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Oriental Pharmacy and Experimental Medicine

, Volume 14, Issue 2, pp 127–144 | Cite as

In vivo assessment of antidiabetic and antioxidant activities of methanol extract of Smilax zeylanica leaves in wistar rats

  • Venugopalan Rajesh
  • Perumal Perumal
Research Article

Abstract

The aim of the present study was to evaluate the antidiabetic and antioxidant effect of methanol extract of Smilax zeylanica leaves on streptozotocin (STZ) induced diabetic rats. Experimental diabetes was induced by a single intraperitoneal (i.p.) injection of STZ (60 mg/kg body weight) dissolved in 0.1 M cold citrate buffer (pH 4.5). Diabetic rats exhibited increased plasma glucose levels with significant decrease in serum insulin levels. There was an apparent reduction in body weight and significant increase in aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total bilirubin levels with concomitant decrease in serum protein levels. In addition, there was significant elevation in serum urea and creatinine levels in diabetic control animals compared to the control animals. Moreover, with reference to lipid peroxidation and antioxidant systems, there was significant increase in levels of lipid peroxidation in liver and kidney tissues of diabetic animals with concomitant decrease in activities of antioxidant enzymes viz., superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione-S-transferase (GST) and non-enzymatic antioxidants glutathione (GSH), vitamin- C and vitamin-E in liver and kidney tissues. Treatment with Smilax zeylanica leaf extract at dose levels of 200 mg/kg and 400 mg/kg for a period of 28 days showed a significant ameliorative effect on all biochemical parameters studied. The extract treatment also increased the body weight of diabetic rats significantly compared to diabetic control rats. On other hand, the extract treatment decreased the extent of lipid peroxidation and was able to normalize the activities of enzymatic and non-enzymatic antioxidants in liver and kidney tissues. Histological examination of pancreas, liver and kidney too correlated with biochemical observations. Furthermore, the extract did not produce any deleterious effects in extract alone treated groups. The phytochemical and quantitative analysis revealed that the extract was rich in total phenolics and flavonoids. These results suggest that, the antidiabetic and antioxidant potential of Smilax zeylanica leaf extract might be due to stabilization and increase in all the components of antioxidant system attributed to antioxidant and free radical scavenging activity.

Keywords

Smilax zeylanica leaves Streptozotocin Antidiabetic Lipid peroxidation Antioxidant activity 

Notes

Acknowledgments

The authors are thankful to the management of JKK Nattraja College of Pharmacy, Komarapalayam, Tamil Nadu, INDIA for providing necessary facilities to carry out the research work.

