Journal of the Iranian Chemical Society

, Volume 16, Issue 3, pp 609–615 | Cite as

A novel method for the simultaneous determination of 5-fluorouracil and oxaliplatin in new biodegradable PHBV/PLGA nanoparticles

  • Somayeh Handali
  • Zahra Ramezani
  • Eskandar Moghimipour
  • Mohsen Rezaei
  • Farid Abedin DorkooshEmail author
Original Paper


The aim of the present study was to develop a novel HPLC method for the simultaneous determination of 5-fluorouracil (5-FU) and oxaliplatin (OXA) in the PHBV/PLGA nanoparticles (NPs). Chromatographic separation was performed using C18 column at a detection wavelength of 260 nm. The mobile phase consisted of 0.02 M phosphate buffer (pH 4) and methanol (90:10, V/V) and flow rate was 0.8 mL min−1. Column temperature was also maintained at 30 °C. The calibration curves displayed linear concentration ranges of 0.05–40 µg mL−1 for 5-FU and 0.5–80 µg mL−1 for OXA with correlation coefficients of 0.998. The LOD and LOQ were, respectively, 0.01 and 0.03 µg mL−1 for 5-FU and 0.13 and 0.44 µg mL−1 for OXA. The values of intra- and inter-day precision and accuracy were within acceptable limits. According to the results, the method was found to be simple, reliable, rapid, and reproducible. In addition, the HPLC assay method presented was successfully applied to the simultaneous determination of 5-FU and OXA in PHBV/PLGA NPs.


5-Fluorouracil Oxaliplatin HPLC PHBV PLGA Nanoparticles 



The work was financially supported by Nanotechnology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran (grant No. 111).

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflict of interest.


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Copyright information

© Iranian Chemical Society 2018

Authors and Affiliations

  • Somayeh Handali
    • 1
  • Zahra Ramezani
    • 1
  • Eskandar Moghimipour
    • 1
    • 2
  • Mohsen Rezaei
    • 3
  • Farid Abedin Dorkoosh
    • 4
    • 5
    Email author
  1. 1.Nanotechnology Research CenterAhvaz Jundishapur University of Medical SciencesAhvazIran
  2. 2.Cellular and Molecular Research CenterAhvaz Jundishapur University of Medical SciencesAhvazIran
  3. 3.Department of Toxicology, Faculty of Medical SciencesTarbiat Modares UniversityTehranIran
  4. 4.Department of Pharmaceutics, Faculty of PharmacyTehran University of Medical SciencesTehranIran
  5. 5.Medical Biomaterial Research Centre (MBRC)Tehran University of Medical SciencesTehranIran

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