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A randomized, double-blind study comparing multiple doses of Channa striatus supplementation for knee osteoarthritis

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Abstract

Knee osteoarthritis (OA) is the leading cause of chronic disability at older age. Channa striatus (CS) is a freshwater fish that is traditionally valued for its medicinal properties in promoting wound healing and reducing post-operative pain. This study evaluate the efficacy of different doses of oral Channa striatus extract on primary knee osteoarthritis patients. A randomized, double-blind, placebo-controlled 3-arm trial was conducted comparing oral CS extract 1000 mg/day or 500 mg/day and placebo among knee OA patients for a 6-month intervention period. The main outcome measures were Western Ontario and McMaster University Osteoarthritis Index (WOMAC), analgesic scores and serum cartilage oligomeric matric protein (COMP). Laboratory-based blood tests were used as safety measures. A total of 120 patients were randomized, and 112 patients were included in the intention-to-treat analysis. Significant reductions in WOMAC stiffness and function scores were achieved at month 6 in CS 1000 mg/day and CS 500 mg/day compared to placebo groups (p < 0.05). No significant differences were found between the groups in terms of analgesic scores, serum COMP and biochemical parameters. No serious adverse events were reported in the study. In conclusion, both doses of CS showed similar efficacy and were more effective than the placebo in treating the symptoms of knee OA.

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References

  • Ab Wahab SZ et al (2015) The effect of Channa striatus (Haruan) extract on pain and wound healing of post-lower segment caesarean section women. Evid. Based Complement Alternat Med eCAM 849647:2015. https://doi.org/10.1155/2015/849647

    Google Scholar 

  • Abadie E et al (2004) Recommendations for the use of new methods to assess the efficacy of disease-modifying drugs in the treatment of osteoarthritis osteoarthritis and cartilage / OARS. Osteoarthr Res Soc 12:263–268. https://doi.org/10.1016/j.joca.2004.01.006

    Article  Google Scholar 

  • Abu Bakar MR et al (2015) Randomized controlled trial on the effect of Channa striatus extract on measurement of the uterus, Pulsatility index, resistive index of uterine artery and superficial skin wound artery in post lower segment caesarean section women. PLoS One 10:e0133514. https://doi.org/10.1371/journal.pone.0133514

    Article  PubMed Central  PubMed  Google Scholar 

  • Al-Saffar FJ, Ganabadi S, Fakurazi S (2011a) Response of Channa striatus extract against monosodium Iodoacetate induced osteoarthritis in rats. J Anim Vet Adv 10:460–469

    Article  Google Scholar 

  • Al-Saffar FJ, Ganabadi S, Fakurazi S, Yaakub S (2011b) Zerumbone significantly improved immunoreactivity in the synovium compared to Channa striatus extract in monosodium iodoacetate (MIA)- induced knee ostearthritis in rat. J Med Plant Res 5:1701–1710

    CAS  Google Scholar 

  • Andersson ML, Thorstensson CA, Roos EM, Petersson IF, Heinegård D, Saxne T (2006) Serum levels of cartilage oligomeric matrix protein (COMP) increase temporarily after physical exercise in patients with knee osteoarthritis. BMC Musculoskelet Disord 7:98

    Article  PubMed Central  PubMed  Google Scholar 

  • Altman R, Asch E, Bloch D, Bole G, Borenstein D, Brandt K, Christy W, Cooke TD, Greenwald R, Hochberg M. et al. (1986) Development of criteria for the classification of osteoarthritis. Classification of osteoarthritis of the knee. Diagnostic and Therapeutic Criteria Committee of the American Rheumatism Association. Arthritis Rheum 29(8):1039–1049

  • Ausiello JC, Stafford RS (2002) Trends in medication use for osteoarthritis treatment. J Rheumatol 29:999–1005

    PubMed  Google Scholar 

  • Aydın E, Turan Y (2016) Biochemical markers for osteoarthritis: is there any promising candidate? Meandros. Med Dent J 17:27–34

    Google Scholar 

  • Baie SH, Sheikh K (2000a) The wound healing properties of Channa Striatus-cetrimide cream-wound contraction and glycosaminoglycan measurement. J Ethnopharmacol 73:15–30

