In-vivo immunomodulatory activity of standardized Stereospermum suaveolens (Roxb.) DC. root extract

  • Amal K. Maji
  • Sourav K. Samanta
  • Subhadip Mahapatra
  • Pratim Banerji
  • Debdulal Banerjee
Research Article

Abstract

Stereospermum suaveolens (Roxb.) DC. is used in various Ayurvedic formulations to treat variety of disorders including inflammations, asthma, blood disorders, fevers, liver disorders etc. Quantification of the bioactive compound in S. suaveolens root extract (SSRE) was determined through RP-HPLC, in order to standardize the plant material with optimal concentration of known active constituents present there in. The immunomodulatory potential of SSRE was determined for its effects on non-specific immune functions against sheep red blood cells antigenic challenge using in-vivo models. The assay included total and differential leukocyte counts, nitroblue-tetrazolium reduction test, neutrophil adhesion test, phagocytic activity and delayed type hypersensitivity (DTH) reaction. In RP-HPLC analysis, the contents of dehydro-α-lapachone and lapachol in SSRE was found to be 0.043 ± 0.003 and 0.16 ± 0.002 % (w/w), respectively. Standardized SSRE (100–300 mg/kg) increased the total leukocyte count and the population of monocyte and neutrophil in rats. Further, treatment with SSRE increased the neutrophil adhesion to nylon fibres, DTH response, phagocytic activity and intracellular killing potential of phagocytes in a dose dependent manner. The immunostimulatory potential of SSRE at 300 mg/kg was found to be very significant (p < 0.001) in compared to the control. These findings suggest that SSRE can able to stimulate the innate defence mechanisms of an individual and it can be considered as an alternative therapy to boost the innate immune functions during the impaired immunological conditions.

Keywords

Stereospermum suaveolens RP-HPLC Immunomodulatory activity 

Notes

Acknowledgments

The authors are thankful to Ulysses Research Foundation, India for providing financial assistance to carry out this research work and University Grants Commission (UGC), New Delhi for SAP assistance to Department of Botany, VU.

Conflict of interest

There are no known conflicts of interest associated with this publication.

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Copyright information

© Institute of Korean Medicine, Kyung Hee University 2013

Authors and Affiliations

  • Amal K. Maji
    • 1
  • Sourav K. Samanta
    • 2
  • Subhadip Mahapatra
    • 1
  • Pratim Banerji
    • 3
  • Debdulal Banerjee
    • 1
  1. 1.Department of Botany and ForestryVidyasagar UniversityMidnaporeIndia
  2. 2.School of Natural Product Studies, Department of Pharmaceutical TechnologyJadavpur UniversityKolkataIndia
  3. 3.Ulysses Research FoundationKolkataIndia

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