Oriental Pharmacy and Experimental Medicine

, Volume 16, Issue 2, pp 147–152 | Cite as

Analgesic, anthelmintic and toxicity studies of Solanum violaceum Linn. Leaves

  • Kamanashis Mahaldar
  • Md. Saifuzzaman
  • Tanzira Irin
  • Apurba Kumar Barman
  • Md. Khirul Islam
  • Md. Mustafizur Rahman
  • Md. Amirul Islam
Short Communication


The methanolic extract of Solanum violaceum L. (Solanaceae), commonly known as Indian Nightshade, has been subjected to analgesic, anthelmintic and acute toxicity studies. Acute toxicity was examined for a period of seven days at doses of 2.0 g/kg (i.p.) and 5.0 g/kg (p.o.) in mice. Analgesic activity (250 and 500 mg/kg, p.o.) was assessed following acetic acid- and hot plate-induced pain on mice model. Live parasites Paramphistomum cervi Z. (Paramphistomatidae) and Haemonchus contortus R. (Trichostrongylidae) were used to evaluate anthelmintic activity at concentrations of 25, 50, 100 and 200 mg/mL. The extract showed no toxicity sign at both of the doses. In analgesic tests, extract inhibited 26 % and 58 % abdominal constriction at doses of 250 and 500 mg/kg, respectively, and significantly (P < 0.01) raised pain threshold at both doses. Fastest paralysis occurred in both species of helminths at higher concentrations (100 and 200 mg/mL). The relative index values for paralysis in H. contortus were 1.69, 1.04, 0.57 and 0.31 at the used concentrations mention above. The relative index of death in H. contortus suggested that S. violaceum is parasiticidal at high concentration. Likewise, relative indexes for paralysis and death in P. cervi proposed that S. violaceum is strong parasiticidal agent and comparable with albendazole. These results corroborate the traditional uses of S. violaceum in analgesia and helminthiasis and explain it on scientific grounds.


Acetic acid induced writhing Haemonchus contortus Hot-plate test Mice model Parasiticidal Paramphistomum cervi Solanum violaceum 



The authors are grateful to the authorities of Pharmacy Discipline, Life Science School, Khulna University, Bangladesh for providing financial and instrumental facilities. We also like to thank the authority of Bangladesh National Herbarium for identification of the plant.

Compliance with ethical standards

Ethical Statement

The research was carried out according to the rules governing the use of laboratory animals as acceptable internationally and the experimental protocol was approved by the Animal Ethics Committee, Khulna University (Ref: AEC/02/2004).

Conflict of Interest

The authors declare that they have no competing interests.


