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Oriental Pharmacy and Experimental Medicine

, Volume 14, Issue 3, pp 285–289 | Cite as

Hepatoprotective activity of moralbosteroid, a steroidal glycoside isolated from Morus alba

  • Gaurav GuptaEmail author
  • Rohit Verma
  • Sheba Rani David
  • Dinesh Kumar Chellappan
  • Firoz Anwar
  • Kamal Dua
Short Communication

Abstract

This study evaluates the hepatoprotective activity of moralbosteroid, isolated from Morus alba, against the hepatotoxicity induced by CCl4 in wistar albino rats. The level of hepatoprotection was estimated by measuring the following biochemical markers: aspartate amino-transferase (AST), alkaline phosphatase (ALP), serum alanine amino-transferase (ALT), total bilirubin (TB), and total protein (TP), including the enzymes involved in antioxidant activities like glutathione transferase (GST), glutathione peroxidase (GPx), catalase (CAT), lipid peroxidation (LPO) and superoxide dismutase (SOD). The oral administration of CCl4 significantly caused elevation in LPO level (13.22 ± 1.59 μM/mg protein) as compared to control. The activities of antioxidant enzymes including CAT, SOD, GPx and GST were decreased significantly (0.38 ± 0.6 nmol/min/ml, 0.89 ± 0.83 U/ml, 3.90 ± 0.91 μmol and 0.05 ± 0.16 U/min/mg protein) in testicular tissue as compared to control animals. Moralbosteroid significantly prevents the marked escalation of serum markers and inhibited the free radical processes by the scavenging of hydroxyl radicals. It also modulates the levels of LPO and prominently increases the endogenous antioxidant enzyme levels in hepatocellular toxicity induced by CCl4. The results obtained in the present study suggest the preventive influence of moralbosteroid on liver toxicity in rats induced by CCl4 comparable with those of Silymarin.

Keywords

Morus alba Moralbosteroid Carbon tetrachloride Silymarin Hepatoprotective Rats 

Notes

Conflict of interest

Authors Gaurav Gupta, Rohit Verma, Sheba Rani David, Dinesh Kumar Chellappan, Firoz Anwar, and Kamal Dua certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript title “Hepatoprotective activity of moralbosteroid, a steroidal glycoside isolated from Morus alba”.

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Copyright information

© Institute of Korean Medicine, Kyung Hee University 2014

Authors and Affiliations

  • Gaurav Gupta
    • 1
    • 2
    Email author
  • Rohit Verma
    • 2
  • Sheba Rani David
    • 2
  • Dinesh Kumar Chellappan
    • 2
  • Firoz Anwar
    • 3
  • Kamal Dua
    • 2
  1. 1.Pacific UniversityUdaipurIndia
  2. 2.School of PharmacyInternational Medical UniversityKuala LumpurMalaysia
  3. 3.Department of PharmacologySiddhartha Institute of PharmacyDehradunIndia

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