Lasianthera Africana leaves inhibits α-amylase α-glucosidase, angiotensin-I converting enzyme activities and Fe2+-induced oxidative damage in pancreas and kidney homogenates
- 110 Downloads
Lasianthera africana is a dark green leafy vegetable commonly consumed as food and used for treatment of several human ailments in Nigeria most especially south-east. This study investigated the phenolic composition and effects of methanol extract from L. africana leaves on enzymes linked to type-2 diabetes (α-amylase and α-glucosidase) and hypertension [angiotensin-1-converting enzymes (ACE)] as well as Fe2+-induced lipid peroxidation in rat’s pancreas and kidney homogenates were evaluated. Phenolic contents and constituents were also determined using colorimetric methods and high performance liquid chromatography-diode array detector (HPLC-DAD) respectively. Our findings revealed that the extract inhibited α-amylase, α-glucosidase and ACE activities with IC50 values of 0.192 and 0.21 mg/mL, and 57.06 μg/mL respectively. The extract also inhibited Fe2+-induced lipid peroxidation in rats’ pancreas and kidney homogenates. Our findings revealed that the extract is rich in phenolic acids such as caffeic acid (59.97 mg/g), chlorogenic acid (58.23 mg/g) and ellagic acid (31.16 mg/g) as well as flavonoids such as quercetin (42.98 mg/g). These results give a clue to the potential use of L. africana leaves as an alternative agent for the control of glucose absorption and lowering of blood pressure in diabetic patients with hypertension.
KeywordsLasianthera africana α-amylase α-glucosidase Angiotensin-1-converting enzyme antioxidant
Authors acknowledge every member of Functional Food and Nutracetical Laboratory Unit of Biochemistry departement, Federal Univervisty of Technology, Akure, where this work was carried out.
SAS, GO and SIO designed the study, participated in laboratory works, read the first draft and approved the final draft. SIO, TAO and AAA carried out sample preparation, laboratory experiment, collection and interpretation of data. AAB carried out the HPLC-DAD analysis.
Compliance with ethical standards
This research was not funded by any grant received by any of the authors.
Conflict of Interest
The authors declare no conflict of interest.
Ethical approval was obtained from the Animal ethical committee of the School of Science Federal University of Technology Akure with Approval No FUTA/SOS/1399. Handling of the animals was in accordance with the Guide for Care and Use of Laboratory Animals prepared by the National Academy of Science which was published by the National Institute of Health (USA)
- Ademosun AO, Oboh G, Adewuni TM, Akinyemi AJ, Olasehinde TA (2013) Antioxidative properties and inhibition of key enzymes linked to type-2 diabetes by snake tomato (Tricosanthes cucumerina) and two tomato (Lycopersicon esculentum) varieties. Afr J Pharm Pharmacol 7(33):2358–2365CrossRefGoogle Scholar
- Alobi NO, Ikpeme EM, Okoi AI, Etim KD, Eja ME (2012) Phytochemical and nutritional profiles of Lasianthera africana, Heinsia crinata and Gongronema latifolium. New York Sci J 5(3):45–48Google Scholar
- Andy IE, Eja ME, Mboto CI (2008) An evaluation of the antimicrobial potency of L. africana (Beauv) and Heinsia crinata (G. Taylor) on E. coli, Salmonella typhi, Staphylococcus aureus and Candida albicans. Malaysian J Microbiol 4(1):25–29Google Scholar
- Asmat U, Abad K, Ismail K (2015) Diabetes mellitus and oxidative stress—A concise review. doi: 10.1016/j.jsps.2015.03.013
