Oriental Pharmacy and Experimental Medicine

, Volume 18, Issue 2, pp 159–165 | Cite as

Bioactivities of leaf and root extract of Ceriscoids turgida (Roxb.)

  • Md. Nazmul Hasan ZilaniEmail author
  • Nazneen Ahmeda Sultana
  • Monjurul Kader Bakshi
  • Israt Jahan Shampa
  • Samia Jaman Sumi
  • Ohidul Islam
Research Article


In traditional medicine leaf and root of Ceriscoides turgida (Roxb.) are commonly used to treat indigestion in child, leucorrhoea, dog bite, snake bite, scorpion string, epilepsy, stomachache and a number of ailments. The present study was carried out to investigate antioxidant, analgesic, antibacterial and cytotoxic activities of methanol extract of leaves and roots of C. turgida. Total flavonoids content was spectrophotometrically determined using aluminum chloride colorimetric assay while total phenolic and tannin content by Folin Chiocalteu’s reagent. Antioxidant activity was determined by DPPH free radical scavenging and reducing power assay. Analgesic activity was assessed by acetic acid induced writhing method. Antibacterial activity was evaluated by disc diffusion assay while brine shrimp lethality bioassay was carried out to verify the cytotoxic potential. Root extract contains greater amount of total phenolic (79.99 mg GAE/g dry extract) and flavonids content (35.27 mg QE/g dry extract) in comparison to leaf extract (30.75 mg GAE/g and 25.93 mg QE/g dry extract respectively). Root extracts showed DPPH scavenging (157.45 μg/mL) that was comparable to standard ascorbic acid (14.15 μg/mL). Reducing power of root extract was prominent than leaf extract in comparison to ascorbic acid. Both extract showed significant (P < 0.01) analgesic activity. In disc diffusion assay leaf extract showed greater antibacterial activity than root extract. Furthermore, extracts showed moderate cytotoxic effect. The present study demonstrates that methanol extract of C. turgida leaf and root has significant antioxidant and analgesic effect. The extracts also showed moderate antibacterial and cytotoxic activities.


Total phenolic content Analgesic activity Antibacterial activity Cytotoxic activity Ceriscoides turgida 



The authors are grateful to the Department of Pharmacy, Bangladesh University, Dhaka, Bangladesh for providing test microorganisms.

Compliance with Ethical Standards

Ethical Statement

Our study was approved by a research Ethics Committee for Animal House of Department of Pharmacy, Faculty of Science, Engineering and Technology, Bangladesh University, Dhaka-1207, Bangladesh. Two members ethics committee consists of Dr. Md. Anwer Hossain, Associate Professor and chairman of the committee, A K Azad, Assistant Professor; Department of Pharmacy, Faculty of Science, Engineering and Technology, Bangladesh University, Dhaka-1207, Bangladesh.

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© Institute of Korean Medicine, Kyung Hee University and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Md. Nazmul Hasan Zilani
    • 1
    • 2
    Email author
  • Nazneen Ahmeda Sultana
    • 1
    • 2
  • Monjurul Kader Bakshi
    • 2
  • Israt Jahan Shampa
    • 2
  • Samia Jaman Sumi
    • 2
  • Ohidul Islam
    • 2
  1. 1.Department of Pharmacy, Faculty of Allied Health SciencesDaffodil International UniversityDhakaBangladesh
  2. 2.Department of Pharmacy, Faculty of Science, Engineering and TechnologyBangladesh UniversityDhakaBangladesh

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