Journal of the Iranian Chemical Society

, Volume 15, Issue 11, pp 2585–2592 | Cite as

Evaluation of polypyrrole/silver/polyethylene glycol nanocomposite sorbent for electroenhanced direct-immersion solid-phase microextraction of carvacrol and thymol from medicinal plants

  • Ali Reza Ghiasvand
  • Sana Abdolhosseini
  • Nahid Heidari
  • Brett Paull
Original Paper


The unique features of nanostructured polypyrrole, conductivity enhancement effect of silver nanoparticles and high polar adsorptivity of polyethylene glycol were merged into polypyrrole/silver/polyethylene glycol (PPy/Ag/PEG) nanocomposite. It was synthesized and simultaneously coated on the surface of a stainless-steel fiber using an amended electropolymerization procedure. Before coating, the fiber substrate was made porous and sticky by allocating platinum dots on the surface of the stainless-steel fiber using the electrophoretic method. The prepared fiber was applied for the extraction of carvacrol and thymol (the most important antioxidants in medicinal plants) through an electroenhanced direct-immersion solid-phase microextraction (EE-DI-SPME) sampling strategy, followed by GC-FID quantification. To achieve the best efficiency, the effectual experimental variables including pH of sample solution, applied voltage, extraction temperature and time, stirring rate, and ionic strength were investigated. Under the optimal experimental conditions, the calibration curves were linear over the range of 0.5–30 µg mL−1 for thymol and 0.01–30 µg mL−1 for carvacrol. The detection limits (3Sb) and relative standard deviation (RSD%, n = 6) were obtained to be 0.15, 0.003 µg mL−1 and 10.2, 8.7% for thymol and carvacrol, respectively. The results demonstrated the priority of the proposed fiber compared with polypyrrole and polyacrylate fibers, in terms of extraction efficiency, durability and stability. The developed method was successfully employed for the analysis of thymol and carvacrol in medicinal plants.


Electroenhanced direct-immersion solid-phase microextraction Polypyrrole/silver/polyethylene glycol nanocomposite Antioxidants GC-FID 


