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Phytochemical screening and evaluation of the pharmacological activities of ethanolic extract of Argemone mexicana Linn. aerial parts

  • Kishore Kumar Sarkar
  • Trina Mitra
  • Rabindra Nath Acharyya
  • Samir Kumar Sadhu
Research Article
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Abstract

The present study was designed to investigate different phytochemical groups present in ethanolic extract of Argemone mexicana aerial parts (EAMA) as well as to assess the analgesic, antidiarrheal, antibacterial, anthelmintic and cytotoxic activities of EAMA. Moreover, peripheral and central analgesic activities were evaluated by acetic acid-induced writhing test, formalin-induced paw licking test, tail immersion test and hot plate test. In vivo castor oil-induced diarrheal model and magnesium sulphate induced diarrheal model in mice were utilized for the assessment of antidiarrheal activity. Again, antibacterial activity was evaluated using disc diffusion assay. Anthelmintic activity was carried out on Paramphistomum cervi (Trematoda). Cytotoxic activity was assessed through brine shrimp lethality bioassay. The extract demonstrated the presence of alkaloids, flavonoids, phenolic compounds, tannins, glycosides and gums in phytochemical screening. In case of acetic acid-induced writhing and formalin induced paw licking test, both lower and higher doses of EAMA showed significant percentage inhibition of writhing as well as paw licking respectively (*P < 0.05, vs. control). EAMA at the doses of 200 mg/kg and 400 mg/kg revealed significant latency response (*P < 0.05, vs. control) in delayed phase in both tail immersion as well as hot plate test. Moreover, significant percentage inhibition (*P < 0.05, vs. control) of diarrhea was exposed by both doses of EAMA in in vivo diarrheal models. In disc diffusion assay, EAMA showed antibacterial activities against both gram positive and gram negative bacterial strains. Again, the extract exhibited anthelmintic and cytotoxic activity in a dose dependent manner.

Keywords

Argemone mexicana Analgesic Antidiarrheal Antibacterial Anthelmintic Cytotoxic 

Notes

Acknowledgements

The authors are grateful to the Department of Pharmacy, Jessore University of Science and Technology for providing all of the laboratory facilities, instrumental support as well as chemicals and reagents required to carry out this research work. Again, we would like to thank to the Department of Pharmacy, Jahangirnagar University, Savar, Dhaka, Bangladesh and International Centre for Diarrheal Disease and Research, Bangladesh (ICDDR, B) for supplying experimental mice and bacterial strains respectively.

Compliance with ethical standards

Ethical statement

The design of the experiments involving mice were approved by the Ethical Review Committee of Faculty of Biological Science and Technology, Jessore University of Science and Technology, Jessore-7408 [Ref: ERC/FBS/JUST/2018-4(A)].

Conflict of interest

This manuscript described has not been published before; not under consideration for publication anywhere else; and has been approved by all co-authors.

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Copyright information

© Institute of Korean Medicine, Kyung Hee University 2019

Authors and Affiliations

  • Kishore Kumar Sarkar
    • 1
  • Trina Mitra
    • 1
  • Rabindra Nath Acharyya
    • 2
  • Samir Kumar Sadhu
    • 2
  1. 1.Department of PharmacyJessore University of Science and TechnologyJessoreBangladesh
  2. 2.Pharmacy Discipline, Life Science SchoolKhulna UniversityKhulnaBangladesh

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