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Oriental Pharmacy and Experimental Medicine

, Volume 11, Issue 4, pp 271–277 | Cite as

Anti-inflammatory activity and antioxidant role of Zanthoxylum nitidum bark

  • Sanjib BhattacharyaEmail author
  • Pallab K. Haldar
  • Md. K. Zaman
Research Article

Abstract

In the present study, the methanol extract of stem bark of Zanthoxylum nitidum (ZNME) was evaluated for its anti-inflammatory activity in acute (carrageenan, histamine and serotonin induced rat paw oedema) and chronic model (cotton pellet induced granuloma) along with reference drug indomethacin (10 mg/kg body wt. p.o.). The ZNME (75 and 150 mg/kg body wt. p.o.) exhibited significant activity (p < 0.001) against all phlogistic agents employed in a dose dependent manner. In chronic model also the ZNME caused a significant (p < 0.001) and dose dependent inhibition of granuloma formation. The ZNME was evaluated for its antioxidant properties by 1,1- diphenyl-2-picryl hydrazyl (DPPH) radical scavenging assay and in vitro lipid peroxidation induced by the Fe2+-ascorbate system in rat liver homogenate. In DPPH radical scavenging assay, the ZNME demonstrated marked and dose dependent free radical scavenging effect and the mean inhibitory concentration (IC50) of the ZNME was found to be 77.8 μg/ml, while the ascorbic acid (reference) exhibited 43.7 μg/ml. The ZNME effectively inhibited the lipid peroxidation in a dose related manner showing the IC50 value of 297.9 μg/ml, whereas the catechin (reference) showed 46.64 μg/ml. These findings reveal that the ZNME possessed promising acute and chronic anti-inflammatory and in vitro antioxidant actions in the tested models.

Keywords

Zanthoxylum nitidum Anti-inflammatory Radical scavenging Lipid peroxidation 

Notes

Acknowledgement

The authors are thankful to the authority of Dibrugarh University, Dibrugarh, Assam 786004, India, for providing necessary facilities the present work.

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Copyright information

© Institute of Oriental Medicine, Kyung Hee University 2011

Authors and Affiliations

  • Sanjib Bhattacharya
    • 1
    Email author
  • Pallab K. Haldar
    • 2
  • Md. K. Zaman
    • 3
  1. 1.Pharmacognosy DivisionBengal School of Technology (A College of Pharmacy)HooghlyIndia
  2. 2.Department of Pharmaceutical TechnologyJadavpur UniversityKolkataIndia
  3. 3.Department of Pharmaceutical SciencesDibrugarh UniversityDibrugarhIndia

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