript.
- Carmichael WW (1995) Toxic Microcystis in the environment. In: Watanabe MF, Harada K, Carmichael WW, Fujiki H (eds) Toxic Microcystis. CRC Press, New York, pp 1–12Google Scholar
- Griffiths HR (2005) Chemical modifications of biomolecules by oxidants. Handb Environ Chem 2:33–62Google Scholar
- Ichimura T (1979) Media for freshwater cyanobacteria. In: Nishizawa K, Chihara M (eds) Methods in phycology. Kyouritsu Shuppan, Tokyo, pp 295–296Google Scholar
- Nübel U, Garcia-Pichel F, Muyzer G (1997) PCR primers to amplify 16S rRNA genes from cyanobacteria. Appl Environ Microbiol 63:3327–3332Google Scholar
- Richards FA, Thompson TG (1952) The estimation and characterization of plankton populations by pigment analyses. II. A spectrophotometric method for the estimation of plankton pigments. J Marine Res 11:156–172Google Scholar
- Yang CY, Zhou SW, Xia CH, Liu YD (2009) The effect of microcystin-RR on the growth and physiological characteristics of denitrifying bacteria. Chin J Environ Pollut Control 31:7–10Google Scholar