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Oriental Pharmacy and Experimental Medicine

, Volume 14, Issue 2, pp 103–109 | Cite as

In vivo evaluation of cutaneous wound healing activity of Mirabilis jalapa L radix

  • Jyotchna Gogoi
  • Khonamai Sewa Nakhuru
  • Pronobesh Chattophadhayay
  • Ashok Kumar Rai
  • Hemanta Kumar Gogoi
  • Vijay Veer
Research Article

Abstract

The present study was to evaluate in vivo cutaneous wound healing activity of hydromethanolic extract of M. jalapa radix and to validate its traditional use as medicine for wound healing. In excision wound model, the wound area was measured on the day of infliction (zero day) and subsequently at 4 days interval until the completion of wound healing. In dead space wound model, on 10th post wounding day, the granulation tissues were harvested for estimation of blotting of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (FGF 2) and collagen type III (COL 3A), hydroxyproline content and histological studies. A significant increase (p < 0.05) in rate of wound contraction, decrease in Colony Forming Unit (CFU) count and decrease in epithelisation period was observed in 5 and 10 % treated groups as compared to control. A significant increase (p < 0.05) in hydroxyproline content, up- regulated expression of COL 3A was observed in treated groups as compared to control which was supported by histological evidences. Increase in rate of wound contraction, hydroxyproline content and COL 3A, decrease in epithelisation period and CFUs count shows the wound healing activity of M.jalapa and validate its traditional use in wound and inflammation.

Keywords

Epithelialisation period Granulation Hydroxyproline Mirabilis jalapaWound healing 

Notes

Acknowledgments

Authors are highly thankful to DRDO, New Delhi for the financial support and Semima Yeshim, Department of Life Sciences, Dibrugarh University, for her help in wound tissue sectioning.

