Advertisement

Antimicrobial activity of endophytic fungi isolated from the mangrove plant Sonneratia apetala (Buch.-Ham) from the Sundarbans mangrove forest

  • 13 Accesses

Abstract

Endophytic fungi reside in the intercellular space of plant nourished by the plant. In return, they provide bioactive molecules which can play critical roles on plant defense system. Fifty six endophytes were isolated from the leaves, root, bark and fruits of Sonneratia apetala, a pioneer mangrove plant in the Sundarbans, Bangladesh. A total of 56 isolates were obtained and 12 different species within 8 genera were identified using morphological and molecular characteristics. Antimicrobial activity of ethyl acetate (EtOAc) and methanolic (MeOH) extracts of these 12 different species were analyzed by resazurin assay and the minimum inhibitory concentrations (MICs) were determined. The fungal extracts showed antimicrobial activities against more than one tested bacterium or fungus among 5 human pathogenic microbes, i.e. Escherichia coli NCTC 12241, Staphylococcus aureus NCTC 12981, Micrococcus lutus NCTC 7508, Pseudomonas aeruginosa NCTC 7508 and Candida albicans ATCC 90028. Overall, methanolic extracts showed greater activity than that of ethyl acetate extracts. Of the isolates identified, Colletotrichum gloeosporioides, Aspergillus niger and Fusarium equiseti were the most active isolates and showed activity against microorganisms under investigation. Methanolic extracts of C. gloeosporioides and A. niger showed the lowest MIC (0.0024 mg/mL) against P. aeruginosa. The study indicates that endophytic fungi isolated from S. apetala species possess potential antimicrobial properties, which could be further investigated.

This is a preview of subscription content, log in to check access.

Access options

Buy single article

Instant unlimited access to the full article PDF.

US$ 39.95

Price includes VAT for USA

Subscribe to journal

Immediate online access to all issues from 2019. Subscription will auto renew annually.

US$ 99

This is the net price. Taxes to be calculated in checkout.

Fig. 1
Fig. 2
Fig. 3

References

  1. Bhardwaj A, Sharma D, Jadon N, Agrawal PK (2015) Antimicrobial and phytochemical screening of endophytic fungi isolated from spikes of Pinus roxburghii. Arch Clin Microbiol 6(3):1–9

  2. Bhatia R, Narain JP (2010) The growing challenge of antimicrobial resistance in the South-East Asia Region—are we losing the battle? Ind J Med Res 135(5):482–486. https://doi.org/10.4103/0971-5916.73313

  3. Buatong J, Phongpaichit S, Rukachaisirikul V, Sakayaroj J (2011) Antimicrobial activity of crude extracts from mangrove fungal endophytes. World J Microbiol Biotechnol 27(12):3005–3008

  4. Ciardo DE, Schar G, Bottger EC, Altwegg M, Bosshard PP (2006) Internal transcribed spacer sequencing versus biochemical profiling for identification of medically important yeasts. J Clin Microbiol 44(1):77–84. https://doi.org/10.1128/JCM.44.1.77-84.2006

  5. Crouch JA, Clarke BB, White JW, Hillman BI (2009) Systematic analysis of the falcate-spored graminicolous Colletotrichum and a description of six new species from warm-season grasses. Mycologia 101:717–732. https://doi.org/10.3852/08-230

  6. Duke NC, Benzie JAH, Goodall JA, Ballmen TR (1998) Genetic structure and evolution of species in the mangrove genus Avicennia (Avicenniaceae) in the indo-west pacific. Evolution 52(6):1612–1626. https://doi.org/10.1111/j.1558-5646.1998.tb02242.x

  7. Gong LJ, Guo SX (2009) Endophytic fungi from Dracaena cambodiana and Aquilaria sinensis and their antimicrobial activity. Afr J Biotechnol 8(5):731–736

  8. Hormazabal E, Piontelli E (2009) Endophytic fungi from Chilean native gymnosperms: antimicrobial activity against human and phytopathogenic fungi. World J Microbiol Biotechnol 25:813–819. https://doi.org/10.1007/s11274-008-9953-6

