Cannabis sativa (C. sativa) is a widespread medicinal plant used for humans and cattles infectious diseases. The current study is carried out to assess phytochemical, antimicrobial, antioxidant, α-amylase inhibition and cytotoxic activity of the Cannabis sativa plant. An appropriate amount of phytochemicals like tannins, flavonoids, lycopene, and β-carotenes is found in C. sativa plant during the phytochemical screening. Among these phytochemicals of C. sativa plant, tannin shows outstanding activity. The plant extracts are assessed for antimicrobial activity against different seven bacterial strains. Notably, the stem part of C. sativa is found to exhibit higher antimicrobial potential than root and leaf. Among the extracts, the antioxidant assays (evaluated through % inhibition of linoleic acid peroxidation assessment) of n-hexane extract of leaf part was highest (80.70%) compared to the others. The plant also contains a potent α-amylase inhibitory potential, the root extract shows maximum inhibition of α-amylase. The cytotoxic effect [evaluated through hemolytic activity as (%) lysis of RBCs against human erythrocytes] of the plant extracts is found insignificant ranging from 1.97 to 5.88. The plant C. sativa keeps a significant amount of bioactive phenolic combinations accompanied by high antimicrobial and antioxidant activities.
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This work was supported by the National Natural Science Foundation of China Grant No. 51950410596. However the practical work was performed at Department of Biochemistry, Abdul Wali Khan University Mardan, Pakistan.
Compliance with ethical standards
This article does not contain any studies with human participants or animals performed by any of the authors.
Conflict of interest
Syed Bilal Shah has no conflict of interest. Lubna Sartaj has no conflict of interest. Shahid Hussain has no conflict of interest. Nabi Ullah has no conflict of interest. Muhammad Idrees has no conflict of interest. Asma Shaheen has no conflict of interest. Muhammad Sufyan Javed has no conflict of interest. Muhammad Kashif Aslam has no conflict of interest.
Ahmad I, Beg AZ (2001) Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. J Ethnopharmacol 74:113–123CrossRefGoogle Scholar
Ammer MR, Zaman S, Khalid M et al (2016) Optimization of antibacterial activity of Eucalyptus tereticornis leaf extracts against Escherichia coli through response surface methodology. J Radiat Res Appl Sci 9:376–385CrossRefGoogle Scholar
Ashraf MW, Bilal M, Iqbal M (2014) Allium sativum aqueous extract inhibitory effect on advanced glycation end product. Int J Clin Biol Sci 4:38–44Google Scholar
Chanda SV, Kaneria MJ (2012) Optimization of conditions for the extraction of antioxidants from leaves of Syzygium cumini L. using different solvents. Food Anal Methods 5:332–338CrossRefGoogle Scholar
Chen WC, Ho CT (1995) Antixoidant properties of polyphenols extracted from green and black teas. J Food Lipids 2:35–46CrossRefGoogle Scholar
Johns TE, Eyzaguirre PB (2006) Linking biodiversity, diet and health in policy and practice. Proc Nutr Soc 65:182–189CrossRefGoogle Scholar
Khan H, Saeed M, Muhammad N et al (2013) Phytochemical analysis, antibacterial and antifungal assessment of aerial parts of polygonatum verticillatum. Toxicol Ind Health 32:841–847CrossRefGoogle Scholar
Lillian B, Maria JF, Bruno Q et al (2007) Total phenols, ascorbic acid, β-caroteneand lycopene in Portuguese wild edible mushrooms and their antioxidant activities. Food Chem 103:413–419CrossRefGoogle Scholar
Majid A, Rahman MU, Shah JA et al (2013) In vitro antibacterial activity of Camellia sinensis leaf extracts to some selective pathogenic bacterial strains. Int J Biosci 3:69–75CrossRefGoogle Scholar
Makkar HPS, Bluemmel M, Borowy NK et al (1993) Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods. J Sci Food Agric 61:161–165CrossRefGoogle Scholar
Molchanova N, Hansen PR, Franzyk H (2017) Advances in development of antimicrobial peptidomimetics as potential drugs. Molecules 22:1430CrossRefGoogle Scholar
Mothana RA, Abdo SA, Hasson S et al (2010) Antimicrobial, antioxidant and cytotoxic activities and phytochemical screening of some yemeni medicinal plants. Evid Based Complement Altern Med 7:323–330CrossRefGoogle Scholar
Naveed M, Khan TA, Ali I et al (2014) In vitro antibacterial activity of Cannabis sativa leaf extracts to some selective pathogenicbacterial strains. Int J Biosci 4:65–70Google Scholar
Powell WA, Catranis CM, Maynard CA (2000) Design of self-processing antimicrobial peptides for plant protection. Lett Appl Microbiol 31:163–168CrossRefGoogle Scholar
Radwan MM, Elsohly MA, Slade D (2009) Biologically active cannabinoids from high-potency Cannabis sativa. J Nat Prod 72:906–911CrossRefGoogle Scholar
Riaz M, Rasool N, Bukhari IH et al (2012) In vitro antimicrobial, antioxidant, cytotoxicity and GC–MS analysis of Mazus goodenifolius. Molecules 17:14275–14287CrossRefGoogle Scholar
Rosales GR (2002) Carotenoid and fruit development effects on germination and vigor of tomato (Lycopersicon esculentum Mill.) seeds. Ph.D Thesis, The Ohio State University, Horticulture and Crop ScienceGoogle Scholar
Sachindra N, Pradhan A (1977) Marijuana drug abuse clinical and basic aspects. C.V. Mosby Company, Saint Louis, pp 148–173Google Scholar
Satyal P, Setzer WN (2014) Chemotyping and determination of antimicrobial, insecticidal and cytotoxic properties of wild-grown cannabis sativa from Nepal. J Med Act Plants 3:1–4Google Scholar
Shah SB, Parveen Z, Bilal M et al (2018) Assessment of antimicrobial, antioxid ant and cytotoxicity properties of Camellia sinensis L. Pak J Pharm Sci 31:1285–1291PubMedGoogle Scholar
Sharma P, Sharma JD (2001) In vitro hemolysis of human erythrocytes by plant extracts with antiplasmodial activity. J Ethnopharmacol 74:239–243CrossRefGoogle Scholar
Wasim K, Haq I, Ashraf M (1995) Antimicrobial studies of the leaf of Cannabis sativa L. Pak J Pharm Sci 8:22–38Google Scholar
Whittaker K, Roth MD, Salehi K et al (2004) Mechanisms for impaired effector function in alveolar macrophages from marijuana and cocaine smokers. J Neuroimmunol 147:82–86CrossRefGoogle Scholar
Xiao Z, Storms R, Tsang A (2006) A quantitative starch-iodine method for measuring alpha-amylase and glucoamylase activities. Anal Biochem 351:146–148CrossRefGoogle Scholar
Zakir M, Sultan KB, Khan H et al (2015) Antimicrobial activity of different tea varieties available in Pakistan. Pak J Pharm Sci 28:2091–2094PubMedGoogle Scholar