- Ayepola OR, Brooks NL, Oguntibeju OO (2014) Oxidative stress and diabetic complications: the role of antioxidant vitamins and flavonoids. doi: 10.5772/57282
- Bakris GL, Williams M, Dworkin L, Elliott WJ, Epstein M, Toto R, Tuttle K, Douglas J, Hsueh W, Sowers J (2000) Preserving renal function in adults with hypertension and diabetes: a consensus approach. National Kidney Foundation hypertension and diabetes executive committees working group. Am L Kidney Dis 36:646–661CrossRefGoogle Scholar
- Cerf ME (2013) Beta cell dysfunction and insulin resistance. Front Endocrinol (Lausanne) 4:37Google Scholar
- Cheung BM, Li C (2012) Diabetes and hypertension: is there a common metabolic pathway?. Curr Atherosclerosis Reports 14(2): 160–166Google Scholar
- Chugh SS, Reinier K, Teodorescu C, Evanado A. Kehr E. Al Samara M. Mariani R. Gunson K, Jui J (2008) Epidemiology of sudden cardiac death: clinical and research implications. Progress in Cardiovascular Diseases 51(3): 213–228Google Scholar
- Ebana RUB, Essien AI, Ekpa OD (1995) Nutritional and potential medicinal values of the leaves of Lasianthera africana (Beauv). Global J Pure Appl Sci 1(1):1–8Google Scholar
- Ghasemzadeh A, Ghasemzadeh N (2011) Flavonoids and phenolic acids: role and biochemical activity in plants and human. J Med Plants Res 5(31):6697–6703Google Scholar
- Itah AY (1997) Bactericidal and bacteriostatic effect of edible leafy vegetable extract on growth of canned food borne bacteria. Trans Nig Soc Bio Conserv 6:103–111Google Scholar
- Kang WY, Wang JM, Zhang L (2010) α-glucosidase inhibitors from forsythia suspense (Thunb) Vahl, China. J Chin Mater Med 35:1156–1159Google Scholar
- Oboh G, Ademiluyi AO, Ademosun AO, Olasehinde TA, Oyeleye SI, Boligon AA, Athayde ML (2015c) Phenolic extract from moringa oleifera leaves inhibits key enzymes linked to erectile dysfunction and oxidative stress in rats' penile tissues. Biochem Res Int 2015:175950. doi: 10.1155/2015/175950 CrossRefPubMedPubMedCentralGoogle Scholar
- Okokon JE, Antia BS, Essiet GA (2007) Evaluation of in vivo antiplasmodial activity of ethanolic leaf extract of Lasianthera africana. Res J Pharmacol 1(2):30–33Google Scholar
- Padilla-Camberos E, Lazcano-Díaz E, Flores-Fernandez JM, Owolabi MS, Allen K, Villanueva-Rodríguez S (2014) Evaluation of the inhibition of carbohydrate hydrolyzing enzymes, the antioxidant activity, and the polyphenolic content of Citrus limetta peel extract. Sci World J 2014:121760CrossRefGoogle Scholar
- Shodehinde SA, Adefegha SA, Oboh G, Oyeleye SI, Olasehinde TA, Nwanna EE, Adedayo BC, Boligon AA (2016) Phenolic composition and evaluation of methanol and aqueous extracts of bitter gourd (Momordica charantia L) leaves on angiotensin-I-converting enzyme and some pro-oxidant-induced lipid peroxidation in vitro. J Evid Based Comp Alt Med 21:NP67CrossRefGoogle Scholar
- Singleton VL, Orthofer R, Lamuela RRM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin– Ciocalteu reagent. In: Packer L (ed) Methods in enzymology, Oxidants and antioxidants, vol 299. Part A. San Diego, Academic Press, pp 152–178Google Scholar
- Tiwari AK, Rao JM (2002) Diabetes mellitus and multiple therapeutic approaches of phytochemicals: present status and future prospects. Curr Sci 83(1):30–38Google Scholar
- Usmani S (2013) Screening for antioxidant and free radical scavenging potential of extracts of leaves and flowers of Calotropis gigantea. Asian J Pharm Clin Res 6:97–100Google Scholar
- Worthington V (1993) Alpha amylase. In: Worthington enzyme manual. Worthington Biochemical Corp, Freehold, pp 36–41Google Scholar