Antimicrobial activity of endophytic fungi isolated from the mangrove plant Sonneratia apetala (Buch.-Ham) from the Sundarbans mangrove forest

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Endophytic fungi reside in the intercellular space of plant nourished by the plant. In return, they provide bioactive molecules which can play critical roles on plant defense system. Fifty six endophytes were isolated from the leaves, root, bark and fruits of Sonneratia apetala, a pioneer mangrove plant in the Sundarbans, Bangladesh. A total of 56 isolates were obtained and 12 different species within 8 genera were identified using morphological and molecular characteristics. Antimicrobial activity of ethyl acetate (EtOAc) and methanolic (MeOH) extracts of these 12 different species were analyzed by resazurin assay and the minimum inhibitory concentrations (MICs) were determined. The fungal extracts showed antimicrobial activities against more than one tested bacterium or fungus among 5 human pathogenic microbes, i.e. Escherichia coli NCTC 12241, Staphylococcus aureus NCTC 12981, Micrococcus lutus NCTC 7508, Pseudomonas aeruginosa NCTC 7508 and Candida albicans ATCC 90028. Overall, methanolic extracts showed greater activity than that of ethyl acetate extracts. Of the isolates identified, Colletotrichum gloeosporioides, Aspergillus niger and Fusarium equiseti were the most active isolates and showed activity against microorganisms under investigation. Methanolic extracts of C. gloeosporioides and A. niger showed the lowest MIC (0.0024 mg/mL) against P. aeruginosa. The study indicates that endophytic fungi isolated from S. apetala species possess potential antimicrobial properties, which could be further investigated.

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The research was funded by Robert S. McNamara Fellowship Programme from the World Bank.

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Correspondence to Md. Morsaline Billah.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Tauhidur R. Nurunnabi has no conflict of interest. Farah Sabrin has no conflict of interest. Dilara I. Sharif has no conflict of interest. Lutfun Nahar has no conflict of interest. Md. H. Sohrab has no conflict of interest. Satyajit D. Sarker has no conflict of interest. S. M. Mahbubur Rahman has no conflict of interest. Md. Morsaline Billah has no conflict of interest.

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Nurunnabi, T.R., Sabrin, F., Sharif, D.I. et al. Antimicrobial activity of endophytic fungi isolated from the mangrove plant Sonneratia apetala (Buch.-Ham) from the Sundarbans mangrove forest. ADV TRADIT MED (ADTM) (2020) doi:10.1007/s13596-019-00422-9

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  • Antimicrobial
  • Endophytic fungi
  • Mangrove plants
  • Sonneratia apetala
  • The sundarbans