  1. Acharya S, Dash GK, Brahma DK, Chhetree RR (2011) Preliminary phytochemical investigarion and chemical activity of Acacia suma (Roxb) barks. Int Res J Pharm 2:136–141Google Scholar
  2. Ahmed F, Selim MST, Das AK, Choudhuri MSK (2004) Anti-inflammatory and antinociceptive activities of Lippia nodiflora Linn. Pharmazie 59:329–330PubMedGoogle Scholar
  3. Athnasiadau S, Kyriazakis I, Jackson F, Coop RL (2001) Direct anthelmintic effects of condensed tannins towards different gastrointestinal nematodes of sheep: In vitro and in vivo studies. Vet Parasitol 99:205–219CrossRefGoogle Scholar
  4. Azando EV, Hounzangbé-Adoté MS, Olounladé PA, Brunet S, Fabre N, Valentin A, Hoste H (2011) Involvement of tannins and flavonoids in the in vitro effects of Newbouldia laevis and Zanthoxylum zanthoxyloides extracts on the exsheathment of third-stage infective larvae of gastrointestinal nematodes. Vet Parasitol 180:292–297CrossRefPubMedGoogle Scholar
  5. Chang FR, Yen CT, El-Shazly M, Yu CY, Yen MH, Cheng YB, Chen SL, Wu YC (2013) Spirostanoids with 1,4-dien-3-one or 3β,7α-diol-5,6-ene moieties from Solanum violaceum. Bioorg Med Chem Lett 23:2738–2742CrossRefPubMedGoogle Scholar
  6. Deraedt R, Jouquey S, Delevallee F, Flahaut M (1980) Release of prostaglandins E and F in an algogenic reaction and its inhibition. Eur J Pharmacol 61:17–24CrossRefPubMedGoogle Scholar
  7. de Sá PG, Nunes XP, de Lima JT, de Siqueira Filho JA, Fontana AP, Siqueira Jde S, Quintans-Júnior LJ, Damasceno PK, Branco CR, Branco A, Almeida JR (2012) Antinociceptive effect of ethanolic extract of Selaginella convoluta in mice. BMC Complement Altern Med 12:187CrossRefPubMedPubMedCentralGoogle Scholar
  8. dos Santos DA, Fukui Mde J, Dhammika Nanayakkara NP, Khan SI, Sousa JP, Bastos JK, Andrade SF, da Silva Filho AA, Quintão NL (2010) Anti-inflammatory and antinociceptive effects of Baccharis dracunculifolia DC (Asteraceae) in different experimental models. J Ethnopharmacol 127:543–550CrossRefPubMedGoogle Scholar
  9. Duarte ID, Nakamura M, Ferreira SH (1988) Participation of the sympathetic system in acetic acid-induced writhing responses in mice. Braz J Med Biol Res 21:341–343PubMedGoogle Scholar
  10. Ghani A (2003) Medicinal plants of Bangladesh: chemical constituents and uses. Dhaka, BangladeshGoogle Scholar
  11. Goto C , Kasuya S, Koga K, Ohtomo H, Kagel N (1990). Lethal efficacy of extract from Zingiber officinale (traditional Chinese medicine) or [6]-shogaol and [6]-gingerol in Anisakis larvae in vitro. Parasitol Res 76:653–656.CrossRefPubMedGoogle Scholar
  12. Herrera-Arellano A, Jiménez-Ferrer E, Vega-Pimentel AM, Martínez-Rivera ML, Hernández-Hernández M, Zamilpa A, Tortoriello J (2004) Clinical and mycological evaluation of therapeutic effectiveness of Solanum chrysotrichum standardized extract on patients with Pityriasis capitis (dandruff). A double blind and randomized clinical trial controlled with ketoconazole. Planta Med 70:483–488CrossRefPubMedGoogle Scholar
  13. Hossain E, Chandra G, Nandy AP, Mandal SC, Gupta JK (2012) Anthelmintic effect of a methanol extract of Bombax malabaricum leaves on Paramphistomum explanatum. Parasitol Res 110:1097–1102CrossRefPubMedGoogle Scholar
  14. Ikeda Y, Ueno A, Naraba H, Oh-ishi S (2001) Involvement of vanilloid receptor VR1 and prostanoids in the acid induced writhing responses of mice. Life Sci 69:2911–2919CrossRefPubMedGoogle Scholar
  15. Islam MA, Ahmed F, Das AK, Bachar SC (2005) Analgesic and anti-inflammatory activity of Leonurus sibiricu. Fitoterapia 76:359–362CrossRefPubMedGoogle Scholar
  16. Parke DV, Sapota A (1996) Chemical toxicity and reactive species. Int J Occup Med Environ Health 9:119–123Google Scholar
