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Oriental Pharmacy and Experimental Medicine

, Volume 19, Issue 4, pp 445–454 | Cite as

Phytochemical screening and evaluation of pharmacological activities of aqueous extract of Typhonium trilobatum (L.) Schott

  • Tania Parvin
  • Mim Binti Akhlas
  • Fatema Khatun
  • Anzuman Akter
  • Md. Al Amin
  • Muhammad Torequl IslamEmail author
  • Razina Rouf
Research Article
  • 44 Downloads

Abstract

This study aimed at phytochemical analysis and evaluation of toxic and protective effects of the crude aqueous extract of Typhonium trilobatum. Toxicity analysis was done in Allium cepa, while anti-inflammatory, and membrane stabilizing and clotlysis capacity were tested by using egg albumin and human erythrocytes, respectively. The results suggest that T. trilobatum aqueous extract contains alkaloids, glycosides, tannins, saponins, flavonoids and reducing sugars. The crude extract exhibited toxicity in A. cepa in a time and concentration dependent manner. The highest root growth inhibition was observed at exposure time 72 h in 20% (v/v) concentration extract of the herb. The extract at low concentration (2.5 and 5%) reduced the root growth inhibition capacity at 48 h in comparison to 24 and 72 h, possibly by adaptation of DNA damage profile in the eukaryotic test system (A. cepa), which was further confirmed in anti-inflammatory and membrane stabilizing, and anti-atherothrombosis test. In the latter cases, the extract at 0.02–0.08% (v/v) significantly (p < 0.05, p < 0.01, p < 0.001) inhibited protein denaturation, membrane lysis, while enhanced clotlysis capacity in a concentration-dependent manner. In conclusion, the extract exerted toxic effects at high concentrations and protective effects at low concentrations. T. trilobatum may be one of the potential sources of phytotherapeutic agents, especially for the treatment of inflammatory and cardiovascular diseases and disorders.

Keywords

Typhonium trilobatum Toxicity Anti-inflammatory Membrane stabilization Thrombolysis 

Notes

Acknowledgements

We are thankful to the Department of Pharmacy, Banghabondhu Sheikh Mujibur Rahman Science and Technology University for providing the laboratory facilities to conduct this study. We are also owed to the Department for Management of Science and Technology Development & Faculty of Pharmacy, Ton Duc Thang University, Vietnam for funding this study.

Compliance with ethical standards

Ethical statement

This study was approved by the Ethical Committee under the Department of Pharmacy, Bangabandhu Sheikh. Mujibur Rahman Science and Technology University (BSMRSTU) (Approval No. 2014/0109031).

Conflicts of interest

Tania Parvin has no conflict of interest. Mim Binti Akhlas has no conflict of interest. Fatema Khatun has no conflict of interest. Anzuman Akter has no conflict of interest. Md. Al Amin has no conflict of interest. Muhammad Torequl Islam has no conflict of interest. Razina Rouf has no conflict of interest.

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Copyright information

© Institute of Korean Medicine, Kyung Hee University 2019

Authors and Affiliations

  1. 1.Department of PharmacyBanghabondhu Sheikh Mujibur Rahman Science and Technology UniversityGopalganjBangladesh
  2. 2.Department for Management of Science and Technology Development, Faculty of PharmacyTon Duc Thang UniversityHo Chi Minh CityVietnam
  3. 3.Faculty of PharmacyTon Duc Thang UniversityHo Chi Minh CityVietnam

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