  9. Jalgaonwala R, Mohite B, Mahajan RA (2011) Review: natural products from plant associated endophytic fungi. J Microbiol Biotechnol Res 1:21–32

  10. Lv YL, Zhang FS, Chen J, Cui JL, Xing YM, Li XD, Guo SX (2010) Diversity and antimicrobial activity of endophytic fungi associated with the alpine plant Saussurea involucrata. Biol Pharm Bull 33(8):1300–1306. https://doi.org/10.1248/bpb.33.1300

  11. Artika IM, Julistiono H, Bermawie N, Riyanti EI, Hasan AEZ (2017) Anticancer activity test of ethyl acetate extract of endophytic fungi isolated from soursop leaf (Annona muricata L.). Asian Pac J Trop Med 10(6):566–571

  12. Morens DM, Folkers GK, Fauci AS (2004) The challenge of emerging and reemerging infectious diseases. Nature 430:242–249. https://doi.org/10.1038/nature02759

  13. Nurunnabi TR, Nahar L, Al-Majmaie S et al (2018) Anti-MRSA activity of oxysporone and xylitol from the endophytic fungus Pestalotia sp. growing on the Sundarbans mangrove plant Heritiera fomes. Phytother Res 32(2):348–354. https://doi.org/10.1002/ptr.5983

  14. Qadri M, Johri S, Shah BA, Khajuria A, Sidiq T, Lattoo SK, Abdin MZ, Riyaz-Ul-Hassan S (2013) Identification and bioactive potential of endophytic fungi isolated from selected plants of the Western Himalayas. Springer Plus 2:1–14. https://doi.org/10.1186/2193-1801-2-8

  15. Rahmawati NI, Sunarva S, Rumidatul S (2018) Exploration of potential bioactive compounds of endophytic microbial culture isolated from gall rust sengon (Falcataria moluccana). J Pharm Res Sci 10(11):156–169

  16. Sarker SD, Nahar L, Kumarasamy Y (2007) Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods 42:321–324. https://doi.org/10.1016/j.ymeth.2007.01.006

  17. Sette L, Passarini M, Delarmelina C, Salati F, Duarte M (2006) Molecular characterization and antimicrobial activity of endophytic fungi from coffee plants. World J Microbiol Biotechnol 22(11):1185–1195. https://doi.org/10.1007/s11274-006-9160-2

  18. Tomlinson PB (1986) The botany of mangroves. Cambridge University Press, Cambridge

  19. Xing XK, Chen J, Xu MJ, Lin WH, Guo SX (2011) Fungal endophytes associated with Sonneratia (Sonneratiaceae) mangrove plants on the south coast of China. Forest Pathol 41:334–340. https://doi.org/10.1111/j.1439-0329.2010.00683.x

  20. Zabala NQ (1990) Silviculture of species. Development of professional education in the Forestry Sector of Bangladesh. Institute of Forestry, Chittagong University, Hathazari, pp 96–150

Download references

Acknowledgements

The research was funded by Robert S. McNamara Fellowship Programme from the World Bank.

Author information

Correspondence to Md. Morsaline Billah.

Ethics declarations

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

Tauhidur R. Nurunnabi has no conflict of interest. Farah Sabrin has no conflict of interest. Dilara I. Sharif has no conflict of interest. Lutfun Nahar has no conflict of interest. Md. H. Sohrab has no conflict of interest. Satyajit D. Sarker has no conflict of interest. S. M. Mahbubur Rahman has no conflict of interest. Md. Morsaline Billah has no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Nurunnabi, T.R., Sabrin, F., Sharif, D.I. et al. Antimicrobial activity of endophytic fungi isolated from the mangrove plant Sonneratia apetala (Buch.-Ham) from the Sundarbans mangrove forest. ADV TRADIT MED (ADTM) (2020) doi:10.1007/s13596-019-00422-9

Download citation

Keywords

  • Antimicrobial
  • Endophytic fungi
  • Mangrove plants
  • Sonneratia apetala
  • The sundarbans