Annals of Forest Science

, 75:72 | Cite as

Selection of arbuscular mycorrhizal fungal strains to improve Casuarina equisetifolia L. and Casuarina glauca Sieb. tolerance to salinity

  • Pape Ibrahima DjighalyEmail author
  • Nathalie Diagne
  • Mariama Ngom
  • Daouda Ngom
  • Valérie Hocher
  • Dioumacor Fall
  • Diégane Diouf
  • Laurent Laplaze
  • Sergio Svistoonoff
  • Antony Champion
Research Paper


Key message

Selection of the best salt-tolerant combination of Casuarina sp. and arbuscular mycorrhizal fungi (AMF) is one of the key criteria for successful setup of saline land rehabilitation program.


Land salinization is a serious problem worldwide that mainly leads to soil degradation and reduces crop productivity. These degraded areas could be rehabilitated by planting salt-tolerant species like Casuarina glauca Sieb. and Casuarina equisetifolia L. These are pioneer plants, able to form symbiotic associations with arbuscular mycorrhizal fungi (AMF), ectomycorrhizal fungi (EMF), and nitrogen-fixing bacteria.


The aim of this study was to select the highest salt-tolerant combination of Casuarina/AMF that can be used for the rehabilitation of lands degraded by salinity.


C. equisetifolia and C. glauca were grown in sandy sterile soil in the greenhouse and inoculated separately with Rhizophagus fasciculatus (Thaxt.) C. Walker & A. Schüßler, Rhizophagus aggregatus (N.C. Schenck & G.S. Sm.) C. Walker, and Rhizophagus intraradices (N.C. Schenck & G.S. Sm.) C. Walker & A. Schüßler. After confirming the establishment of a symbiosis, the plants were watered with gradually increasing concentrations of saline solution. After harvest, size and biomass of the seedlings, root colonization by AMF, and AMF metabolic activities were evaluated.


A larger growth was obtained in the two species when the individuals were inoculated with R. fasciculatus. Root colonization rates did not differ among fungal species, but fungal metabolic activities were higher in mycorrhizal roots of C. glauca plants inoculated with R. fasciculatus.


Among the three mycorrhizal fungi, R. fasciculatus was more efficient in association with Casuarinaceae species under salt stress. Our results suggest that selection of appropriate fungal strains is crucial to improve plant performance in saline soils.


Salinity Arbuscular mycorrhizal fungi C. glauca C. equisetifolia Rehabilitation Salt-affected land 



This work was supported by the International Foundation for Sciences (IFS, no. AD/22680), the Academy of Sciences for the Developing World (TWAS, no. 11-214 RG/BIO/AF/AC I), The “Fonds d’Impulsion de la Recherche Scientifique et Technique” (FIRST) of the Ministry of Higher Education and Research of Senegal, and the “Laboratoire Mixte International Adaptation des Plantes et microorganismes associés aux Stress Environnementaux” (LAPSE). P. I. Djighaly was granted by “Institut de Recherche pour le Développement” (IRD) through its ARTS PhD program.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13595_2018_747_Fig5_ESM.png (1.3 mb)
Supplemental fig. 1

Effect of NaCl on root colonization of Casuarina a. Staining of Uvitex 2B in Casuarina roots not exposed to NaCl (arrow indicates arbuscular mycorrhizae); b. Casuarina roots subjected to 150 mM NaCl; c. Plant subjected to 300 mM NaCl, cw: cell wall, h: intracellular hyphae, Bars=100μM. (PNG 1367 kb)

13595_2018_747_MOESM1_ESM.eps (462 kb)
High resolution image (EPS 462 kb)


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Copyright information

© INRA and Springer-Verlag France SAS, part of Springer Nature 2018

Authors and Affiliations

  • Pape Ibrahima Djighaly
    • 1
    • 2
    • 3
    • 4
    Email author return OK on get
  • Nathalie Diagne
    • 2
    • 4
  • Mariama Ngom
    • 1
    • 2
    • 5
  • Daouda Ngom
    • 3
  • Valérie Hocher
    • 6
  • Dioumacor Fall
    • 1
    • 2
    • 7
  • Diégane Diouf
    • 1
    • 2
    • 5
  • Laurent Laplaze
    • 2
    • 8
  • Sergio Svistoonoff
    • 1
    • 2
    • 6
  • Antony Champion
    • 1
    • 2
    • 8
  1. 1.Laboratoire Commun de Microbiologie (LCM) Institut de Recherche pour le Développement/Institut Sénégalais de Recherches Agricoles/Université Cheikh Anta Diop, (IRD/ISRA/UCAD), Centre de Recherche de Bel AirDakarSenegal
  2. 2.Laboratoire Mixte International Adaptation des Plantes et Microorganismes Associés aux Stress Environnementaux (LAPSE), Centre de Recherche de Bel AirDakarSenegal
  3. 3.Département d’AgroforesterieUniversité Assane Seck de ZiguinchorZiguinchorSenegal
  4. 4.Centre National de Recherches Agronomiques (ISRA/CNRA)BambeySenegal
  5. 5.Département de Biologie VégétaleUniversité Cheikh Anta Diop de DakarDakarSenegal
  6. 6.Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM), (IRD/INRA/CIRAD/Université de Montpellier/Supagro), IRD TA A-82/JCampus International de BaillarguetMontpellier CEDEX 5France
  7. 7.Centre National de Recherches Forestières (ISRA/CNRF)DakarSenegal
  8. 8.Institut de Recherche pour le Développement (IRD), Unité Mixte de Recherche DIADE (Diversité Adaptation et Développement des plantes)Montpellier CEDEX 5France

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