References

  1. Abdala S, Martin-Herrera D, Benjumea D, Perez-Paz P (2008) Diuretic activity of Smilax canariensis, an endemic canary Islend species. J Ethnopharmacol 119:12–16PubMedCrossRefGoogle Scholar
  2. Allen RG (1991) Oxygen-reactive species and antioxidant responses during development: the metabolic paradox of cellular differentiation. Proc Soc Exp Biol Med 196:117–129PubMedCrossRefGoogle Scholar
  3. Almadal TP, Vilstrup H (1988) Strict insulin treatment normalizes the organic nitrogen contents and the capacity of urea-N synthesis in experimental diabetes in rats. Diabetologica 31:114–118CrossRefGoogle Scholar
  4. Alvarez JF, Barbera A, Nadal B, Barcclo-Ballori S, Piquer S, Claret M, Guinovart JJ, Guinovart N, Gomis R (2004) Stable and functional regeneration of pancreatic β cell population in n-STZ rats treated with tungstate. Diabetologia 47:470–477CrossRefGoogle Scholar
  5. Ambasta SP (2006) The useful plants of India. New Delhi. NISCAIR: 578Google Scholar
  6. Atli A, Keven K, Avci A, Kutlay S, Turkcapar M, Varli S, Aras S, Ertug E, Canbolat O (2004) Oxidative stress and antioxidant status in elderly diabetes mellitus and glucose intolerance patients. Arch Gerontol Geriatr 39:269–275PubMedCrossRefGoogle Scholar
  7. Barker H, Frank O, Angelis B, Feingold S (1951) Plasma tocopherol in man at various times after ingesting free or acetylated tocopherol. Nutr Rep Int 21:531–536Google Scholar
  8. Bebe FN, Panemangalore M (2003) Exposure to low doses of endosulfan and chlorpyrifos modifies endogenous antioxidants in tissues of rats. J Environ Sci Health B38:349–363CrossRefGoogle Scholar
  9. Bhattacharjee R, Sil PC (2006) The protein fraction of Phyllanthus niruri plays a protective role against acetaminophen induced hepatic disorder via its antioxidant properties. Phytother 20:595–601CrossRefGoogle Scholar
  10. Chang C, Yang M, Wen H, Chen J (2002) Estimation of total flavonoids content in propolis by two complementary colorimetric methods. J Food Drug Anal 10:178–182Google Scholar
  11. Chaudiere J (1994) Some chemical and biochemical constrains of oxidative stress in living cells. In: Rice-Evans CA, Burdon RH (eds) Free radical damage and its control. Elsevier Science, Amsterdam, pp 25–26CrossRefGoogle Scholar
  12. Coskun O, Kanter M, Korkmaz A, Oter S (2008) Quercetin, a flavonoid antioxidant, prevents and protects streptozotocin-induced oxidative stress and b-cell damage in rat pancreas. Food Chem 108:965–972CrossRefGoogle Scholar
  13. Das S (1994) Vitamin E in the genesis and prevention of cancer. A review. Acta Oncol 33:615–619PubMedCrossRefGoogle Scholar
  14. Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70–77PubMedCrossRefGoogle Scholar
  15. Erejuwa OO, Sulaiman SA, Wahab MS, Salam SK, Salleh MS, Gurtu S (2010) Antioxidant protective effect of glibenclamide and metformin in combination with honey in pancreas of streptozotocin-induced diabetic rats. Int J Mol Sci 11:2056–2066PubMedCentralPubMedCrossRefGoogle Scholar
  16. Evans WC (2002) Trease and Evans pharmacognosy. London, Saunders. pp 300,480Google Scholar
  17. Farombi EO, Olowg BI, Emerole GO (2000) Effect of three structurally related antimalarial drugs on liver microsomal components and lipid peroxidation in rats. Comp Biochem Physiol 126:217–224Google Scholar
  18. Folch J, Lees M, Solane SGH (1957) A simple method for isolation and purification of total lipids from animal tissues. J Bio Chem 26:497–509Google Scholar
  19. Freeman BA, Crapo JD (1982) Biology of disease. Free radicals and tissue injury. Lab Invest 47:412–426PubMedGoogle Scholar
  20. Gamble JS (2004) Flora of the presidency of Madras. Dehradun: Bishen Singh, Mahendra Pal Singh eds, pp 1518Google Scholar
  21. George J (2003) Ascorbic acid concentrations in diethylnitrosamine induced hepatic fibrosis in rats. Clin Chem Acta 335:39–47CrossRefGoogle Scholar
  22. Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione S transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139PubMedGoogle Scholar
  23. Horn HD, Burns FH (1978) Assay of glutathione reductase activity. In: Bergmeyer HV (ed) Methods of enzymatic analysis. Academic, New York, pp 142–146Google Scholar
  24. Kakkar P, Das B, Viswanath PN (1984) Modified spectrophotometer assay of SOD. Ind J Biochem Biophys 95:51–58Google Scholar
  25. Katiyar SK, Agarwal R, Mukhtar H (1993) Protective effects of green tea polyphenols administration by oral intubation against chemical carcinogen induced forestomach and pulmonary neoplasia in A/J mice. Cancer Lett 73:167–172PubMedCrossRefGoogle Scholar
  26. Kawamura N, Oakawara T, Suzuki K, Konishi K, Mino M, Taniguchi N (1992) Increased glycated Cu, Zn-superoxide dismutase levels in erythrocytes of patients with insulin-dependent diabetes mellitus. J Clin Endocrinol Metab 74:1352–1354PubMedGoogle Scholar
  27. Khandelwal KR, Kokate CK (1995) Pratical pharmacognosy, 4th edn. Vallabh Prakashan, New Delhi, p 110Google Scholar
  28. Kind PRN, King EJJ (1954) Estimation of plasma phosphatase by determination of hydrolysed phenol with antipyrine. J Clin Pathol 7:322–330PubMedCentralPubMedCrossRefGoogle Scholar
  29. Kirtikar KR, Basu BD (1991) Indian medicinal plants. Dehra Dun: Bishen Singh, Mahendra Pal Singh, eds, pp 2496Google Scholar
  30. Shijun L, Tao Y, Yang J-Q, Terry DO, Larry WO (2000) The role of cellular glutathione peroxidase redox regulation in the suppression of tumour cell growth by manganese superoxide dismutase. Cancer Res 60:3927–3939Google Scholar
