Formulation, standardization and pharmacological evaluation of a poly herbal traditional remedy -Ashwagandharishtam

  • Arun Rasheed
  • C. Roja
  • G. Avinash Kumar Reddy
  • B. Sravya Reddy
Research Article

Abstract

Ayurvedic formulations were considered the best and safer alternatives for synthetic drugs. But the demand to such products is diminishing due to lack of standardization. Ashwagandharishtam, a fermented kind of ayurvedic formulation, is used to treat epilepsy, nervous disorders, skin diseases, immune disorders and rheumatism. In the present study, the standardization of Ashwagandharishtam using sophisticated techniques were attempted for assessing the quality and safety of herbal products involved in it. It was formulated according to a working formula and its physico-chemical, pharmacological and biological parameters were determined. The results of physicochemical studies showed that the values were under acceptable limits as per the Ayurvedic Pharmacopoeial specifications. FTIR studies of formulation revealed the presence of functional groups similar to Ashwagandha powder. The heavy metal content in the formulation is determined using Atomic Absorption Spectroscopy. HPTLC reports proved the presence of biomarker, withanolide-A in ashwagandharishtam which helped in its chemical standardization. The anti-epileptic activity of ashwagandharishtam was evaluated by Maximal electro shock (MES) method in rats. It exhibited a dose dependent significant (P < 0.001) reduction in various phases of epileptic seizures when compared with that of standard phenytoin. It was also assessed for its anti inflammatory activity in carrageen induced paw edema method which showed a good activity comparable to standard thus making it biologically standardized.

Keywords

Standardization Ashwagandharishtam Withanolides Antiepileptic activity Anti-inflammatory activity 

References

  1. Arun Rasheed, Satyanarayana KV, Swetha GP, Sindhura M, Neelufarshama S, Avinashkumar G (2011) Standardization: accessing key for herbal drug safety assurance. J Pharm Res 4(5):1322–1325Google Scholar
  2. Chattopadhyay RN, Chattopadhyay R, Roy S, Moitra SK (1986) A simple method for plethysmometric measurement of paw volume of small laboratory animals in evaluation of anti inflammatory effects. Bull Calcutta School Trop Med 34:5–8Google Scholar
  3. Ghosh MN (2005) Fundamentals of experimental pharmacology. Hilton and company, KolkataGoogle Scholar
  4. Harborne JB (1998) Phytochemical methods. Chapman and Hall, LondonGoogle Scholar
  5. Indian pharmacopoeia, 1996, Vol-11 appendix 8.3, A87, Govt of India, NewdelhiGoogle Scholar
  6. Kalaiselvan V, Ankur KS, Falgun BP, Chainesh NS, Kalaivani M, Rajasekharan A (2010) Quality Assessment of different marketed brandsof Dasamoolaristam, an Ayurvedic formulation. Int J Ayur Res 1(1):10–13CrossRefGoogle Scholar
  7. Khalsa KP (2007) The practitioner’s perspective: introduction to Ayurvedic herbalism. J Altern Complement Med 13(10):1135–1150CrossRefGoogle Scholar
  8. Mohammad HM, Elisabeth M, Mercedes B, Rosa MC, Javier P (2009) Steroidal Lactones from Withania somnifera, an ancient plant for novel medicine. Molecules 14:2373–2393CrossRefGoogle Scholar
  9. Nadakarni, Indian Materia Medica, 1993, pp 1292Google Scholar
  10. Srivastava P, Tiwari N, Yadav AK, Kumar V, Shanker K, Verma RK, Gupta MM, Gupta AK, Khanuja SP (2008) Simultaneous quantification of withanolides in Withania somnifera by a validated high-performance thin-layer chromatographic method. JAOAC Int 91(5):1154–1161Google Scholar
  11. Wagner H, Bladt S (1996) Plant drug analysis: a thin layer chromatography atlas. Springer, Berlin HeidelbergGoogle Scholar
  12. WHO Guidelines: quality control methods for medicinal plant materials. Geneva. 3-70 (2004)Google Scholar

Copyright information

© Institute of Oriental Medicine, Kyung Hee University 2012

Authors and Affiliations

  • Arun Rasheed
    • 1
  • C. Roja
    • 2
  • G. Avinash Kumar Reddy
    • 2
  • B. Sravya Reddy
    • 2
  1. 1.Department of Pharmaceutical ChemistrySree Vidyanikethan College of PharmacyTirupatiIndia
  2. 2.Department of Pharmacognosy and PhytochemistrySree Vidyanikethan College of PharmacyTirupatiIndia

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