24-Epibrassinolide mitigates nickel toxicity in young Eucalyptus urophylla S.T. Blake plants: nutritional, physiological, biochemical, anatomical and morphological responses

  • Alaene Teixeira Ribeiro
  • Victor Pereira de Oliveira
  • Udson de Oliveira Barros Junior
  • Breno Ricardo Serrão da Silva
  • Bruno Lemos Batista
  • Allan Klynger da Silva LobatoEmail author
Research Paper


Key message

Our research revealed that 24-epibrassinolide alleviated nickel toxicity in young Eucalyptus urophylla plants, inducing benefits on nutritional, physiological, biochemical, anatomical and morphological responses.


Soil contamination by heavy metals may limit the Eucalyptus production. Disturbances caused by nickel (Ni) toxicity interfere with the absorption of other essential nutrients. 24-Epibrassinolide (EBR) is one form of brassinosteroid (BR) that provides benefits for plant metabolism under Ni toxicity.


The aim of this study was to determine whether exogenous EBR can improve ionic homeostasis by evaluating nutrient concentrations, anatomical characteristics and chlorophyll fluorescence in young Eucalyptus urophylla plants subjected to Ni toxicity.


The experiment was randomized into four treatments, including two Ni concentrations (0 and 600 μM Ni) and two 24-epibrassinolide concentrations (0 and 100 nM EBR).


EBR significantly reduced Ni contents. Plants exposed to Ni2+ and sprayed with steroid had increases in the Ca2+/Ni2+ and Mn2+/Ni2+ ratios in the leaves of 38% and 15%, respectively, compared with the same treatment without EBR. The treatment of Ni2+ toxicity + EBR presented an increase of 42% in effective quantum yield of PSII photochemistry, when compared with plants exposed to Ni without EBR. Ni toxicity induced negative effects on stomatal functionality, but EBR application mitigated these effects.


Benefits on effective quantum yield of PSII photochemistry after EBR spray can be related to increases in manganese contents. EBR reduced oxidative stress, alleviating the deleterious effects induced by Ni toxicity and inducing positive repercussions on antioxidant enzymes, photosynthetic pigments and biomass.


Eucalyptus urophylla Light capture Metal contamination Nutritional balance 24-epibrassinolide 





Intercellular CO2 concentration


Transpiration rate




Equatorial diameter of the stomata


Electrolyte leakage


Epidermis thickness from abaxial leaf side


Epidermis thickness from adaxial leaf side


Electron transport rate


Ratio between the apparent electron transport rate and net photosynthetic rate


Relative energy excess at the PSII level


Minimal fluorescence yield of the dark-adapted state




Maximal fluorescence yield of the dark-adapted state


Variable fluorescence


Maximal quantum yield of PSII photochemistry


Nonphotochemical quenching


Natural resistance-associated macrophage protein


Polar diameter of the stomata


Net photosynthetic rate


Instantaneous carboxylation efficiency


Palisade parenchyma thickness


Photochemical quenching


Root cortex diameter


Root dry matter


Root metaxylem diameter


Root endodermis thickness


Root epidermis thickness


Stomatal density


Stem dry matter


Stomatal functionality


Stomatal index


Spongy parenchyma thickness


Total dry matter

Total Chl

Total chlorophyll


Vascular cylinder diameter


Water-use efficiency


ZRT/IRT-like protein


Effective quantum yield of PSII photochemistry


Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.


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Copyright information

© INRA and Springer-Verlag France SAS, part of Springer Nature 2020

Authors and Affiliations

  • Alaene Teixeira Ribeiro
    • 1
  • Victor Pereira de Oliveira
    • 1
  • Udson de Oliveira Barros Junior
    • 1
  • Breno Ricardo Serrão da Silva
    • 1
  • Bruno Lemos Batista
    • 2
  • Allan Klynger da Silva Lobato
    • 1
    Email author
  1. 1.Núcleo de Pesquisa Vegetal Básica e AplicadaUniversidade Federal Rural da AmazôniaParagominasBrazil
  2. 2.Centro de Ciências Naturais e HumanasUniversidade Federal do ABCSão PauloBrazil

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