Neotropical Entomology

, Volume 46, Issue 3, pp 256–263 | Cite as

Gamma irradiation on canola seeds affects herbivore-plant and host-parasitoid interactions

  • M Akandeh
  • M SoufbafEmail author
  • F Kocheili
  • A Rasekh
Ecology, Behavior and Bionomics


As an agricultural modernization, gamma irradiation is an important method for enhancing crop yield and quality. Nevertheless, its use can alter other plant traits such as nutrition and resistance to different biotic/abiotic stresses that consequently affect plant–insect interactions. A tritrophic system was utilized based on two canola mutant lines produced through gamma irradiation (RGS 8–1 and Talaye 8–3). Plutella xylostella (L.), as a worldwide pest of Brassicaceae and Cotesia vestalis (Holiday) as a key biocontrol agent of P. xylostella were examined for the potential indirect effects of canola seed irradiation on the experimental insects’ performance when acting on the respective mutant lines. This study showed that physical mutation did not affect plant nitrogen and herbivore-damaged total phenolics; however, phenolic compounds showed greater concentration in damaged leaves than undamaged leaves of both mutant and control plants. The relative growth rate and pupal weight of P. xylostella reared on RGS 8–1 were significantly higher than those reared on the control RGS. There was no significant difference by performance parameters of the parasitoid, C. vestalis, including total pre-oviposition period, adult longevity, adult fresh body weight of males and females, pupal weight, forewing area, and total longevity of both sexes on tested canola cultivars in comparison with their mutant lines. Life table parameters of C. vestalis on mutant lines of both cultivars, RGS and Talaye, were not significantly different from their control treatments. Comprehensive studies should be conducted to find out the mechanisms under which gamma rays affect plant–insect interactions.


Diamondback moth Insect fitness Host plant resistance Physical mutation Total phenolics Tritrophic interactions 



This research is a part of Ph.D. dissertation of M.A. that was funded by Shahid Chamran University. We wish to thank Kamran Mozafari for providing us with mutant canola genotypes.


