Evaluation of antitumor activity of Urena lobata against Ehrlich ascites carcinoma treated mice
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The search for cancer therapy with natural products continues to be a global effort. Many natural products have been tested globally against various cancer cell lines. Urena lobata Linn. (U. lobata) has been used as an ingredient in various formulations of traditional medicines for the treatment of cancers. The present study was undertaken to investigate the antitumor activity of the methanol (MEUL) and aqueous (AEUL) extracts of the U. lobata whole herb in Swiss Albino mice against Ehrlich ascites carcinoma (EAC) cell line. Both extracts were administered orally (200 mg/kg and 400 mg/kg) for 13 consecutive days. After 24 h of the last dose, the animals were scarified. The antitumor effect of the MEUL and AEUL was determined by evaluating the median survival time (days), %ILS, tumor volume, viable tumor cell count, non-viable tumor cell count and tumor weight (g) and hematological parameters of EAC-bearing mice. Both MEUL and AEUL showed significant decrease in tumor volume, the viable cell count, tumor weight, elevated mean survival time, %ILS and non-viable cell count. Hematological profile such as RBC, WBC, and haemoglobin count was reverted to normal level in treated mice. In conclusion, MEUL (400 mg/kg) showed prominent antitumor activity against EAC treated mice.
KeywordsUrena lobata Caesar weed Congo jute Ehrlich ascites carcinoma Antitumor 5-Fluorouracil
The authors wish to acknowledge M/s. Green Chem Herbal Extracts & Formulations, Bangalore, India for extending their facility for preparation of our plant extracts.
Compliance with ethical standards
The study protocol was approved by the Institutional Animal Ethics Committee (Ref No: IAEC/08/06/2012). All the animals were cared and experimented in accordance with the highest scientific, humane, and ethical principles described in Guide for the Care and Use of Laboratory Animals (U.S. 1996) and Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA 2003).
Conflict of interest
This manuscript described has not been published before; not under consideration for publication anywhere else; and has been approved by all co-authors.
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