Analgesic activity of flavonoids isolated from Persicaria glabra (wild)

  • Manivannan RajamanickamEmail author
  • Shopna Rajamohan
Research Article


The present experimental study conducted to determine the four flavonoids (1–4) isolated from extracts of Persicaria glabra (Polygonaceae) and was tested for its potential central and peripheral analgesic activity. All animals were lowered on a hot plate (55 ± 0.5 °C), the observations were made before and after administration of respective drugs at 30, 60, and at the end of 90 min. In writhing method, acetic acid is administered intraperitoneal to the experimental animals to create pain sensation. The writhing movements were observed and counted for 30 min after acetic acid administration. The results showed that the compound quercetin (1) and its glycosides (3) at a dose of 100 and 200 mg/kg body weight significant analgesic activity (P < 0.05) in both central and peripheral models of analgesia used. The same doses, compounds 2 and 4 did not show any analgesic effects. Among them, quercetin (1) was the most potent in both models tested. Also due to structural difference of four compounds, a number of hydroxyl groups, substitution of –OCH3 and glycosylation, they will exhibit different analgesic responses. The P. glabra leaf extracts containing mixture of quercetin, isorhamnetin and their glycosides were effective in both the central and peripheral models of pain.


Persicaria glabra Analgesic activity Isolated flavonoids 



Authors are thankful to Department of Chemistry, Botany and Zoology, Government Arts College (Autonomous), Kumbakonam for providing all the required research facilities.

Compliance with ethical standards

Ethical statement

The welfare of animals used for analgesic activity (research) of the test compounds was evaluated in male albino mice (25–30 g) approved by Institutional Animal Ethics Committee (IAEC), Bharathidasan University, Trichirappalli, Tamilnadu, India (Approval No. BDU/IAEC/2011/31/29.03.2011).

Conflict of interest

Manivannan Rajamanickam has no conflict of interest. Shopna Rajamohan has no conflict of interest.


