Oriental Pharmacy and Experimental Medicine

, Volume 12, Issue 4, pp 255–264 | Cite as

Determination of role of GABA and nitric oxide in anticonvulsant activity of Fragaria vesca L. ethanolic extract in chemically induced epilepsy in laboratory animals

  • Mithun Vishwanath K. Patil
  • Amit D. Kandhare
  • Pinaki Ghosh
  • Sucheta D. BhiseEmail author
Research Article


The aim of the present study was to investigate anticonvulsant effect of Fragaria vesca L. ethanolic etract in chemically induced epilepsy.Fragaria vesca L. ethanolic extract (FVEE) was prepared by extracting the fruit with 95 % ethanol. Seizures were induced by intraperitoneal injection of pentylenetetrazole, strychnine, isoniazid and subcutaneous injection of picrotoxin in male swiss albino mice. The in-vitro parameters were also determined including brain gamma amino butyric acid, nitric oxide.Single dose of PTZ, PTX, STR and INH resulted in resulted in hind-limb, tonic-clonic convulsion along with mortality in mice. Treatment with FVEE (250 and 500 mg/kg, p.o.) significantly and dose dependently (P < 0.01 and P < 0.001 respectively) attenuated onset of convulsion and duration of tonic convulsion induced by PTZ, PTX and INH. It also significantly inhibited the incidence of convulsion and mortality in the mice. Mice treated with FVEE (250 and 500 mg/kg, p.o.) showed significant elevation in brain GABA level and lowering in NO level as compared to vehicle treated mice. Mice treated with FVEE showed significant reduction in the locomotor activity. Treatment with FVEE failed to produce any significant change in STR induced convulsion.The findings of present investigation suggest that FVEE bears a profile similar to agent elevating the inhibitory neurotransmitter in brain and restoring the excitatory neurochemistry behind epileptic seizures. Further investigations are being carried out to characterize the biochemical nature to understand the active principles along with structure activity relationship.


Anticonvulsant activity of Fragaria vesca L. ethanolic extract: Role of GABA and NO.


Brain GABA Epilepsy Fragaria vesca L. Isoniazid Nitric oxide Pentylenetetrazole Picrotoxin 



The authors would like to acknowledge Prof. R. D. Patankar, Principal, Abhinav College of Pharmacy and Dr. S. L. Bodhankar, Head and Professor, Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Pune, India for their constant support and valuable suggestions for the research work. We are also thankful to Dr. Vasant Narke, Director, TOXINDIA, Pune, India for providing necessary facilities to carry out the study.

Disclosure of interest

There is no conflict of interest between any of the authors.


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Copyright information

© Institute of Oriental Medicine, Kyung Hee University 2012

Authors and Affiliations

  • Mithun Vishwanath K. Patil
    • 1
  • Amit D. Kandhare
    • 2
  • Pinaki Ghosh
    • 2
  • Sucheta D. Bhise
    • 1
    Email author
  1. 1.Department of PharmaceuticsAbhinav College of PharmacyPuneIndia
  2. 2.Centre for Advanced Research in Pharmaceutical Sciences (CARPS), Department of Pharmacology, Poona College of PharmacyBharati Vidyapeeth Deemed UniversityPuneIndia

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