  1. Ahloowalia B, Maluszynski M (2001) Induced mutations–a new paradigm in plant breeding. Euphytica 118:167–173CrossRefGoogle Scholar
  2. Akandeh M, Kocheili F, Soufbaf M, Rasekh A (2015) The effect of physical mutation on the life history parameters and ovipositional preference of the Plutella xylostella. J Crop Prot 4:633–642Google Scholar
  3. Akandeh M, Kocheili F, Soufbaf M, Rasekh A, Mozafari K (2016) Effect of canola’ physical mutation on plutella xylostella (L.) life table. J Agric Sci Technol 18(4): 985–998Google Scholar
  4. Aqueel MA, Leather SR (2011) Effect of nitrogen fertilizer on the growth and survival of Rhopalosiphum padi (L.) and Sitobion avenae (F.) (Homoptera: Aphididae) on different wheat cultivars. Crop Prot 30:216–221CrossRefGoogle Scholar
  5. Badenes-Perez FR, Reichelt M, Heckel DG (2010) Can sulfur fertilisation improve the effectiveness of trap crops for diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)? Pest Manag Sci 66:832–838PubMedGoogle Scholar
  6. Bryman A, Cramer D (2002) Quantitative data analysis with SPSS release 10 for Windows (A guide for social scientists), LondonGoogle Scholar
  7. Campbell BC, Duffey SS (1979) Tomatine and parasitic wasps: potential incompatibility of plant antibiosis with biological control. Science 205:700–702CrossRefPubMedGoogle Scholar
  8. Chi H (1988) Life-table analysis incorporating both sexes and variable development rate among individuals. Environ Entomol 17:26–34CrossRefGoogle Scholar
  9. Chi H (2015) TWOSEX-MS Chart: a computer program for the age-stage, two-sex life table analysis. URL:
  10. Ciss M, Parisey N, Fournier G, Taupin P, Dedryver C, Pierre J (2014) Response of insect relative growth rate to temperature and host-plant phenology: estimation and validation from field data. PLoS ONE 9(1):86825. doi: 10.1371/journal.pone.0086825 CrossRefGoogle Scholar
  11. Coley PD, Bateman M, Kursar T (2006) The effects of plant quality on caterpillar growth and defense against natural enemies. Oikos 115:219–228CrossRefGoogle Scholar
  12. De Kraker J, Rabbinge R, Van Huis A, Van Lenteren JC, Heong KL (2000) Impact of nitrogenous fertilization on the population dynamics and natural control of rice leaffolders (Lep.: Pyralidae). Int J Pest Manag 46:225–235CrossRefGoogle Scholar
  13. Dhanavel D, Gnanamurthy S, Girija M (2012) Effect of gamma rays on induced chromosomal variation in cowpea Vigna unguiculata (L.) Walp. Int J Curr Sci 2012:245–250Google Scholar
  14. Faeth SH (1990) Structural damage to oak leaves alters enemy attack on a leaf-miner. Entomol Exp Appl 57:57–63CrossRefGoogle Scholar
  15. Fisher AEI, Hartley SE, Young M (2000) Direct and indirect competitive effects of foliage feeding guilds on the performance of the birch leaf-miner Eriocrania. J Anim Ecol 69:165–176CrossRefGoogle Scholar
  16. Fox LR, Letourneau DK, Eisenbach J, Nouhuys SV (1990) Parasitism rates and sex ratios of a parasitoid wasp: effects of herbivore and plant quality. Oecologia 83:414–419CrossRefPubMedGoogle Scholar
  17. Gange AC, Brown VK (1989) Effects of root herbivory by an insect on a foliar-feeding species, mediated through changes in the host plant. Oecologia 81:38–42CrossRefPubMedGoogle Scholar
  18. Godfray HCJ, Crute IR, Haddad L, Lawrence D, Muir JF, Nisbett N, Pretty J, Robinson S, Toulmin C, Whiteley R (2010) The future of the global food system. Phil Trans Royal Soc London B: Biol Sci 365:2769–2777CrossRefGoogle Scholar
  19. Gols R, Witjes LMA, Van Loon JJA, Posthumus MA, Dicke M, Harvey JA (2008) The effect of direct and indirect defenses in two wild brassicaceous plant species on a specialist herbivore and its gregarious endoparasitoid. Entomol Exp Appl 128:99–108CrossRefGoogle Scholar
  20. Hails RS (2002) Assessing the risks associated with new agricultural practices. Nature 418:685–688CrossRefPubMedGoogle Scholar
  21. Hanhimaki S, Senn J, Haukioja E (1994) Performance of insect herbivores on hybridising trees: the case of the sub-arctic birches. J Anim Ecol 63:163–175CrossRefGoogle Scholar
  22. Hunter MD (2003) Effects of plant quality on the population ecology of parasitoids. Agric For Entomol 5:1–8CrossRefGoogle Scholar