  1. Agarwal PK (1989) Carbon-13 NMR of flavonoids. Elsevier, New York, pp 95–175CrossRefGoogle Scholar
  2. Barr GA (1999) Antinociceptive effects of locally administered morphine in infant rats. Pain 81:155–161CrossRefGoogle Scholar
  3. Boulos L, Hadidi MN, Gohary M (1984) The weed flora of Egypt. American University in Cairo Press, CairoGoogle Scholar
  4. Byoung JP, Tomohiko M, Tsutomu K, Cheol HP, Kwang JC, Michio O (2012) Phenolic compounds from the leaves of Psidium guajava II. Quercetin and its glycosides. Chem Nat Comp 48(3):477–479CrossRefGoogle Scholar
  5. Dai J, Mumper RJ (2010) Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 15:7313–7352CrossRefGoogle Scholar
  6. Eddy NB, Leimback D (1953) Synthetic analgesic. II. Dithienyl butenylanddithienyl butyl amines. J Pharmacol Exper Therap 107:385–393Google Scholar
  7. Gamble JS, Fischer CEC (1957) Flora of the presidency of madras. Newman and Adlard, London (Reprint ed. Vol. II. Botanical Survey of India, Calcutta) 1925, p 1244Google Scholar
  8. Harborne JB, Baxter H (1999) The handbook of natural flavonoids, vol 1,2. Wiley, ChichesterGoogle Scholar
  9. Harborne JB, Williams CA (2000) Advances in flavonoid research since 1992. Phytochemistry 55:481–504CrossRefGoogle Scholar
  10. Hasselstrom J, Liu-Palmgren J, Rasjo-Wraak G (2002) Prevalence of pain in general practice. Eur J Pain 6:375–385CrossRefGoogle Scholar
  11. Havsteen B (1983) Flavonoids, a class of natural products of high pharmacological potency. Biochem Pharmacol 32:1141–1148CrossRefGoogle Scholar
  12. Heim KE, Tagliaferro AR, Bobilya DJ (2002) Flavonoid antioxidants: chemistry, metabolism and structure–activity relationships. J Nutr Biochem 13:572–584CrossRefGoogle Scholar
  13. Hossain MM, Ali MS, Saha A, Alimuzzaman M (2006) Antinociceptive activity of whole plant extracts of Paederiafoetida. Dhaka Univ J Pharm Sci 5:67–69CrossRefGoogle Scholar
  14. Jain PS, Mallipedi S, Belsare DP, Mandal SC, Pal SC, Badgujar VB (2007) Analgesic activity of stem bark of Kigelia pinnata Linn. Indian Drugs 44:63Google Scholar
  15. Kim GB, Shin KS, Kim CM, Kwon YS (2006) Flavonoids from the leaves of Rhododendron schlipenbachi. Kor J Pharmacog 37:177–183Google Scholar
  16. Koster R, Anderson M, De Beer EJ (1959) Acetic acid for analgesics screening. Fed Proc 18:412–417Google Scholar
  17. Kulkarni SK (1999) Handbook of experimental pharmacology, 2nd edn. Vallabh Prakashan, Delhi, pp 123–128Google Scholar
  18. Kumarasamy Y, Nahar L, Byres M, Delazar A, Sarker SD (2005) The assessment of biological activities associated with the major constituents of the methanol extracts of wild carrot (Daucus carota L) seeds. J Herb Pharmacother 5(1):61–72PubMedGoogle Scholar
  19. Lin HT, Nah SL, Huang YY, Wu SC (2010) Potential antioxidant components and characteristics of fresh Polygonum multiflorum. J Food Drug Anal 18:120–127Google Scholar
  20. Litchfield JT, Wilcoxon F (1949) A simplified method of evaluating dose-effect experiments. J Pharmacol Exper Therap 96:99–133Google Scholar
  21. Mabry TJ, Markham KR, Thomas MB (1970) The systematic identification of flavonoids. Springer, BerlinCrossRefGoogle Scholar
  22. Manivannan R, Shopna R (2015) Isolation of quercetin and isorhamnetin derivatives and evaluation of anti-microbial and anti-inflammatory activities of Persicaria glabra. Nat Prod Sci 21(3):170–175Google Scholar
  23. Mantyselka P, Kumpusalo E, Ahonen R, Kumpusalo A, Kauhanen J, Viinamaki H, Halonen P, Takala J (2001) Pain as a reason to visit the doctor: a study in Finnish primary health care. Pain 89:175–180CrossRefGoogle Scholar
  24. Markham KR, Geiger H (1994) 1H nuclear magnetic resonance spectroscopy of flavonoids and their glycosides in hexadeutero dimethyl sulfoxide. In: Harborne JB (ed) Advances in research since 1986. Chapman and Hall, London, pp 441–497Google Scholar
  25. Middleton JE, Chithan K (1993) The impact of plant flavonoids on mammalian biology: implications for immunity, inflammation and cancer. In: Harborne JB (ed) The flavonoids: advances in research since 1986. Chapman and Hall, LondonGoogle Scholar
  26. Mittal DK, Joshi D, Shukla S (2011) Hepatoprotective effects of Polygonum bistorta (Linn.) and its active compound against acetaminophen-induced toxicity in rats. Toxicol Lett 205:S237CrossRefGoogle Scholar
  27. Muddathir AK, Balansard G, Timon-David P, Babadjamian A, Yagoub AK, Julien MJ (1987) Anthelmintic properties of Polygonum glabrum. J Pharm Pharmacol 39(4):296–300CrossRefGoogle Scholar
  28. Rastelli G, Antolini L, Benvenuti S, Constantino L (2000) Structural bases for the inhibition of aldose reductase by phenolic compounds. Bioorg Med Chem 8:1151–1158CrossRefGoogle Scholar
  29. Ronaldo AR, Mariana LV, Sara MT, Adriana BPP, Steve P (2000) Involvement of resident macrophages and mast cells in the writhing nociceptive response induced by zymosan and acetic acid in mice. Eur J Pharmacol 387:111–118CrossRefGoogle Scholar
  30. Schnitzer TJ (2006) Update on guidelines for the treatment of chronic musculoskeletal pain. Clin Rheumatol 25(Suppl 1):229Google Scholar
  31. Shiddamallayya N, Yasmeen Azra, Gopakumar K (2010) Medico-botanical survey of kumar parvatha kukke subramanya, Mangalore, Karnataka. Indian J Tradit Knowl 9(1):96–99Google Scholar
  32. Skibola CF, Smith MT (2000) Potential health impacts of excessive flavonoid intake. Free Radic Biol Med 29:375–383CrossRefGoogle Scholar

Copyright information

© Institute of Korean Medicine, Kyung Hee University 2019

Authors and Affiliations

  1. 1.Department of ChemistryGovernment Arts College (Autonomous)KumbakonamIndia

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