Conflict of interest

None

References

  1. Bradford MM (1976) Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254PubMedCrossRefGoogle Scholar
  2. Burnette WN (1981) “Western blotting” : electrophoretic transfer of proteins from sodium dodecyl sulphate−polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem 112:195–203PubMedCrossRefGoogle Scholar
  3. Copenhaver WM, Bunge RP, Bunge MB (1967) Bailey’s textbook of histology, 16th edn. Williams &WilkinsGoogle Scholar
  4. Cristiani IBW, Gabriela T, Mateus FR, Carina F, Maria EP, Juliano F, Melânia PM (2008) Antinociceptive activity of Mirabilis jalapa in mice. J Ethnopharmacol 120:169–175CrossRefGoogle Scholar
  5. Diegelmann RF, Evans MC (2004) Wound healing: an overview of acute, fibrotic and delayed healing. Front Biosci 9:283–289PubMedCrossRefGoogle Scholar
  6. Evans WC (1997) Trease and Evans pharmacognosy, 14th edn. Harcourt Brace and company:Asia Pvt. Ltd. SingaporeGoogle Scholar
  7. Fatima AD, Modolo LV, Sanches ACC, Porto RR (2008) Wound healing agents: the role of natural and non-natural products in drug development. Mini Rev Med Chem 8:879–888PubMedCrossRefGoogle Scholar
  8. Ferrara N (1999) Molecular and biological properties of vascular endothelial growth factor. J Mol Med 77:527–543PubMedCrossRefGoogle Scholar
  9. Gogoi J, Nakhuru KS, Rai AK, Chattophadhayay P, Gogoi HK, Singh L (2012) Antimicrobial and free radical scavenging activities of crude extract and fractions from the tuberous root of Mirabilis jalapa L. South Asian J Exp Biol 2:209–216Google Scholar
  10. Goldfarb M (2001) Signaling by fibroblast growth factors: the inside story. doi: 10.1126/stke.2001.106.pe37
  11. Gutierrez RMP, Vargas SR (2006) Evaluation of the wound healing properties of Acalypha langiana in diabetic rats. Fitoterapia 77:286–289CrossRefGoogle Scholar
  12. Joukov V, Sorsa T, Kumar V, Jeltsch M, Claesson-Walsh L, Cao Y, Saksela O, Kalkkinen N, Alitalo K (1997) Proteolytic processing regulates receptor specificity and activity of VEGF – C. EMBO J 16:3898–3911PubMedCentralPubMedCrossRefGoogle Scholar
  13. Kokate CK (1999) Practical Pharmacognosy, 4th edn. Vallabh Prakashan Publication, New DelhiGoogle Scholar
  14. Kumar R, Katoch SS, Sharma S (2006) β-Adrenoceptor agonist treatment reverses denervation atrophy with augmentation of collagen proliferation in denervated mice gastrocnemius muscle. Indian J Exp Biol 44:371–376PubMedGoogle Scholar
  15. Lobb RR, Alderman EM, Fett JW (1985) Induction of angiogenesis by bovine brain derived class 1 heparin-binding growth factor. Biochem 24:4969–4973CrossRefGoogle Scholar
  16. Mace ME (1963) Histochemical localization of phenols in healthy and diseased tomato roots. Phytopathol 16:915–925Google Scholar
  17. Maquart FX, Chastang F, Simeon A, Birembaut P, Gillery P, Wergrowski Y (1999) Triterpenes from Centella asiatica stimulate extracellular matrix accumulation in rat experimental wounds. Eur J Dermatol 9:289–296PubMedGoogle Scholar
  18. Mohamed H, Jarraya R, Lassoued I, Masmoudi O, Damak M, Moncef N (2010) GC/MS and LC/MS analysis, antioxidant and antimicrobial activities of various solvent extracts from Mirabilis jalapa tubers. Process Biochem 45:1486–1493CrossRefGoogle Scholar
  19. Morton JP, Melone MH (1972) Evaluation of vulnerary activityby an open wound procedure in rats. Arch Int Pharmacodyn 196:117–120PubMedGoogle Scholar
  20. Mukherjee KL (2000) Medical Laboratory Technology. Tata McGraw Hill Ltd, New DelhiGoogle Scholar
  21. Neumann RE, Logon MA (1950) The determination of collagen and elastin in tissues. J Biol Chem 186:549–556Google Scholar
  22. Pfoze NL, Kumar Y, Myrboh B (2012) Survey and assessment of ethnomedical plants used in Senapati District of Manipur state, Northeast India. Phytopharmacology 2:285–311Google Scholar
  23. Raghow R (1994) The role of extracellular matrix in postinflammatory wound healing and fibrosis. Fed Am Soc Exp Biol J 8:823–831Google Scholar
  24. Siddiqui S, Siddiqui BS, Adil Q, Begum S (1990) Constituents of Mirabilis jalapa. Fitoterapia 61:471Google Scholar
  25. Simpson T, Orgazaly B (1986) Useful medicinal plant, 1st edn. ELBS pp 555Google Scholar
  26. Singer AJ, Clark RAF (1999) Cutaneous wound healing. N Engl J Med 341:738–746PubMedCrossRefGoogle Scholar
  27. Stadelman WK, Digents AG, Tobin GR (1998) Physiology and healing dynamics of chronic cutaneous wounds. Am J Surg 176:39–47CrossRefGoogle Scholar
  28. Willey JM, Sherwood L, Woolverton CJ, Prescott LM, Harley JP, Klein DA (2002) Laboratory excerises in microbiology, 5th edn. The McGraw Hill CompanyGoogle Scholar
  29. Yang SW (2000) Three new phenolic compounds from a manipulated plant cell culture of Mirabilis jalapa. J Nat Prod 64:313–317CrossRefGoogle Scholar
  30. Yoganarasimhan SN (2000) Medicinal plants of India. Cyber Media, Tamil Nadu, p 357Google Scholar

Copyright information

© Institute of Korean Medicine, Kyung Hee University 2013

Authors and Affiliations

  • Jyotchna Gogoi
    • 1
  • Khonamai Sewa Nakhuru
    • 1
  • Pronobesh Chattophadhayay
    • 1
  • Ashok Kumar Rai
    • 2
  • Hemanta Kumar Gogoi
    • 1
  • Vijay Veer
    • 1
  1. 1.Defence Research LaboratoryTezpurIndia
  2. 2.Department of Life SciencesDibrugarh UniversityDibrugarhIndia

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