  17. Pathak D, Pathak K, Singla AK (1991) Flavonoids as medicinal agents, recent advances. Fitoterapia 62:371–389Google Scholar
  18. Rajnarayana K, Reddy MS, Chaluvadi MR, Krishna DR (2001) Bi-flavonoids classification, pharmacological, biochemical effects and therapeutic potential. Indian J Pharm 33:2–16Google Scholar
  19. Raju GS, Moghal MM, Dewan SM, Amin MN, Billah MM (2013) Characterization of phytoconstituents and evaluation of total phenolic content, anthelmintic and antimicrobial activities of Solanum violaceum Ortega. Avicenna J Phytomed 3:313–320PubMedPubMedCentralGoogle Scholar
  20. Rao MR, Rao YM, Rao AV, Prabhkar MC, Rao CS (1998) Antinociceptive and anti-inflammatory activity of a flavonoid isolated from Caralluma attenuate. J Ethnopharmacol 62:63–66CrossRefPubMedGoogle Scholar
  21. Ribeiro RA, Vale ML, Thomazzi SM, Paschoalato AB, Poole S, Ferreira SH, Cunha FQ (2000) Involvement of resident macrophages and mast cells in the writhing nociceptive response induced by zymosan and acetic acid in mice. Eur J Pharmacol 387:111–118CrossRefPubMedGoogle Scholar
  22. Robinson RD, Williams LA, Linda JF, Terzy SI, Mansingh A (1990) Inactivation of Strongyloides stercoralis larvae in vitro by six Jamaican plant extracts and three commercial anthelmintics. West Indian Med J 39:213–217PubMedGoogle Scholar
  23. Saifuzzaman M, Shamim MSJ, Mahaldar K, Ali ES, Islam MA (2013) Antinociceptive activity of the ethanolic extract of Persicaria acuminata Sach. J Pharm Res 6:753–755Google Scholar
  24. Saha S, Shilpi JA, Mondal H, Gofur R, Billah M, Nahar L, Sarker SD (2013) Bioactivity studies on Musa seminifera Lour. Pharmacogn Mag 9:315–322CrossRefPubMedPubMedCentralGoogle Scholar
  25. Tandon V, Pal P, Roy B, Rao HS, Reddy KS (1997) In vitro anthelmintic activity of root-tuber extract of Flemingia vestita, an indigenous plant in Shillong, India. Parasitol Res 83:492–498CrossRefPubMedGoogle Scholar
  26. Thompson DP, Geary TG (1995). The structure and function of helminth surfaces. In: Marr J & Muller M (ed) Biochemistry and Molecular Biology of Parasites. New York, pp 203–32.Google Scholar
  27. Togo J, Santamarina MT, Peris D, Ubeira FM, Leiro SL, Sanmartin ML (1992) In vitro effect of anthelmintics on Anisakis simplex survival. Jpn J Parasitol 41:473–480Google Scholar
  28. Vongtau HO, Abbah J, Mosugu O, Chindo BA, Nagazal IE, Salawu AO, Kwanashie HO, Gamaniel KS (2004) Antinociceptive profile of the methanol extract of Neorautanenia mitis root in rats and mice. J Ethnopharmacol 92:317–324CrossRefPubMedGoogle Scholar
  29. Wang GX, Han J, Zhao LW, Jiang DX, Liu YT, Liu XL (2010) Anthelmintic activity of steroidal saponins from Paris polyphylla. Phytomedicine 17:1102–1105CrossRefPubMedGoogle Scholar
  30. Yen CT, Lee CL, Chang FR, Hwang TL, Yen HF, Chen CJ, Chen SL, Wu YC (2012) Indiosides G-K: steroidal glycosides isolated from Solanum violaceum with cytotoxic and anti-inflammatory activities. J Nat Prod 75:636–643CrossRefPubMedGoogle Scholar

Copyright information

© Institute of Korean Medicine, Kyung Hee University and Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Kamanashis Mahaldar
    • 1
  • Md. Saifuzzaman
    • 1
    • 2
  • Tanzira Irin
    • 1
  • Apurba Kumar Barman
    • 1
    • 3
  • Md. Khirul Islam
    • 1
    • 4
  • Md. Mustafizur Rahman
    • 1
  • Md. Amirul Islam
    • 1
  1. 1.Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Life Science SchoolKhulna UniversityKhulnaBangladesh
  2. 2.School of Molecular Science, Faculty of Science, Technology & EngineeringLa Trobe UniversityBendigoAustralia
  3. 3.Institute of Biophysics Key Laboratory of Protein and Peptide DrugsUniversity of Chinese Academy of SciencesBeijingChina
  4. 4.Biochemistry DepartmentUniversity of TurkuTurkuFinland

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