  1. 1.
    G.M.L. Fiori, P.S. Bonato, M.P.M. Pereira, S.H.T. Contini, A.M.S. Pereira, J. Braz. Chem. Soc. 24, 837–846 (2013)Google Scholar
  2. 2.
    J.B. Engel, C. Heckler, E.C. Tondo, D.J. Daroit, P.S. Malheiros, Int. J. Food Microbiol. 252, 18–23 (2017)CrossRefGoogle Scholar
  3. 3.
    M. Roosta, M. Ghaedi, A. Daneshfar, R. Sahraei, J. Chromatogr. B 975, 34–39 (2015)CrossRefGoogle Scholar
  4. 4.
    M. Asadollahi-Baboli, A. Aghakhani, V. Bikdeloo, Food Anal. Methods 9, 528–536 (2016)CrossRefGoogle Scholar
  5. 5.
    R. Xu, H.K. Lee, J. Chromatogr. A 1350, 15–22 (2014)CrossRefGoogle Scholar
  6. 6.
    C.L. Arthur, J. Pawliszyn, Anal. Chem. 62, 2145–2148 (1990)CrossRefGoogle Scholar
  7. 7.
    A.R. Ghiasvand, S. Hajipour, N. Heidari, Trends Anal. Chem. 77, 54–65 (2016)CrossRefGoogle Scholar
  8. 8.
    A. Mehdinia, M.O. Aziz-Zanjani, Trends Anal. Chem. 42, 205–215 (2013)CrossRefGoogle Scholar
  9. 9.
    A. Ghiasvand, S. Koonani, F. Yazdankhah, S. Farhadi, J. Pharm. Biomed. Anal. 149, 271–277 (2018)CrossRefGoogle Scholar
  10. 10.
    X.-Y. Song, J. Chen, Y.-P. Shi, Trends Anal. Chem. 86, 263–275 (2017)CrossRefGoogle Scholar
  11. 11.
    S. Ansari, M. Karimi, Trends Anal. Chem. 89, 146–162 (2017)CrossRefGoogle Scholar
  12. 12.
    S. Wei, W. Lin, J. Xu, Y. Wang, S. Liu, F. Zhu, Y. Liu, G. Ouyang, Anal. Chim. Acta 971, 48–54 (2017)CrossRefGoogle Scholar
  13. 13.
    M. Farjaminezhad, M.S. Tehrani, P. Aberoomand, S. Bohlooli, S.W. Hussain, J. Sep. Sci. 40, 717–724 (2017)CrossRefGoogle Scholar
  14. 14.
    H. Lan, T. Rönkkö, J. Parshintsev, K. Hartonen, N. Gan, M. Sakeye, J. Sarfraz, M.-L. Riekkola, J. Chromatogr. A 1486, 76–85 (2017)CrossRefGoogle Scholar
  15. 15.
    M. Pei, Z. Zhang, X. Huang, Y. Wu, Talanta 165, 152–160 (2017)Google Scholar
  16. 16.
    J. Wu, J. Pawliszyn, Anal. Chim. Acta 520, 257–264 (2004)CrossRefGoogle Scholar
  17. 17.
    A.R. Ghiasvand, A. Nasirian, S. Koonani, K. Nouriasl, Biomed. Chromatogr. 31, e4024 (2017)CrossRefGoogle Scholar
  18. 18.
    H. Ebrahimzadeh, A. Mehdinia, F. Kamarei, E. Moradi, Chromatographia 75, 149–155 (2012)CrossRefGoogle Scholar
  19. 19.
    L. Chen, W. Chen, C. Ma, D. Du, X. Chen, Talanta. 84, 104–108 (2011)CrossRefGoogle Scholar
  20. 20.
    J. Zou, X. Song, J. Ji, W. Xu, J. Chen, Y. Jiang, Y. Wang, X. Chen, J. Sep. Sci. 34, 2765–2772 (2011)CrossRefGoogle Scholar
  21. 21.
    M. Shamsayei, Y. Yamini, H. Asiabi, M. Rezazadeh, S. Seidi, Microchim. Acta 184, 2697–2705 (2017)CrossRefGoogle Scholar
  22. 22.
    K. Molaei, H. Bagheri, A.A. Asgharinezhad, H. Ebrahimzadeh, M. Shamsipur, Talanta 167, 607–616 (2017)CrossRefGoogle Scholar
  23. 23.
    M.F. Attia, T. Azib, Z. Salmi, A. Singh, P. Decorse, N. Battaglini, H. Lecoq, M. Omastová, A.A. Higazy, A.M. Elshafei, J. Colloid Interface Sci. 393, 130–137 (2013)CrossRefGoogle Scholar
  24. 24.
    D. Muñoz-Rojas, J. Oró-Solé, O. Ayyad, P. Gómez-Romero, Small 4, 1301–1306 (2008)CrossRefGoogle Scholar
  25. 25.
    A. Mollahosseini, E. Noroozian, Anal. Chim. Acta 638, 169–174 (2009)CrossRefGoogle Scholar
