Anti-hypernociceptive effects of methanol extract of Boswellia dalzielii on STZ-induced diabetic neuropathic pain
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Painful neuropathy that can cause hyperalgesia and allodynia is the most common and debilitating complication of diabetes. Both hyperglycemia and oxidative stress clearly play a key role in the progression of diabetic neuropathy. The search for new natural products with combined anti-inflammatory, antioxidant, hypoglycemic and antinociceptive properties is important in the treatment of this disease. The purpose of this study was to evaluate the anti-hypernoceptive effect of the methanolic extract of Boswellia dalzielii in streptozotocin-induced diabetic mice. Thus, the mice were injected with 200 mg/kg of streptozotocin to produce hyperglycemia. Von Frey filaments, hotplate, acetone and formalin tests were used to evaluate anti-nociceptive activity. Assays of catalase, SOD, MDA and NO determined the antioxidant properties. When administered, methanolic extract of Boswellia dalzielii (250 and 500 mg/kg, p.o.) significantly reduced licking/biting behavior during the first and second phase of the formalin test in a dose-dependent manner. Moreover, this extract significantly improved the glycemia of diabetic mice, it also significantly decreased the levels of TNF alpha, IL-1 beta in the serum and the sciatic nerve, decreased the levels of MDA and NO, and then increased the activity of SOD and catalase enzymes. Our results suggest that methanolic extract of Boswellia dalzielii can be a useful therapeutic agent, presumably for both prevention and reversal of pathophysiologic pain. This effect seems to imply hyperglycemic and antioxidant properties of this plant.
KeywordsBoswellia dalzielii Neuropathic pain Diabetic pain Streptozotocin
The authors would like to thank the study participants; the staff of Department of Pharmacy, Comsats University Islamabad, Abbottabad campus, Abbottabad-22060, Pakistan. The authors wish to express their gratitude to TWAS (Academy of Science of Developing Countries) and Comsats University Islamabad, Abbottabad campus staff member.
MM and KR designed the work. MM, KR, MA, NIS, HD, NUR and IA conducted the work, collected and analysed the data. MM, AAD, AG and NYW drafted the manuscript and revised it critically. All authors agree to be accountable for all aspects of the work.
This manuscript research project was supported by the TWAS (Academy of Science of Developing Countries) and the Comsats University Islamabad, Abbottabad campus, Pakistan, under the Postdoctoral Fellowship Award to Mbiantcha Marius (RF No: 3240287152).
Compliance with ethical standards
To demonstrate the coherent effects of our different compounds, the minimum possible of animals as the intensity of nociceptive stimuli was used. All tests were achieved using mature male and female mice (3 months old; 25–35 g), bred in the animal house facility (controlled temperature (22 ± 1 °C); 12 h light/12 h dark cycle with standard lab chow and water ad libitum) of the National Institute of Health (NIH), Islamabad, Pakistan. The treatment of animals was in agreement with the Institutional Animal Care and Use Committee (IACUC) of the National Institute of Health, and the study protocols accepted by the ethics committee of National Institute of Health, Islamabad, Pakistan (NIH publication no. 85-23, revised 1985).
Conflict of interest
Marius Mbiantcha has no conflict of interest. Rauf Khalid has no conflict of interest. Donatien Albert Atsamo has no conflict of interest. Isaac S. Njoku has no conflict of interest. Arif Mehreen has no conflict of interest. Gilbert Ateufack has no conflict of interest. Dar Hamza has no conflict of interest. William Yousseu Nana has no conflict of interest. Rehman Ur Naeem has no conflict of interest. Ahmad Izhar has no conflict of interest.
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