  31. Malloy HT, Evelyn KA (1937) The determination of bilirubin with the photometric colorimeter. J Biol Chem 119:481–490Google Scholar
  32. Mary E, Waltner L, Xiaokui L, Wang Brain KL, Robert KH, Nawano M, Granner DK (2002) Epigallocatechin gallate, a constituent of green tea, suppresses hepatic glucose production. J Biol Chem 277:34933–34940CrossRefGoogle Scholar
  33. Morgan PE, Dean RT, Davies MJ (2002) Inactivation of cellular enzymes by carbonyls and protein-bound glycation/glycoxidation products. Arch Biochem Biophys 403:259–269PubMedCrossRefGoogle Scholar
  34. Murray RK, Granner DK, Mayes PA, Rodwell VW (2003) Harper’s Illustrated Biochemistry, 26th edn. The McGraw-Hill Companies Inc.Google Scholar
  35. Nadkarni KM (1976) Indian materica medica. Bombay Popular Prakashan, Bombay, p 1145Google Scholar
  36. Nwanjo HU, Oze GO (2007) Oxidative imbalance and non-enzymic antioxidant status in pulmonary tuberculosis infected. Pak J Nutr 6:590–592CrossRefGoogle Scholar
  37. OECD (2000) Guidance document on acute oral toxicity 425. Environmental health and safety monograph series on testing assessment. NO. 24Google Scholar
  38. Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358PubMedCrossRefGoogle Scholar
  39. Omaye ST, Turnbull JD, Sauberlich HE (1979) Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids. Methods Enzymol 62:1–11Google Scholar
  40. Oommachan, Masih SK (1991) Ethnobotanical and conservational aspects of medicinal plants of Madhya Pradesh. Indian J Pure Applied Biol 6(1):39–44Google Scholar
  41. Pandit R, Phadke A, Jagtap A (2010) Antidiabetic effect of Ficus religiosa extract in streptozotocin-induced diabetic rats. J Ethnopharmacol 128:462–466PubMedCrossRefGoogle Scholar
  42. Patton CJ, Crouch SR (1977) Spectrophotometric and kinetics investigation of the Berthelot reaction for the determination of ammonia. Anal Chem 49:464–469CrossRefGoogle Scholar
  43. Polshettiwar SA, Ganjiwale RO, Wadher SJ, Yeole PG (2007) Spectrophotometric estimation of total tannins in some ayurvedic eye drops. Indian J Pharma Sci 69:574–576CrossRefGoogle Scholar
  44. Praveen K, Khan MR, Mujeeb M, Siddiqui WA (2010) Protective effects of pycnogenol on hyperglycemia induced oxidative damage in the liver of type 2 diabetes rats. Chem Biol Interact 186:219–227CrossRefGoogle Scholar
  45. Ramaswamy SN, Radhakrishna Rao M, Govindappa (2001) Flora of Shimoga district Karnataka. Prasaranga, Mysore, p 619Google Scholar
  46. Reitman S, Frankel SA (1957) Coloirmetric method for determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am J Clin Pathol 28:56–63PubMedGoogle Scholar
  47. Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG (1973) Selenium: biochemical role as a component of glutathione peroxidase. Science 179:588–590PubMedCrossRefGoogle Scholar
  48. Saldhana CJ, Wicolson DH (1976) Flora of Hassan district of Karnataka. Amerind Pub Co Pvt Ltd, New Delhi, p 804Google Scholar
  49. Sallie R, Tredger JM, Willam R (1991) Drug and the liver. Biopharm Drug Dispos 12:251–259PubMedCrossRefGoogle Scholar
  50. Santapau H, Henry AN (1976) A dictionary of the flowering plants in India (reprint). CSIR, New Delhi, p 58Google Scholar
  51. Sen S (1984) Smilax zeylanica Linn - A new source of diosgenin. Curr Sci 53(12):661Google Scholar
  52. Shao B, Guo HZ, Cui YJ, Ye M, Han J, Guo DA (2007) Steroidal saponins from Smilax china and their anti-inflammatory activities. Phytochemistry 68:623–630PubMedCrossRefGoogle Scholar
  53. Siegers CP, Younes M (1981) Effect of bioflavonoids on lipid peroxidation induced by glutathione depletion. Pro Int Bioflavonoid Symposium, Munich, FRG, pp 409Google Scholar
  54. Siest G, Henny J, Schiele F, Young DS (1985) Kinetic determination of creatinine. Interpretation of clinic lab tests. Biomedical publications, Foster City, pp 220–224Google Scholar
  55. Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viticult 16:144–158Google Scholar
  56. Sinha AK (1972) Colorimetric assay of catalase. Anal Biochem 47:389–394PubMedCrossRefGoogle Scholar
  57. Slinkard K, Singleton VL (1977) Total phenol analysis: automation and comparison with manual methods. Am J Enol Viticult 28:49–55Google Scholar
  58. Swanston-Flatt SK, Day C, Bailey CJ, Flatt PR (1990) Traditional plant treatments for diabetes: studies in normal and streptozotocin diabetic mice. Diabetologia 33:462–464PubMedCrossRefGoogle Scholar
  59. Thulesen J, Orskov C, Holst JJ, Poulsen SS (1997) Short-term insulin treatment prevents the diabetogenic action of streptozotocin in rats. Endocrinology 138:62–68PubMedGoogle Scholar
  60. Triender P (1969) Determination of glucose using glucose oxidase with an alternative oxygen acceptor. Annu Clin Biochem 6:24–27CrossRefGoogle Scholar
  61. Wang XJ, Feng P, Wen ZY (1996) Study on invitro and invivo anticancer action of compound Smilax china L. Chin J Pathophysiol 12:614–1614Google Scholar
  62. Wiseman H (1996) Dietary influences on membrane function: importance in protection against oxidative damage and disease. J Nutr Biochem 7:2–15CrossRefGoogle Scholar
  63. Wooten IDP (1964) Micro-analysis in medical biochemistry, 4th edn. J and A Churchill Ltd, London, pp 138–140Google Scholar
  64. Wroblewski F (1959) The clinical significance of transaminase activities of serum. Am J Med 27:911–923PubMedCrossRefGoogle Scholar

Copyright information

© Institute of Korean Medicine, Kyung Hee University 2013

Authors and Affiliations

  1. 1.Department of PharmacologyJKK Nattraja College of PharmacyNamakkal DistrictIndia

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