  26. 26.
    P.-C. Hsieh, C.-L. Lee, J.-F. Jen, K.-C. Chang, Analyst 140, 1275–1280 (2015)CrossRefGoogle Scholar
  27. 27.
    A. Ghiasvand, A. Nasirian, S. Koonani, K. Nouriasl, Anal. Bioanal. Chem. Res. 4, 105–126 (2017)Google Scholar
  28. 28.
    Y. Xu, W. Fan, M.C. Qian, J. Agric. Food Chem. 55, 3051–3057 (2007)CrossRefGoogle Scholar
  29. 29.
    C.L. Rainey, D.E. Bors, J.V. Goodpaster, Anal. Chem. 86, 11319–11325 (2014)CrossRefGoogle Scholar
  30. 30.
    E. Boyaci, J. Pawliszyn, Anal. Chem. 86, 8916–8921 (2014)CrossRefGoogle Scholar
  31. 31.
    A.R. Fakhari, A. Sahragard, H. Ahmar, H. Tabani, J. Electroanal. Chem. 747, 12–19 (2015)CrossRefGoogle Scholar
  32. 32.
    C.-H. Hung, H.-P. Ho, M.-T. Lin, C.-Y. Chen, Y.-Y. Shu, M.-R. Lee, J. Chromatogr. A 1265, 1–6 (2012)CrossRefGoogle Scholar
  33. 33.
    X. Cheng, H. Yan, X. Wang, N. Sun, X. Qiao, Food Chem. 162, 104–109 (2014)CrossRefGoogle Scholar
  34. 34.
    A.R. Ghiasvand, M. Pirdadeh-Beiranvand, Anal. Chim. Acta 900, 56–66 (2015)CrossRefGoogle Scholar
  35. 35.
    M. Beiranvand, A. Ghiasvand, Chromatographia 80, 1771–1780 (2017)CrossRefGoogle Scholar
  36. 36.
    J. Zeng, J. Chen, X. Song, Y. Wang, J. Ha, X. Chen, X. Wang, J. Chromatogr. A 1217, 1735–1741 (2010)CrossRefGoogle Scholar
  37. 37.
    T.Y. Tan, C. Basheer, M.J.Y. Ang, H.K. Lee, J. Chromatogr. A 1297, 12–16 (2013)CrossRefGoogle Scholar
  38. 38.
    A. Mousa, C. Basheer, A.R. Al-Arfaj, Talanta 115, 308–313 (2013)CrossRefGoogle Scholar
  39. 39.
    N.A. Chowdhury, A.M. Al-Jumaily, Wound Med. 14, 16–18 (2016)CrossRefGoogle Scholar
  40. 40.
    K. Shameli, M. Bin Ahmad, S.D. Jazayeri, S. Sedaghat, P. Shabanzadeh, H. Jahangirian, M. Mahdavi, Y. Abdollahi, Int. J. Mol. Sci. 13, 6639–6650 (2012)CrossRefGoogle Scholar
  41. 41.
    D. Djozan, T. Baheri, M. Pournaghi-Azar, Chromatographia 65, 45–50 (2007)CrossRefGoogle Scholar
  42. 42.
    R.H. Thomson, Q. Rev. Chem. Soc. 10, 27–43 (1956)CrossRefGoogle Scholar
  43. 43.
    C. Turek, F.C. Stintzing, Compr. Rev. Food Sci. Food Saf. 12, 40–53 (2013)CrossRefGoogle Scholar
  44. 44.
    H. Lord, J. Pawliszyn, J. Chromatogr. A 885, 153–193 (2000)CrossRefGoogle Scholar
  45. 45.
    A. Ghiasvand, S. Dowlatshah, N. Nouraei, N. Heidari, F. Yazdankhah, J. Chromatogr. A 1406, 87–93 (2015)CrossRefGoogle Scholar
  46. 46.
    H. Hajimehdipoor, M. Shekarchi, M. Khanavi, N. Adib, M. Amri, Pharmacogn. Mag. 6, 154 (2010)CrossRefGoogle Scholar
  47. 47.
    V. Kiyanpour, A.R. Fakhari, R. Alizadeh, B. Asghari, M. Jalali-Heravi, Talanta 79, 695–699 (2009)CrossRefGoogle Scholar
  48. 48.
    M. Rashidipour, R. Heydari, A. Feizbakhsh and P. Hashemi, ‎Nat. Prod. Res. 29, 621–627 (2015)CrossRefGoogle Scholar

Copyright information

© Iranian Chemical Society 2018

Authors and Affiliations

  1. 1.Department of ChemistryLorestan UniversityKhoramabadIran
  2. 2.Australian Centre for Research on Separation Science, School of ChemistryUniversity of TasmaniaHobartAustralia

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