    Article  CAS  PubMed  Google Scholar 

  • Baie SH, Sheikh K (2000b) The wound healing properties of Channa Striatus-cetrimide cream—tensile strength measurement. J Ethnopharmacol 71:93–100

    Article  CAS  PubMed  Google Scholar 

  • Bellamy N (2014) Womac Osteoarthritis Index: A user’s guide XI. The University of Queensland, Brisbane

  • Bertrand J, Cromme C, Ummlauf D, Frank S, Pap T (2010) Molecular mechanism of cartilage remodelling in osteoarthritis. Int J Biochem Cell Biol 42(10):1594–1601

  • Cheng DS, Visco CJ (2012) Pharmaceutical therapy for osteoarthritis. PMR 4:S82–S88. https://doi.org/10.1016/j.pmrj.2012.02.009

    Article  Google Scholar 

  • Chua S, Messier SP, Legault C, Lenz ME, Thonar E-M, Loeser RF (2008) Effect of an exercise and dietary intervention on serum biomarkers in overweight and obese adults with osteoarthritis of the knee. Osteoarthr Cartil 16:1047–1053

    Article  PubMed Central  PubMed  Google Scholar 

  • Clark AG, Jordan JM, Vilim V, Renner JB, Dragomir AD, Luta G, Kraus VB (1999) Serum cartilage oligomeric matrix protein reflects osteoarthritis presence and severity. Arthritis Rheum 42:2356e2364

    Article  Google Scholar 

  • Dahlan-Daud CK, Jais AM, Ahmad Z, Akim AM, Adam A (2010) Amino and fatty acid compositions in Haruan traditional extract (HTE). Bol Latinoam Caribe Plant Med Aromat 9:414–429

    CAS  Google Scholar 

  • Das BR, Roy A, Khan FR (2015) Cartilage oligomeric matrix protein in monitoring and prognostication of osteoarthritis and its utility in drug development. Perspect Clin Res 6:4

    Article  PubMed Central  PubMed  Google Scholar 

  • Dupont WD, Plummer WD (1997) PS power and sample size program available for free on the internet. Control Clin Trials 18:274

    Article  Google Scholar 

  • Ganabadi S (2009) Channa Striatus extract supplementation significantly increased protein gene product 9.5-immunoreactive nerve fibres compared to zingiber officinale extract in collagenase induced osteaoarthritis. Osteoarthr Cartil 17:S281–S282. https://doi.org/10.1016/S1063-4584(09)60546-X

    Article  Google Scholar 

  • Goldring MB, Otero M (2011) Inflammation in osteoarthritis. Curr Opin Rheumatol 23:471–478. https://doi.org/10.1097/BOR.0b013e328349c2b1

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Hammad YH, Magid HR, Sobhy MM (2015) Clinical and biochemical study of the comparative efficacy of topical versus oral glucosamine/chondroitin sulfate on osteoarthritis of the knee. Egypt Rheumatologist 37:85–91

    Article  Google Scholar 

  • Hoch JM, Mattacola CG, McKeon JM, Howard J, Lattermann C (2011) Serum cartilage oligomeric matrix protein (sCOMP) is elevated in patients with knee osteoarthritis: a systematic review and meta-analysis. Osteoarthr Cartil 19:1396–1404

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Huang SM et al (2001) Identification of a new class of molecules, the arachidonyl amino acids, and characterization of one member that inhibits pain. J Biol Chem 276:42639–42644

    Article  CAS  PubMed  Google Scholar 

  • Isa IIM, Bakar SA, Tohid SFM, Jais AMM (2016) Channa Striatus cream down-regulates tumour necrosis factor (TNF)-alpha gene expression and alleviates chronic-like dermatitis in mouse model. J Ethnopharmacol 194:469–474

    Article  Google Scholar 

  • James MJ, Gibson RA, Cleland LG (2000) Dietary polyunsaturated fatty acids and inflammatory mediator production. Am J Clin Nutr 71:343s–348s

    CAS  PubMed  Google Scholar 

  • Jordan JM et al (2003) Ethnic and sex differences in serum levels of cartilage oligomeric matrix protein: the Johnston County osteoarthritis project. Arthritis Rheumatism 48:675–681

