Quantification of the secondary metabolites by HPTLC, analgesic and antipyretic activity evaluation of Ficus racemosa L. leaves
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There have been no reports of simultaneous quantification of kaempferol and quercetin in Ficus racemosa L. Objective of the present work included to perform extraction using petroleum ether (PEE) and ethanol (EE), to perform preliminary phytochemical test, quantitative estimation of phytochemicals and simultaneous estimation of kaempferol and quercetin in Ficus racemosa L. using high performance thin layer chromatography (HPTLC) method and to assess the analgesic and antipyretic activity of the extracts. PEE and EE obtained from cold maceration technique. Extracts were subjected to qualitative test and estimated quantitatively. Extracts were applied on silica gel G 60 F254 plate; solvent-Toluene: Chloroform: Acetone 4.5:4.5:1 (v/v) and scanned at 254 nm. The extracts were also subjected to analgesic activity (hot plate method and tail immersion method) and antipyretic (Brewer’s yeast induced pyrexia model and Lipopolysaccharide induced pyrexia mode) activity. Findings of preliminary phytochemical test and quantitative estimation of phytochemicals confirms the presence of flavonoids in both the extracts (PEE and EE). The amount of flavonoids in PEE and EE were found to be 24.58 ± 0.60 mg/100 g and 21.01 ± 0.58 mg/100 g respectively. HPTLC system resulted well resolved bands for quercetin (Rf 0.38) and kaempferol (Rf 0.55). The validated HPTLC method was found suitable for kaempferol and quercetin quantification in Ficus racemosa L. Hot plate reaction time response was improved in a dose-dependent manner in a group of rats treated with PEE suggesting its analgesic potential. In view of analgesic potential by tail immersion method, PEE (400 mg/kg b.w.) showed no significant difference in tail flick reaction time as shown by standard treated animal (Aspirin 100 mg/kg b.w.). The findings of antipyretic activity suggested that PEE (400 mg/kg b.w.) have no significant difference in treating pyrexia when compared with Paracetamol (100 mg/kg). PEE (400 mg/kg b.w.) was found to be most potent. It may be due to the presence of comparatively high amount of flavonoids in PEE confirmed by the preliminary phytochemical test, quantitative estimation of phytochemicals and HPTLC quantification the PEE was found to be more effective when compared to EE.
KeywordsKaempferol Quercetin Lipopolysaccharide HPTLC quantification
Authors are thankful to the Administration of IFTM University, Moradabad for the admirable assistance and motivation to complete the lab work.
Compliance with ethical standards
The experimental protocols were approved (Ref 2016/839/ac/MPH/31) by the Institutional Animal Ethics Committee (IAEC) constituted under Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA-837/ac/2004) guidelines.
Conflict of interest
This manuscript described has not been published before; not under consideration for publication anywhere else; and has been approved by all co-author.
- Akapa TC, Kehinde AO, Beatrice OO, Olajide OJ (2014) Antipyretic activity of Abutilon mauritianum (Jacq.) roots in Wistar rats. Int J Pharm Sci Res 5(2):42–45Google Scholar
- Anonymous (1952) The wealth of India. Council of Scientific and Industrial Research, New DelhiGoogle Scholar
- Anuj KM, Mohd K, Sanjaya KP (2010) Evaluation of analgesic activity of methanolic extract of Trapa natans l.var. Bispinosa roxb. roots. J Curr Pharmaceut Res 1:8–11Google Scholar
- Chopra RX, Chopra IC, Handa KL, Kapur LD (1958a) Indigenous drugs of India, 2nd edn. Academic Publisher, CalcuttaGoogle Scholar
- Chopra RN, Chopra IC, Handa KL, Kapur LD (1958b) Indigenous drugs of India, 2nd edn. Academic Publisher, Calcutta, p 674Google Scholar
- Ezeonu CS, Ejikeme CM (2016) Qualitative and quantitative determination of phytochemical contents of indigenous Nigerian softwoods. New J Sci Article ID 5601327: 1–6Google Scholar
- Geetha TS, Geetha N (2014) Quantitative analysis of primary and secondary metabolites of Cymbopogan citratus (DC) stapf. leaves from Kodaikanal hills, Tamil nadu. Int J PharmTech Res 6(2):521–529Google Scholar
- Harborne JB (1973) Phytochemical methods: a guide to modern techniques of plant analysis. Chapman and Hall Publishers, London, pp 309–316Google Scholar
- Headley PM, Shaughnessy CT (1985) Evidence for opiate and dopamine interaction in striatum. Br J Pharmacol 86:700Google Scholar
- ICH (2005) Q2A. Validation of analytical procedures: text and methodology. International conference on harmonization, GenevaGoogle Scholar
- Kirtikar KR, Basu BD (1975) Indian medicinal plants. International Book Distributers, Dehradun, pp 2327–2328Google Scholar
- Nadkarni KM, Nadkarni AK, Chopra RN (1996) India Materia Medica. Popular Prakashan, Bombay, pp 548–550Google Scholar
- OECD Guidance document on acute oral toxicity 420, NO. 24. Environmental health and safety monograph series on testing assessment (2000) 21: 155–159Google Scholar
- Piao G, Yuan H, Ma Q, Ye L (2016) The traditional medicine and modern medicine from natural products. Molecules 21(559):1–18Google Scholar
- Raj SJ, Joseph B (2010) Phytopharmacological and phytochemical properties of three Ficus species—an overview. Int J Pharm Biol Sci 1(4):246–253Google Scholar
- Rajani GP, Gupta D, Sowjanya K, Sahithi B (2011) Screening of antipyretic activity of aerial parts of Nelumbo nucifera Gaertn. in yeast induced pyrexia. Pharmacologyonline 1:1120–1124Google Scholar
- Shakya AK (2016) Medicinal plants: future source of new drugs. Int J Herb Med 4(4):59–64Google Scholar
- Sharma P (2001) DravyaGuna-Vigyan (Aubhid Ausadh –Dravya). Chaukhambha Bhartiya Academy, Varanasi, pp 80–83Google Scholar
- Ekanayake DT. Sri Lanka Forest (1980) 14(1,4):145–152Google Scholar
- Tejavathi DH, Jayashree DR (2013) Phytochemical screening of selected medicinal herbs inoculated with Arbuscular mycorrhizal fungi. Int J Pharm Biol All Sci 2(11):2090–2106Google Scholar
- Trease GE, Evans WC (2002) Pharmacognosy, 15th edn. Saunders Publishers, London, pp 393–399Google Scholar
- Vogel GH, Vogel WH (1997) Analgesic, anti-inflammatory and antipyretic activity. In: Drug discovery and evaluation. Pharmacol ass. Springer, Berlin, pp 360–418Google Scholar
- Wigdor S, Wilcox GL (1987) Central and systemic morphine-induced antinociception in mice: of contribution descending serotonergic and noradrenergic pathways. J Pharmacol Exp Ther 242:90Google Scholar
- Woolfe G, MacDonald AD (1944) The evaluation of the analgesic action of pethidine hydrochloride (DEMEROL). J Pharmacol Exp Ther 80:300–307Google Scholar