  23. Jahn GC, Almazan LP, Pacia JB (2005) Effect of nitrogen fertilizer on the intrinsic rate of increase of Hysteroneura setariae (Thomas) (Homoptera: Aphididae) on Rice (Oryza sativa L.). Environ Entomol 34:938–943CrossRefGoogle Scholar
  24. Kant R, Minor MA, Trewick SA (2012) Fitness gain in a koinobiont parasitoid Diaeretiella rapae (Hymenoptera: Braconidae) by parasitising hosts of different ages. J Asia Pac Entomol 15:83–87CrossRefGoogle Scholar
  25. Karla YP (1998) Reference methods for plant analysis. Soil and Plant Analysis Council, Inc, Boca RatonGoogle Scholar
  26. Leather SR, Dixon AFG (1984) Aphid growth and reproductive rates. Entomol Exp Appl 35:137–140CrossRefGoogle Scholar
  27. Mackauer M, Sequeira R (1993) Patterns of development in insect parasites. Parasites and pathogens of insects. (eds. Beckage NE, Thompson SN, Federici BA), pp 1–23. New YorkGoogle Scholar
  28. Makkar HPS, Bluemmel M, Borowy NK, Becker K (1993) Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods. J Sci Food Agric 61:161–165CrossRefGoogle Scholar
  29. Marazzi C, Stadler E (2004) Influence of plant sulphur nutrition on oviposition and larval performance of the diamondback moth. Entomol Exp Appl 111:225–232CrossRefGoogle Scholar
  30. Minkenberg OPJM, Ottenheim JJGW (1990) Effect of leaf nitrogen content of tomato plants on preference and performance of a leafmining fly. Oecologia 83:291–298CrossRefPubMedGoogle Scholar
  31. Nikooei M, Fathipour Y, Jalali- Javaran M, Soufbaf M (2015) How different genetically manipulated brassica genotypes affect life table parameters of Plutella xylostella (Lepidoptera: Plutellidae). J Econ Entomol 108:515–524CrossRefPubMedGoogle Scholar
  32. Ode PJ (2006) Plant chemistry and natural enemy fitness: effects on herbivore and natural enemy interactions. Annu Rev Entomol 51:163–185CrossRefPubMedGoogle Scholar
  33. Petermann JS, Müller CB, Roscher C, Weigelt A, Weisser WW, Schmid B (2010) Plant species loss affects life-history traits of aphids and their parasitoids. PLoS One 5:e12053. doi: 10.1371/journal.pone.0012053 CrossRefPubMedPubMedCentralGoogle Scholar
  34. Prudic K, Oliver J, Bowers M (2005) Soil nutrient effects on oviposition preference, larval performance, and chemical defense of a specialist insect herbivore. Oecologia 143:578–587CrossRefPubMedGoogle Scholar
  35. Romeis J, Meissle M, Bigler F (2006) Transgenic crops expressing Bacillus thuringiensis toxins and biological control. Nat Biotechnol 24:63–71CrossRefPubMedGoogle Scholar
  36. Sarfraz RM, Dosdall LM, Blake AJ, Keddie BA (2010) Leaf nutrient levels and the spatio-temporal distributions of Plutella xylostella and its larval parasitoids Diadegma insulare and Microplitis plutellae in canola. BioControl 55:229–244CrossRefGoogle Scholar
  37. Singer MS, Timothy EF, Christian MS, Kailen AM (2012) Tritrophic interactions at a community level: effects of host plant species quality on bird predation of caterpillars. Am Nat 179:000. doi: 10.5061/dryad.15535rh1 CrossRefGoogle Scholar
  38. Soufbaf M, Fathipour Y, Zalucki MP, Hui C (2012) Importance of primary metabolites in canola in mediating interactions between a specialist leaf-feeding insect and its specialist solitary endoparasitoid. Arthropod Plant Interact 6:241–250CrossRefGoogle Scholar
  39. SPSS 2008 SPSS base 16.0.2 for Windows user’s guide. SPSS IncGoogle Scholar
  40. Teder T, Tammaru T (2002) Cascading effects of variation in plant vigour on the relative performance of insect herbivores and their parasitoids. Ecol Entomol 27:94–104CrossRefGoogle Scholar
  41. Van Loon JJA, Wang CZ, Nielsen JK, Gols R, Qiu YT (2002) Flavonoids from cabbage are feeding stimulants for diamondback moth larvae additional to glucosinolates chemoreception and behaviour. Entomol Exp Appl 104:27–34CrossRefGoogle Scholar

Copyright information

© Sociedade Entomológica do Brasil 2016

Authors and Affiliations

  1. 1.Dept of Entomology, Faculty of AgricultureShahid Chamran Univ of AhvazAhvazIran
  2. 2.Agricultural, Medical and Industrial Research SchoolKarajIran

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