Immunomodulatory properties and biomarkers characterization of novel Withania somnifera based formulation supplemented with minerals in Sprague Dawley rats
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Herbomineral formulations have been accepted world-wide due to its significant therapeutic outcomes. The study aimed to evaluate the immunomodulatory potential of novel herbomineral formulation (TEBEH) on Sprague Dawley rats using cyclophosphamide-induced immunosuppression. Major immune related parameters were monitored such haemagglutination assay, delayed type hypersensitivity reaction, cellular immune response (CD4+ and CD8+), IgG and IgM, hematological analysis, antioxidant profile, and estimation of major lipid biomarkers. The study results suggest herbomineral formulation significantly (p ≤ 0.05) improved the concentration of primary and secondary titre by 41.18 and 68%. However, the rat paw edema at 24 h showed increased edema by 77.78% (p ≤ 0.01). Herbomineral formulation exhibited increase in the CD4+ and CD8+ counts in animals by 28.03 and 60.70%, respectively. In addition, the concentrations of IgM and IgG were improved by 14.29 and 8.26% respectively. Besides, other supportive parameter such as hematology and antioxidant profile was significantly (p ≤ 0.001) improved, while free radical, lipid peroxidation was decreased. Moreover, the major lipid biomarkers were improved such as decreased triglycerides, LDL and VLDL by 15.88, 3.65, and 16.01%, respectively. Overall, the promising results of this study indicated that the herbomineral formulation, TEBEH can be employed to improve the cellular and humoral immunity along with improved hematological, antioxidant, and lipid profile.
KeywordsImmunopotentiation Herbomineral product Cellular and humoral immunity Hematological parameters Lipid profile Antioxidant profile
The authors extend their sincere thanks and grateful to Dabur Research Foundation, Ghaziabad, India for providing the facilities and support that enabled the successful completion of the work. Authors gratefully acknowledged to GVK Biosciences Pvt. Ltd., Hyderabad, India for their support in chemical characterization of ashwagandha root extract.
Compliance with ethical standards
All the animals used in this experiment were approved by Animal Ethics Committee approved protocol (IAEC/36/394) and the husbandry conditions maintained as per CPCSEA recommendations.
Conflicts of Interest
The authors declared no conflicts of interest in this work.
- Conforti F, Sosa S, Marrelli M, Menichini F, Statti GA, Uzunov D, Tubarob A, Menichinia F (2009) The protective ability of Mediterranean dietary plants against the oxidative damage: The role of radical oxygen species in inflammation and the polyphenol, flavonoid and sterol contents. Food Chem 112:587–594CrossRefGoogle Scholar
- Cooper EL (2004) Complementary and alternative medicine when rigorous can be science. Evid. Based Complement. Alternat Med 1:1–4Google Scholar
- Dale MM, Forman JC (1989) Textbook of immunopharmacology, 2nd edn. Blackwell and Scientific Publication, OxfordGoogle Scholar
- Dhawan BN, Srimal RC (1980) India: United Nations Industrial Development Organization and International Center for Science and High Technology; Acute toxicity and gross effects. In: Laboratory Manual for Pharmacological Evaluation of Natural Products; pp. 17–20Google Scholar
- Feldman BF, Zinkl JG, Jain VC (2000) Laboratory techniques for avian hematology in Schalm’s Veterinary Hematology, 5th edn. Lippincott Williams & Wilkins, TorontoGoogle Scholar
- Girdhari L, Rana A (2007) Withania somnifera (Ashwagandha): A review. Pharmacog Rev 1:129–136Google Scholar
- Gunasekara P, Hettiarachchi M, Liyanage C, Lekamwasam S (2011) Effects of zinc and multimineral vitamin supplementation on glycemic and lipid control in adult diabetes. Diabetes Metab Syndr Obes Target Ther 4:53–60Google Scholar
- Kuby J (1994) Immunology, W.H. Freeman, (2ndedn), New York, NY, USAGoogle Scholar
- Malik F, Singh J, Khajuria A, Suri KA, Satti NK, Singh S, Kaul MK, Kumar A, Bhatia A, Qazi GN (2007) A standardized root extract of Withania somnifera and its major constituent withanolide-A elicit humoral and cell-mediated immune responses by up regulation of Th1-dominant polarization in BALB/c mice. Life Sci 80:1525–1538CrossRefPubMedGoogle Scholar
- Muthamizhe Selvan K, Prakash YG, Gopal V (2013) Standardization of traditional medicine - Need and Urgency. Inter J Phytotherapy 3:5–10Google Scholar
- Nagalakshmi D, Sridhar K, Parashuramulu S (2015) Replacement of inorganic zinc with lower levels of organic zinc (zinc nicotinate) on performance, hematological and serum biochemical constituents, antioxidants status and immune responses in rats. Vet World 8:1156–1162CrossRefPubMedPubMedCentralGoogle Scholar
- Nelson DA, Mildenhall P (1967) Studies on cytophilic antibodies. The production by mice of macrophage cytophilic antibodies to sheep erythrocytes: Relationship to the production of other antibodies and development of delayed type hypersensitivity. Aust J Exp Bio Med Sci 45:113–120CrossRefGoogle Scholar
- Panda AK, Rout R (2006) Zinc in Ayurvedic herbo-mineral products. Nat Prod Radiance 5:284–288Google Scholar
- Qayyum R, Kurbanova N, Zia R, Adomaityte J (2014) Serum selenium levels are associated with blood platelet count in US Adults. Meeting: SHM Annual MeetingGoogle Scholar
- WHO (1993) Research Guideline for Evaluating the Safety and Efficacy of Herbal Medicines. World Health Organization, Manila, p 2Google Scholar