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Anticonvulsant and neuroprotective effects of methanolic extract of Cinnamomum camphora leaves in rat brain

  • Talha Jawaid
  • Mehnaz Kamal
  • Richa Singh
  • Deepa Shukla
  • Vidya Devanathadesikan
  • Mukty Sinha
Research Article
  • 16 Downloads

Abstract

The aim of this study was to investigate the anticonvulsant and neuroprotective effects of methanolic extract of Cinnamomum camphora leaves (MECC) in albino wistar rats against maximal electroshock seizure (MES) and seizures induced by pentylenetetrazol (PTZ) models. Acetylcholinesterase (AChE) activity and oxidative stress parameters like malondialdehyde (MDA) and reduced glutathione (GSH) were estimated in the brains after completion of the anticonvulsant studies. MECC (50 and 100 mg/kg b.w., p.o.) exhibited anticonvulsant activity as indicated by significant (P < 0.05, P < 0.01) reduction in the duration of hind limb tonic extensor phase in MES induced seizure model and significantly (P < 0.05, P < 0.01) increased the time of onset of clonic convulsion, decreased the duration of seizures, increased the % protection and decreased the percent mortality in a dose dependent manner. MECC (50 and 100 mg/kg b.w., p.o.) exhibited neuroprotective activity as indicated by significant reduction in MDA levels, AChE activity and increased GSH level also in a dose dependent manner. These results indicate that MECC may exert anticonvulsant and neuroprotective effects which may be attributed to the increase in the level of GABA, inhibition of AChE and inflammation and antioxidant activity in the brain.

Keywords

Anticonvulsant Neuroprotective MES PTZ AChE Oxidative stress parameters 

Notes

Acknowledgements

The authors are thankful to Hygia Institute of Pharmaceutical Education and Research, Lucknow, India for providing necessary facilities to carry out this research. Authors would also like to thank National Botanical Research Institute, Lucknow, India for plant authentication and Central Drug Research Institute, Lucknow, India for providing animals.

Compliance with ethical standards

Ethical statement

All animal experiments were approved by Animal Services Division of Central Drug Research Institute, Lucknow, India (1087/07/CPCSEA).

Conflict of interest

The authors report no declaration of interest.

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Copyright information

© Institute of Korean Medicine, Kyung Hee University and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Talha Jawaid
    • 1
  • Mehnaz Kamal
    • 2
  • Richa Singh
    • 3
  • Deepa Shukla
    • 4
  • Vidya Devanathadesikan
    • 5
  • Mukty Sinha
    • 6
  1. 1.Department of Pharmacology, College of MedicineDar Al Uloom UniversityRiyadhKingdom of Saudi Arabia
  2. 2.Department of Pharmaceutical Chemistry, College of PharmacyPrince Sattam Bin Abdulaziz UniversityAl-KharjKingdom of Saudi Arabia
  3. 3.Department of PharmacologyHygia Institute of Pharmaceutical Education and ResearchLucknowIndia
  4. 4.Department of PharmacologyRaj Kumar Goel Institute of TechnologyGhaziabadIndia
  5. 5.Department of Pharmacology, College of PharmacyPrince Sattam Bin Abdulaziz UniversityAl-KharjKingdom of Saudi Arabia
  6. 6.Department of Medical DevicesNIPER-AhmedabadGandhinagarIndia

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