In-vitro evaluation of antimicrobial, antioxidant, alpha-amylase inhibition and cytotoxicity properties of Cannabis sativa

  • Syed Bilal Shah
  • Lubna Sartaj
  • Shahid HussainEmail author
  • Nabi Ullah
  • Muhammad Idrees
  • Asma Shaheen
  • Muhammad Sufyan Javed
  • Muhammad Kashif Aslam
Research Article


Cannabis sativa (C. sativa) is a widespread medicinal plant used for humans and cattles infectious diseases. The current study is carried out to assess phytochemical, antimicrobial, antioxidant, α-amylase inhibition and cytotoxic activity of the Cannabis sativa plant. An appropriate amount of phytochemicals like tannins, flavonoids, lycopene, and β-carotenes is found in C. sativa plant during the phytochemical screening. Among these phytochemicals of C. sativa plant, tannin shows outstanding activity. The plant extracts are assessed for antimicrobial activity against different seven bacterial strains. Notably, the stem part of C. sativa is found to exhibit higher antimicrobial potential than root and leaf. Among the extracts, the antioxidant assays (evaluated through % inhibition of linoleic acid peroxidation assessment) of n-hexane extract of leaf part was highest (80.70%) compared to the others. The plant also contains a potent α-amylase inhibitory potential, the root extract shows maximum inhibition of α-amylase. The cytotoxic effect [evaluated through hemolytic activity as (%) lysis of RBCs against human erythrocytes] of the plant extracts is found insignificant ranging from 1.97 to 5.88. The plant C. sativa keeps a significant amount of bioactive phenolic combinations accompanied by high antimicrobial and antioxidant activities.


Cannabis sativa Phytochemicals α-Amylase Antioxidant Antimicrobial Cytotoxicity 


C. sativa

Cannabis sativa


Optical density


Cardiovascular diseases



This work was supported by the National Natural Science Foundation of China Grant No. 51950410596. However the practical work was performed at Department of Biochemistry, Abdul Wali Khan University Mardan, Pakistan.

Compliance with ethical standards

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

Syed Bilal Shah has no conflict of interest. Lubna Sartaj has no conflict of interest. Shahid Hussain has no conflict of interest. Nabi Ullah has no conflict of interest. Muhammad Idrees has no conflict of interest. Asma Shaheen has no conflict of interest. Muhammad Sufyan Javed has no conflict of interest. Muhammad Kashif Aslam has no conflict of interest.


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Copyright information

© Institute of Korean Medicine, Kyung Hee University 2019

Authors and Affiliations

  1. 1.School of Materials Science and EngineeringJiangsu UniversityZhenjiangPeople’s Republic of China
  2. 2.State Key Laboratory of Microbial Metabolism, School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghaiPeople’s Republic of China
  3. 3.Department of BiochemistryAbdul Wali Khan University MardanMardanPakistan
  4. 4.Department of Earth SciencesUniversity of SargodhaSargodhaPakistan
  5. 5.Siyuan Laboratory, Department of PhysicsJinan UniversityGuangzhouPeople’s Republic of China
  6. 6.Institute for Clean Energy and Advanced Materials, School of Materials and EnergySouthwest UniversityChongqingPeople’s Republic of China

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