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Annals of Forest Science

, 75:74 | Cite as

Low within-population genetic diversity and high genetic differentiation among populations of the endangered plant Tetracentron sinense Oliver revealed by inter-simple sequence repeat analysis

  • Shan Li
  • Xiaohong Gan
  • Hongyan Han
  • Xuemei Zhang
  • Zhongqiong Tian
Research Paper

Abstract

Key message

Tetracentron sinense Oliver, an endangered species from China, displays a low within-population genetic diversity and high genetic differentiation among populations, and the existing populations could be divided into three conservation and management units.

Context

The endangered tree Tetracentron sinense Oliver has great value; however, little is known regarding the within-population genetic diversity and differentiation among T. sinense populations.

Aims

We examined the genetic diversity and differentiation of T. sinense wild populations, and we tested the effect of small-size population on the level of genetic diversity within these populations.

Methods

Using inter-simple sequence repeat (ISSR), we assessed the genetic variation and structure among 174 individuals from 26 natural populations of T. sinense sampled across its distribution range in China.

Results

The ISSR primers yielded 180 amplified loci (123 were polymorphic). At the species level, the percentage of polymorphic loci (PPL), Nei’s gene diversity (H), and Shannon’s information index (I) were 68.3%, 0.196 and 0.300, respectively. The average population level PPL was 20.0%, and the Na, Ne, H, and I were 1.20, 1.13, 0.076, and 0.112, respectively. AMOVA revealed high genetic differentiation among populations (52.0% of total variance, P = 0.001), consistent with the gene differentiation coefficient (Gst = 0.607) and gene flow (Nm = 0.326). The 174 individuals of the 26 T. sinense populations clustered into three groups, and T. sinense geographic and genetic distance were significantly correlated.

Conclusions

T. sinense exhibited intermediate within-species genetic diversity, indicating preserved evolutionary potential. The low within-population genetic diversity and high genetic differentiation among T. sinense populations may be one of important factors causing endangerment. Three conservation units were determined based on genetic difference and structure. Inter-population introduction of individuals within units via appropriate propagation and seedling management might be an effective strategy for increasing T. sinense within-population genetic diversity and population size.

Keywords

Tetracentron sinense Oliver Genetic variation Genetic structure Molecular marker ISSR Conservation strategy, China 

Abbreviations

AFLP

amplified fragment length polymorphisms

cpDNA

chroloplast DNA

Gst

coefficient of genetic differentiation

H

Nei’s gene diversity

Hs

gene diversity within populations

Ht

total gene diversity

I

Shannon’s information index

ISSR

inter-simple sequence repeat

Na

observed number of alleles

Ne

effective number of alleles

Nm

gene flow among populations

PPL

the percentage of polymorphic loci

RAPD

random amplified polymorphic DNA

SGS

spatial genetic structure

SSR

simple sequence repeat

Notes

Acknowledgements

We thank the following peoples in each Nature Reserve Authority (NRA) for sample collecting: Zhirong Gu and Guorong Wei in Badagongshan NRA and Longping Tang in Sunhuangshan NRA of Hunan province, Shuanzhu Dong in Taibaishan NRA of Shanxi province, Liming Chen in Tangjiahe NRA and Dahai Zhu in Longxi-Hongkou NRA of Sichuan province, Aicai Nie in Wufeng Houhe NRA of Hubei province, and Ma in Baishuijiang NRA of Gansu province.

Funding

This work was supported by National Natural Science Foundation of China (NO.31370367), the Applied Basic Research Project of Sichuan Province, China (No.2017JY0164) and the Meritocracy Research Funds of China West Normal University (No. 17YC325).

Compliance with ethical standards

The State Forestry Administration of the People’s Republic of China granted permissions to Professor Xiaohong Gan for using the endangered species of plant (Tetracentron sinense).

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© INRA and Springer-Verlag France SAS, part of Springer Nature 2018

Authors and Affiliations

  • Shan Li
    • 1
    • 2
  • Xiaohong Gan
    • 1
    • 2
  • Hongyan Han
    • 1
    • 2
  • Xuemei Zhang
    • 1
    • 2
  • Zhongqiong Tian
    • 1
    • 2
  1. 1.Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life ScienceChina West Normal UniversityNanchongChina
  2. 2.Institute of Plant Adaptation and Utilization in Southwest MountainChina West Normal UniversityNanchongChina

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