, Volume 11, Issue 4, pp 137–144 | Cite as

Antioxidant activities of various solvent extracts of custard apple (Annona squamosa L.) fruit pulp

  • Umesh Balkrishna Jagtap
  • Vishwas Anant BapatEmail author
Original Research


The study aimed to investigate the effect of different solvent (water, methanol, ethanol and acetone) extractions on total phenolic content (TPC) and antioxidant properties of custard apple (Annona squamosa L.) fruit pulp (CAFP) extracts. Various antioxidant assays including 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, ferric-reducing antioxidant power (FRAP) and N,N-dimethyl-pphenylendiamine (DMPD) radical scavenging assay were used for evaluation of antioxidant activity of extracts in vitro. CAFP extracted with acetone showed the highest amount of TPC (1.43±0.65 mg GAE g−1 FW) and antioxidant capacity determined by DPPH (96.11±0.09%) and FRAP (3.475±0.002 O.D.) assay whereas water extract had the highest DMPD scavenging capacity (37.62±0.24%). The presence of catechol and vanillin was detected using RP-HPLC. In this sense, the nature of extracting solvents significantly influenced the TPC as well as antioxidant properties of the CAFP.


Annona squamosa L. antioxidant capacity DPPH RP-HPLC total phenolics 


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Copyright information

© CEC editore - Springer-Verlag Italia 2012

Authors and Affiliations

  • Umesh Balkrishna Jagtap
    • 1
  • Vishwas Anant Bapat
    • 1
    Email author
  1. 1.Department of BiotechnologyShivaji UniversityVidyanagarIndia

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