    Article  CAS  PubMed  Google Scholar 

  • Kadir AA, Wahab SZA, Zulkifli MM, Nor NM, Haron J (2014) The therapeutic effect of the oral Channa striatus extract on primary knee osteoarthritis patients. Agro Food Ind Hi Tech 25:44–48

    Google Scholar 

  • Laila L, Febriyenti F, Salhimi SM, Baie S (2011) Wound healing effect of Haruan ( Channa Striatus) spray. Int Wound J 8:484–491. https://doi.org/10.1111/j.1742-481X.2011.00820.x

    Article  PubMed  Google Scholar 

  • Lohmander LS, Saxne T, Heinegård D (1994) Release of cartilage oligomeric matrix protein (COMP) into joint fluid after knee injury and in osteoarthritis. Ann Rheum Dis 53:8–13

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Mat Jais AM (2007) Pharmacognosy and pharmacology of Haruan (Channa striatus), a medicinal fish with wound healing properties, vol 6. Derechos de Publication, Santiago

    Google Scholar 

  • Mat Jais AM, Dambisya YM, Lee T-L (1997) Antinociceptive activity of Channa Striatus (haruan) extracts in mice. J Ethnopharmacol 57:125–130. https://doi.org/10.1016/S0378-8741(97)00057-3

    Article  CAS  PubMed  Google Scholar 

  • Mat Jais AM, Zakaria ZA, Luo A, Song YX (2008) Antifungal activity of Channa striatus (Haruan) crude extracts. Int J Trop Med 3:43–48

    Google Scholar 

  • McConnell S, Kolopack P, Davis AM (2001) The western Ontario and McMaster universities osteoarthritis index (WOMAC): a review of its utility and measurement properties. Arthritis Care Res 45:453–461

    Article  CAS  Google Scholar 

  • Michelle NYT, Shanti G, Loqman MY (2004) Effect of orally administered Channa Striatus extract against experimentally-induced osteoarthritis in rabbits. Int J Appl Res Vet 2:171–175

    Google Scholar 

  • Mohamad Isa II, Abu Bakar S, Md Tohid SF, Mat Jais AM (2016) Channa Striatus cream down-regulates tumour necrosis factor (TNF)-alpha gene expression and alleviates chronic-like dermatitis in mouse model. J Ethnopharmacol 194:469–474. https://doi.org/10.1016/j.jep.2016.10.033

    Article  PubMed  Google Scholar 

  • Mohamed N, Hamdan MR, Salleh SNM, bin Bai S (2014) Effect of freeze drying and spray drying processes to amino acids and fatty acids contents in Haruan (Channa Striatus) extract. Int J Drug Deliv 6:301–304

    Google Scholar 

  • Mohd SMA, Abdul Manan MJ (2012) Therapeutic potential of the haruan (Channa Striatus): from food to medicinal uses. Malays J Nutr 18:125–136

    PubMed  Google Scholar 

  • Mohtar N, Mohamed N, Hamdan MR, Salleh SNM, bin Bai S (2014) Comparison of freeze drying and spray drying methods of Haruan extract. Int J Drug Deliv 6:286–291

    Google Scholar 

  • Mullen A, Loscher CE, Roche HM (2010) Anti-inflammatory effects of EPA and DHA are dependent upon time and dose-response elements associated with LPS stimulation in THP-1-derived macrophages. J Nutr Biochem 21:444–450. https://doi.org/10.1016/j.jnutbio.2009.02.008

    Article  CAS  PubMed  Google Scholar 

  • Paliliewu N, Datau EA, Matheos JC, Surachmanto EE (2013) Channa Striatus capsules induces cytokine conversion in pulmonary tuberculosis patients. J Exp Integr Med 3:237–242

    Article  Google Scholar 

  • Pascarelli NA, Cheleschi S, Bacaro G, Guidelli GM, Galeazzi M, Fioravanti A (2015) Effect of mud-bath therapy on serum biomarkers in patients with knee osteoarthritis: results from a randomized controlled trial. Isr Med Assoc J 18:232–237

    Google Scholar 

  • Pasha M, Huin RA, Hassan S (2015) The influence of oral and topical Channa Striatus on laparotomy wound healing in malnourished Wistar rats. Int J Pharm Sci Invent 4:37–41

