Determination of role of GABA and nitric oxide in anticonvulsant activity of Fragaria vesca L. ethanolic extract in chemically induced epilepsy in laboratory animals
- 376 Downloads
The aim of the present study was to investigate anticonvulsant effect of Fragaria vesca L. ethanolic etract in chemically induced epilepsy.Fragaria vesca L. ethanolic extract (FVEE) was prepared by extracting the fruit with 95 % ethanol. Seizures were induced by intraperitoneal injection of pentylenetetrazole, strychnine, isoniazid and subcutaneous injection of picrotoxin in male swiss albino mice. The in-vitro parameters were also determined including brain gamma amino butyric acid, nitric oxide.Single dose of PTZ, PTX, STR and INH resulted in resulted in hind-limb, tonic-clonic convulsion along with mortality in mice. Treatment with FVEE (250 and 500 mg/kg, p.o.) significantly and dose dependently (P < 0.01 and P < 0.001 respectively) attenuated onset of convulsion and duration of tonic convulsion induced by PTZ, PTX and INH. It also significantly inhibited the incidence of convulsion and mortality in the mice. Mice treated with FVEE (250 and 500 mg/kg, p.o.) showed significant elevation in brain GABA level and lowering in NO level as compared to vehicle treated mice. Mice treated with FVEE showed significant reduction in the locomotor activity. Treatment with FVEE failed to produce any significant change in STR induced convulsion.The findings of present investigation suggest that FVEE bears a profile similar to agent elevating the inhibitory neurotransmitter in brain and restoring the excitatory neurochemistry behind epileptic seizures. Further investigations are being carried out to characterize the biochemical nature to understand the active principles along with structure activity relationship.
KeywordsBrain GABA Epilepsy Fragaria vesca L. Isoniazid Nitric oxide Pentylenetetrazole Picrotoxin
The authors would like to acknowledge Prof. R. D. Patankar, Principal, Abhinav College of Pharmacy and Dr. S. L. Bodhankar, Head and Professor, Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Pune, India for their constant support and valuable suggestions for the research work. We are also thankful to Dr. Vasant Narke, Director, TOXINDIA, Pune, India for providing necessary facilities to carry out the study.
Disclosure of interest
There is no conflict of interest between any of the authors.
- Agrawal SS, Paridhavi M (2007) Essentials of crude drugs. In: Herbal drug technology. 1st ed. Hyderabad, India, Universities Press, pp 583–87.Google Scholar
- Extracta liquid-liquid extracts (1950) In: Pharmaceutical formulos. 1st ed. London, England, The Chemist and Druggist, p 183Google Scholar
- Farnsworth NR (1988) Screening plants for new medicines. In: Wilson EO (ed) Biodiversity, Chapter 9 Part II. National Academy Press, Washington DC, pp 83–97Google Scholar
- Khandelwal KR (2001) Preliminary phytochemicals screening, In: Practical pharmacognosy Techniques and Experiments. 8th edn. Nirali Publication, Pune, pp 149–156Google Scholar
- Khanna N, Bhalla S, Verma V, Sharma KK (2000) Modulatory effects of nifedipine and nimodipine in experimental convulsions. Indian J Pharmacol 32(6):347–352Google Scholar
- Kulkarni SK (1999) Hand book of experimental pharmacology. Vallabh Prakashan, New Delhi, pp 117–119Google Scholar
- Librowski T, Czarnecki R, Mendyk A, Jastrzebska M (2000) Influence of new monoterpenes homologues of GABA on the central nervous system activity in mice. Polish J Pharmacol 52:317–321Google Scholar
- Parmar NS, Shiv Prakash (2006) Screening methods in Pharmacology. Narosa Publishing House, New Delhi, pp 90–91Google Scholar
- Phillips R, Foy N (1990) London: Herbs Pan Books Ltd., Plants for a future: edible, medicinal and useful plants for a healthier world. Accessed on 25th May, 2008, at www.pfaf.org/index.htm
- Rang HP, Dale MM, Ritter JM, Moore PK (2005) Pharmacology, 5th edn. Churchill Livingstone, India, pp 456–473Google Scholar
- Rohkamm R (2004) Epilepsy: seizures and types. In: Colour Atlas of Neurology, Malestrom. Thieme Flexi book, 2nd edn. Georg Thieme Verlag, Stuttgart, pp 192–196Google Scholar
- Samini M, Shafaroodi H, Sehat VS (2005) Comparison of the effects of lindane and permethrin on pentylenetetrazole-induced clonic seizure in mice. Online J Vet Res 9(1):37–47Google Scholar
- Samren EB, Van Duijn CM, Koch S, Hiilesmaa VK, Klepel H, Bardy AH et al (1997) Maternal use of antiepileptic drugs and the risk of major congenital malfor malformations: a joint European prospective study of human teratogenesis associated with maternal epilepsy. Epilepsia 38:981–990PubMedCrossRefGoogle Scholar
- Vogel HG, Vogel WH (2002). In: Psychotropic and neurotropic activity. Drug discovery and evaluation: pharmacological assays, Editors: Vogel WH, Schlkens BA, Sandow J, et al., 2nd edn. Springer, Berlin, Germany, pp 422–424Google Scholar
- Zandieh A, Maleki F, Hajimirzabeigi A, Zandieh B, Khalilzadeh O et al (2010) Anticonvulsant effect of celecoxib on pentylenetetrazole-induced convulsion: Modulation by NO pathway. Acta Neurobiol Exp 70:390–397Google Scholar