    CAS  Google Scholar 

  • Petersen S et al (2010) Glucosamine but not ibuprofen alters cartilage turnover in osteoarthritis patients in response to physical training osteoarthritis and cartilage / OARS. Osteoarthr Res Soc 18:34–40

    Article  CAS  Google Scholar 

  • Shin J-W, Seol I-C, Son C-G (2010) Interpretation of animal dose and human equivalent dose for drug development. J Koresn Orient Med 31:1–7

    Google Scholar 

  • Van Spil W, Welsing P, Bierma-Zeinstra S, Bijlsma J, Roorda L, Cats H, Lafeber F (2015) The ability of systemic biochemical markers to reflect presence, incidence, and progression of early-stage radiographic knee and hip osteoarthritis: data from CHECK. Osteoarthr Cartil 23:1388–1397

    Article  PubMed  Google Scholar 

  • Witte MB, Barbul A (2002) Role of nitric oxide in wound repair. Am J Surg 183:406–412

    Article  CAS  PubMed  Google Scholar 

  • Zakaria ZA, Mat Jais AM, Goh YM, Sulaiman MR, Somchit MN (2007) Amino acid and fatty acid composition of an aqueous extract of Channa Striatus (Haruan) that exhibits antinociceptive activity. Clin Exp Pharmacol Physiol 34:198–204

    Article  CAS  PubMed  Google Scholar 

  • Zhang W et al (2008) OARSI recommendations for the management of hip and knee osteoarthritis, part II: OARSI evidencebased, expert consensus guidelines osteoarthritis and cartilage / OARS. Osteoarthr Res Soc 16:137–162

    Article  CAS  Google Scholar 

  • Zhang W et al (2010) OARSI recommendations for the management of hip and knee osteoarthritis: part III: changes in evidence following systematic cumulative update of research published through January 2009 osteoarthritis and cartilage / OARS. Osteoarthr Res Soc 18:476–499

    Article  CAS  Google Scholar 

  • Zuraini A et al (2005) Fatty acid and amino acid composition of three local Malaysian Channa spp. fish. Food Chem 97:674–678

    Article  Google Scholar 

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Acknowledgements

This work was supported by the by Universiti Sains Malaysia Research University grant (grant number: 304/PPSP/661313181).The funder has no role in this study other than to provide the funding and all authors are independent from the funder. We would like to acknowledge the late Prof. Saringat Bai @ Baie and Assoc. Prof Zuraini Ahmad for their contributions to the study.

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Author notes

  1. Abdul Manan Mat Jais

    Present address: Abmanan Biomedical Sdn Bhd (ABSB), A-G-1, Univ 360 Place, Jalan Raya 2, Taman Serdang Raya, 43300, Seri Serdang, Selangor, Malaysia

Authors and Affiliations

  1. Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Universiti Putra Malaysia, Serdang, Selangor, Malaysia

    Abdul Kadir Azidah

  2. Department of Family Medicine, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia

    Abdul Kadir Azidah, Azlina Ishak & Norhayati Mohd. Noor

  3. Department of Veterinary Pre Clinical Sciences, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor Darul Ehsan, Malaysia

    Abdul Kadir Arifah

  4. Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    A. H. Roslida & Abdul Manan Mat Jais

  5. Department of Chemical Pathology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia

    Julia Omar

  6. Department of Orthopedic, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia

    Abdul Nawfar Sadagatullah

  7. Department of Radiology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia

    Ahmad Tarmizi Musa

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  1. Abdul Kadir Azidah
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Corresponding author

Correspondence to Abdul Kadir Arifah.

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Ethical Statement

The research proposal was approved by the Human Research Ethics Committee, Universiti Sains Malaysia (Reference number: USM / JEPeM/ [272.2(10)]. This trial is registered in the Thai Clinical Trials Registry (TCTR). The registration number is TCTR20170513004. The registry website http://www.clinicaltrials.in.th/index.php.

Conflict of Interest

All authors declared no conflict of interest.

Informed consent

Written informed consent was obtained from all individual participants included in the study.

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Azidah, A., Arifah, A., Roslida, A.H. et al. A randomized, double-blind study comparing multiple doses of Channa striatus supplementation for knee osteoarthritis. Orient Pharm Exp Med 17, 345–354 (2017). https://doi.org/10.1007/s13596-017-0293-7

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